- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Liu, C.
- Articles by Lustig, A. J.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Liu, C.
- Articles by Lustig, A. J.
Genetics, Vol 143, 81-93, Copyright © 1996
INVESTIGATIONS |
Genetic Analysis of Rap1p/Sir3p Interactions in Telomeric and HML Silencing in Saccharomyces cerevisiae
C. Liu and A. J. Lustig
Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021
We have identified three SIR3 suppressors of the telomeric silencing defects conferred by missense mutations within the Rap1p C-terminal tail domain (aa 800-827). Each SIR3 suppressor was also capable of suppressing a rap1 allele (rap1-21), which deletes the 28 aa C-terminal tail domain, but none of the suppressors restored telomeric silencing to a 165 amino acid truncation allele. These data suggest a Rap1p site for Sir3p association between the two truncation points (aa 664-799). In SIR3 suppressor strains lacking the Rap1p C-terminal tail domain, the presence of a second intragenic mutation within the rap1s domain (aa 727-747), enhanced silencing 30-300-fold. These data suggest a competition between Sir3p and factors that interfere with silencing for association in the rap1(s) domain. rap1-21 strains containing both wild-type Sir3p and either of the Sir3 suppressor proteins displayed a 400-4000-fold increase in telomeric silencing over rap1-21 strains carrying either Sir3p suppressor in the absence of wild-type Sir3p. We propose that this telomere-specific synergism is mediated in part through stabilization of Rap1p/Sir3p telomeric complexes by Sir3p-Sir3p interactions.
This article has been cited by other articles:
 |
|
 |
|
  H. Liaw and A. J. Lustig Sir3 C-Terminal Domain Involvement in the Initiation and Spreading of Heterochromatin Mol. Cell. Biol., October 15, 2006; 26(20): 7616 - 7631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Hou, J. R. Danzer, C. A. Fox, and J. L. Keck Structure of the Sir3 protein bromo adjacent homology (BAH) domain from S. cerevisiae at 1.95 A resolution. Protein Sci., May 1, 2006; 15(5): 1182 - 1186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Connelly, P. Yuan, H.-C. Hsu, Z. Li, R.-M. Xu, and R. Sternglanz Structure and Function of the Saccharomyces cerevisiae Sir3 BAH Domain Mol. Cell. Biol., April 15, 2006; 26(8): 3256 - 3265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. De Las Penas, S.-J. Pan, I. Castano, J. Alder, R. Cregg, and B. P. Cormack Virulence-related surface glycoproteins in the yeast pathogen Candida glabrata are encoded in subtelomeric clusters and subject to RAP1- and SIR-dependent transcriptional silencing Genes & Dev., September 15, 2003; 17(18): 2245 - 2258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Luo, M. A. Vega-Palas, and M. Grunstein Rap1-Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast Genes & Dev., June 15, 2002; 16(12): 1528 - 1539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Carmen, L. Milne, and M. Grunstein Acetylation of the Yeast Histone H4 N Terminus Regulates Its Binding to Heterochromatin Protein SIR3 J. Biol. Chem., February 8, 2002; 277(7): 4778 - 4781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Moretti and D. Shore Multiple Interactions in Sir Protein Recruitment by Rap1p at Silencers and Telomeres in Yeast Mol. Cell. Biol., December 1, 2001; 21(23): 8082 - 8094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Stone, C. Reifsnyder, M. McVey, B. Gazo, and L. Pillus Two Classes of sir3 Mutants Enhance the sir1 Mutant Mating Defect and Abolish Telomeric Silencing in Saccharomyces cerevisiae Genetics, June 1, 2000; 155(2): 509 - 522. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Park and A. J. Lustig Telomere Structure Regulates the Heritability of Repressed Subtelomeric Chromatin in Saccharomyces cerevisiae Genetics, February 1, 2000; 154(2): 587 - 598. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Park, J. Hanish, and A. J. Lustig Sir3p Domains Involved in the Initiation of Telomeric Silencing in Saccharomyces cerevisiae Genetics, November 1, 1998; 150(3): 977 - 986. [Abstract] [Full Text]
|
|
|
|
|
|
M. Gotta, F. Palladino, and S. M. Gasser Functional Characterization of the N Terminus of Sir3p Mol. Cell. Biol., October 1, 1998; 18(10): 6110 - 6120. [Abstract] [Full Text]
|
|
|
|
|
|
B. D. Bourns, M. K. Alexander, A. M. Smith, and V. A. Zakian Sir Proteins, Rif Proteins, and Cdc13p Bind Saccharomyces Telomeres In Vivo Mol. Cell. Biol., September 1, 1998; 18(9): 5600 - 5608. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Marcand, E. Gilson, and D. Shore A Protein-Counting Mechanism for Telomere Length Regulation in Yeast Science, February 14, 1997; 275(5302): 986 - 990. [Abstract] [Full Text]
|
|
|
|
|
|
S Strahl-Bolsinger, A Hecht, K Luo, and M Grunstein SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast. Genes & Dev., January 1, 1997; 11(1): 83 - 93. [Abstract] [PDF]
|
|