- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Lohe, A. R.
- Articles by Hartl, D. L.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Lohe, A. R.
- Articles by Hartl, D. L.
Genetics, Vol 143, 365-374, Copyright © 1996
INVESTIGATIONS |
Germline Transformation of Drosophila virilis With the Transposable Element mariner
A. R. Lohe and D. L. Hartl
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge Massachusetts 02138
An important goal in molecular genetics has been to identify a transposable element that might serve as an efficient transformation vector in diverse species of insects. The transposable element mariner occurs naturally in a wide variety of insects. Although virtually all mariner elements are nonfunctional, the Mos1 element isolated from Drosophila mauritiana is functional. Mos1 was injected into the pole-cell region of embryos of D. virilis, which last shared a common ancestor with D. mauritiana 40 million years ago. Mos1 PCR fragments were detected in several pools of DNA from progeny of injected animals, and backcross lines were established. Because G(0) lines were pooled, possibly only one transformation event was actually obtained, yielding a minimum frequency of 4%. Mos1 segregated in a Mendelian fashion, demonstrating chromosomal integration. The copy number increased by spontaneous mobilization. In situ hybridization confirmed multiple polymorphic locations of Mos1. Integration results in a characteristic 2-bp TA duplication. One Mos1 element integrated into a tandem array of 370-bp repeats. Some copies may have integrated into heterochromatin, as evidenced by their ability to support PCR amplification despite absence of a signal in Southern and in situ hybridizations.
This article has been cited by other articles:
 |
|
 |
|
  H. D. Tinsley Profile of Daniel L. Hartl PNAS, May 29, 2007; 104(22): 9111 - 9113. [Full Text] [PDF]
|
|
|
|
 |
|
 E. G. Barry, D. J. Witherspoon, and D. J. Lampe A Bacterial Genetic Screen Identifies Functional Coding Sequences of the Insect mariner Transposable Element Famar1 Amplified From the Genome of the Earwig, Forficula auricularia Genetics, February 1, 2004; 166(2): 823 - 833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Lampe, D. J. Witherspoon, F. N. Soto-Adames, and H. M. Robertson Recent Horizontal Transfer of Mellifera Subfamily Mariner Transposons into Insect Lineages Representing Four Different Orders Shows that Selection Acts Only During Horizontal Transfer Mol. Biol. Evol., April 1, 2003; 20(4): 554 - 562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Kapetanaki, T. G. Loukeris, I. Livadaras, and C. Savakis High frequencies of Minos transposon mobilization are obtained in insects by using in vitro synthesized mRNA as a source of transposase Nucleic Acids Res., August 1, 2002; 30(15): 3333 - 3340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. KAMINSKI, M. R. HUBER, J. B. SUMMERS, and M. B. WARD Design of a nonviral vector for site-selective, efficient integration into the human genome FASEB J, August 1, 2002; 16(10): 1242 - 1247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Lozovsky, D. Nurminsky, E. A. Wimmer, and D. L. Hartl Unexpected Stability of mariner Transgenes in Drosophila Genetics, February 1, 2002; 160(2): 527 - 535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Hartl Discovery of the Transposable Element Mariner Genetics, February 1, 2001; 157(2): 471 - 476. [Full Text]
|
|
|
|
|
|
L. R. O. Tosi and S. M. Beverley cis and trans factors affecting Mos1 mariner evolution and transposition in vitro, and its potential for functional genomics Nucleic Acids Res., February 1, 2000; 28(3): 784 - 790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Lampe, B. J. Akerley, E. J. Rubin, J. J. Mekalanos, and H. M. Robertson Hyperactive transposase mutants of the Himar1 mariner transposon PNAS, September 28, 1999; 96(20): 11428 - 11433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Handler, S. D. McCombs, M. J. Fraser, and S. H. Saul The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly PNAS, June 23, 1998; 95(13): 7520 - 7525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Lampe, T. E. Grant, and H. M. Robertson Factors Affecting Transposition of the Himar1 mariner Transposon in Vitro Genetics, May 1, 1998; 149(1): 179 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Erickson and T. Cline Key aspects of the primary sex determination mechanism are conserved across the genus Drosophila Development, January 8, 1998; 125(16): 3259 - 3268. [Abstract] [PDF]
|
|
|
|
|
|
F. Catteruccia, T. Nolan, C. Blass, H.-M. Muller, A. Crisanti, F. C. Kafatos, and T. G. Loukeris Toward Anopheles transformation: Minos element activity in anopheline cells and embryos PNAS, February 29, 2000; 97(5): 2157 - 2162. [Abstract] [Full Text] [PDF]
|
|