- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Ferea, T. L.
- Articles by Bowman, B. J.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Ferea, T. L.
- Articles by Bowman, B. J.
Genetics, Vol 143, 147-154, Copyright © 1996
INVESTIGATIONS |
The Vacuolar ATPase of Neurospora crassa Is Indispensable: Inactivation of the vma-1 Gene by Repeat-Induced Point Mutation
T. L. Ferea and B. J. Bowman
Department of Biology, Sinsheimer Laboratories, University of California, Santa Cruz, California 95064
To analyze the phenotype of cells lacking the vacuolar ATPase, we inactivated the vma-1 gene, which encodes the catalytic subunit of the enzyme. Because preliminary experiments suggested the vma-1 gene was essential, we developed a method of simultaneously inactivating the gene and complementing it with a functional copy. We call this method repeat-induced point mutation (RIP) & Rescue. Two strains, both of which contained an extra copy of the vma-1 gene, were mated. Progeny that had inherited a functional copy of the gene at an ectopic site in the genome were selected. In some of these progeny the endogenous vma-1 gene had been altered by the RIP process. Sequencing showed the endogenous vma-1 gene had been inactivated by multiple point mutations. Progeny from strains with an inactive endogenous vma-1 gene were inviable unless a functional copy of the gene cosegregated, indicating that the vacuolar ATPase is essential in Neurospora crassa.
This article has been cited by other articles:
 |
|
 |
|
  F. Quattrocchio, W. Verweij, A. Kroon, C. Spelt, J. Mol, and R. Koes PH4 of Petunia Is an R2R3 MYB Protein That Activates Vacuolar Acidification through Interactions with Basic-Helix-Loop-Helix Transcription Factors of the Anthocyanin Pathway PLANT CELL, May 1, 2006; 18(5): 1274 - 1291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. W. Beyenbach and H. Wieczorek The V-type H+ ATPase: molecular structure and function, physiological roles and regulation J. Exp. Biol., February 15, 2006; 209(4): 577 - 589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Hilson, J. Allemeersch, T. Altmann, S. Aubourg, A. Avon, J. Beynon, R. P. Bhalerao, F. Bitton, M. Caboche, B. Cannoot, et al. Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications Genome Res., October 1, 2004; 14(10b): 2176 - 2189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Bowman, R. Kendle, and B. J. Bowman Disruption of vma-1, the Gene Encoding the Catalytic Subunit of the Vacuolar H+-ATPase, Causes Severe Morphological Changes in Neurospora crassa J. Biol. Chem., January 7, 2000; 275(1): 167 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N Nelson, N Perzov, A Cohen, K Hagai, V Padler, and H Nelson The cellular biology of proton-motive force generation by V-ATPases J. Exp. Biol., January 1, 2000; 203(1): 89 - 95. [Abstract]
|
|
|
|
|
|
E. Bowman and B. Bowman Cellular role of the V-ATPase in Neurospora crassa: analysis of mutants resistant to concanamycin or lacking the catalytic subunit A J. Exp. Biol., January 1, 2000; 203(1): 97 - 106. [Abstract]
|
|
|
|
|
|
P. J. Plant, M. F. Manolson, S. Grinstein, and N. Demaurex Alternative Mechanisms of Vacuolar Acidification in H+-ATPase-deficient Yeast J. Biol. Chem., December 24, 1999; 274(52): 37270 - 37279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Schumacher, D. Vafeados, M. McCarthy, H. Sze, T. Wilkins, and J. Chory The Arabidopsis det3 mutant reveals a central role for the vacuolar H+-ATPase in plant growth and development Genes & Dev., December 15, 1999; 13(24): 3259 - 3270. [Abstract] [Full Text]
|
|
|
|
|
|
C. Forster, M. A. Santos, S. Ruffert, R. Kramer, and J. L. Revuelta Physiological Consequence of Disruption of the VMA1 Gene in the Riboflavin Overproducer Ashbya gossypii J. Biol. Chem., April 2, 1999; 274(14): 9442 - 9448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Zhang, K. J. Parra, J. Liu, and P. M. Kane Characterization of a Temperature-sensitive Yeast Vacuolar ATPase Mutant with Defects in Actin Distribution and Bud Morphology J. Biol. Chem., July 17, 1998; 273(29): 18470 - 18480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Bowman, F. J. O'Neill, and B. J. Bowman Mutations of pma-1, the Gene Encoding the Plasma Membrane H+-ATPase of Neurospora crassa, Suppress Inhibition of Growth by Concanamycin A, a Specific Inhibitor of Vacuolar ATPases J. Biol. Chem., June 6, 1997; 272(23): 14776 - 14786. [Abstract] [Full Text] [PDF]
|
|