- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Therond, P.
- Articles by Busson, D.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Therond, P.
- Articles by Busson, D.
Genetics, Vol 142, 1181-1198, Copyright © 1996
INVESTIGATIONS |
Functional Domains of Fused, a Serine-Threonine Kinase Required for Signaling in Drosophila
P. Therond, G. Alves, B. Limbourg-Bouchon, H. Tricoire, E. Guillemet, J. Brissard-Zahraoui, C. Lamour-Isnard and D. Busson
Present address: G. W. Hooper Foundation, HSW 1542, University of California, San Francisco, CA 94143.
fused (fu) is a segment-polarity gene encoding a putative serine-threonine kinase. In a wild-type context, all fu mutations display the same set of phenotypes. Nevertheless, mutations of the Suppressor of fused [Su(fu)] gene define three classes of alleles (fu0, fuI, fuII). Here, we report the molecular analysis of known fu mutations and the generation of new alleles by in vitro mutagenesis. We show that the Fused (Fu) protein functions in vivo as a kinase. The N-terminal kinase and the extreme C-terminal domains are necessary for Fu(+) activity while a central region appears to be dispensable. We observe a striking correlation between the molecular lesions of fu mutations and the phenotype displayed in their interaction with Su(fu). Indeed, fuI alleles which are suppressed by Su(fu) mutations are defined by inframe alterations of the N-terminal catalytic domain whereas the C-terminal domain is missing or altered in all fuII alleles. An unregulated FuII protein, which can be limited to the 80 N-terminal amino acids of the kinase domain, would be responsible for the neomorphic costal-2 phenotype displayed by the fuII-Su(fu) interaction. We propose that the Fu C-terminal domain can differentially regulate the Fu catalytic domain according to cell position in the parasegment.
This article has been cited by other articles:
 |
|
 |
|
  Y. Liu, X. Cao, J. Jiang, and J. Jia Fused Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation Genes & Dev., August 1, 2007; 21(15): 1949 - 1963. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-H. Chen, N. Gao, T. Kawakami, and P.-T. Chuang Mice Deficient in the Fused Homolog Do Not Exhibit Phenotypes Indicative of Perturbed Hedgehog Signaling during Embryonic Development Mol. Cell. Biol., August 15, 2005; 25(16): 7042 - 7053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Merchant, M. Evangelista, S.-M. Luoh, G. D. Frantz, S. Chalasani, R. A. D. Carano, M. van Hoy, J. Ramirez, A. K. Ogasawara, L. M. McFarland, et al. Loss of the Serine/Threonine Kinase Fused Results in Postnatal Growth Defects and Lethality Due to Progressive Hydrocephalus Mol. Cell. Biol., August 15, 2005; 25(16): 7054 - 7068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ascano Jr. and D. J. Robbins An Intramolecular Association between Two Domains of the Protein Kinase Fused Is Necessary for Hedgehog Signaling Mol. Cell. Biol., December 1, 2004; 24(23): 10397 - 10405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Besse and A.-M. Pret Apoptosis-mediated cell death within the ovarian polar cell lineage of Drosophila melanogaster Development, March 1, 2003; 130(5): 1017 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Besse, D. Busson, and A.-M. Pret Fused-dependent Hedgehog signal transduction is required for somatic cell differentiation during Drosophila egg chamber formation Development, September 1, 2002; 129(17): 4111 - 4124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Guichard, S. Srinivasan, G. Zimm, and E. Bier A screen for dominant mutations applied to components in the Drosophila EGF-R pathway PNAS, March 19, 2002; 99(6): 3752 - 3757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ascano Jr., K. E. Nybakken, J. Sosinski, M. A. Stegman, and D. J. Robbins The Carboxyl-Terminal Domain of the Protein Kinase Fused Can Function as a Dominant Inhibitor of Hedgehog Signaling Mol. Cell. Biol., March 1, 2002; 22(5): 1555 - 1566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Fukumoto, R. Watanabe-Fukunaga, K. Fujisawa, S. Nagata, and R. Fukunaga The Fused Protein Kinase Regulates Hedgehog-stimulated Transcriptional Activation in Drosophila Schneider 2 Cells J. Biol. Chem., October 12, 2001; 276(42): 38441 - 38448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Vieira and B. Charlesworth Evidence for Selection at the fused Locus of Drosophila virilis Genetics, August 1, 2000; 155(4): 1701 - 1709. [Abstract] [Full Text]
|
|
|
|
|
|
N Methot and K Basler Suppressor of fused opposes hedgehog signal transduction by impeding nuclear accumulation of the activator form of Cubitus interruptus Development, January 9, 2000; 127(18): 4001 - 4010. [Abstract] [PDF]
|
|
|
|
|
|
P. Therond, B Limbourg Bouchon, A Gallet, F Dussilol, T Pietri, M van den Heuvel, and H Tricoire Differential requirements of the fused kinase for hedgehog signalling in the Drosophila embryo Development, January 9, 1999; 126(18): 4039 - 4051. [Abstract] [PDF]
|
|
|
|
|
|
J. T. Ohlmeyer and D. Kalderon Dual pathways for induction of wingless expression by protein kinase A and Hedgehog in Drosophila embryos Genes & Dev., September 1, 1997; 11(17): 2250 - 2258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.L. Johnson and M.P. Scott Control of Cell Growth and Fate by patched Genes Cold Spring Harb Symp Quant Biol, January 1, 1997; 62(0): 205 - 215. [Abstract] [PDF]
|
|