- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Rinckel, L. A.
- Articles by Garfinkel, D. J.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Rinckel, L. A.
- Articles by Garfinkel, D. J.
Genetics, Vol 142, 761-776, Copyright © 1996
INVESTIGATIONS |
Influences of Histone Stoichiometry on the Target Site Preference of Retrotransposons Ty1 and Ty2 in Saccharomyces cerevisiae
L. A. Rinckel and D. J. Garfinkel
Gene Regulation and Chromosome Biology Laboratory, NCI-Frederick Cancer Research and Development Center, ABL-Basic Research Program, Frederick, Maryland 21702-1201
In Saccharomyces cerevisiae, the target site specificity of the retrotransposon Ty1 appears to involve the Ty integration complex recognizing chromatin structures. To determine whether changes in chromatin structure affect Ty1 and Ty2 target site preference, we analyzed Ty transposition at the CAN1 locus in mutants containing altered levels of histone proteins. A {Delta}hta1-htb1 mutant with decreased levels of H2A and H2B histone proteins showed a pattern of Ty1 and Ty2 insertions at CAN1 that was significantly different from that of both the wild-type and a {Delta}hta2-htb2 mutant, which does not have altered histone protein levels. Altered levels of H2A and H2B proteins disrupted a dramatic orientation bias in the CAN1 promoter region. In the wild-type strains, few Ty1 and Ty2 insertions in the promoter region were oriented opposite to the direction of CAN1 transcription. In the {Delta}hta1-htb1 background, however, numerous Ty1 and Ty2 insertions were in the opposite orientation clustered within the TATA region. This altered insertion pattern does not appear to be due to a bias caused by selecting canavanine resistant isolates in the different HTA1-HTB1 backgrounds. Our results suggest that reduced levels of histone proteins alter Ty target site preference and disrupt an asymmetric Ty insertion pattern.
This article has been cited by other articles:
 |
|
 |
|
  K. M. Nyswaner, M. A. Checkley, M. Yi, R. M. Stephens, and D. J. Garfinkel Chromatin-Associated Genes Protect the Yeast Genome From Ty1 Insertional Mutagenesis Genetics, January 1, 2008; 178(1): 197 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Garfinkel, K. M. Stefanisko, K. M. Nyswaner, S. P. Moore, J. Oh, and S. H. Hughes Retrotransposon Suicide: Formation of Ty1 Circles and Autointegration via a Central DNA Flap J. Virol., December 15, 2006; 80(24): 11920 - 11934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Mou, A. E. Kenny, and M. J. Curcio Hos2 and Set3 Promote Integration of Ty1 Retrotransposons at tRNA Genes in Saccharomyces cerevisiae Genetics, April 1, 2006; 172(4): 2157 - 2167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Gelbart, N. Bachman, J. Delrow, J. D. Boeke, and T. Tsukiyama Genome-wide identification of Isw2 chromatin-remodeling targets by localization of a catalytically inactive mutant Genes & Dev., April 15, 2005; 19(8): 942 - 954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Griffith, L. E. Coleman, A. S. Raymond, S. G. Goodson, W. S. Pittard, C. Tsui, and S. E. Devine Functional Genomics Reveals Relationships Between the Retrovirus-Like Ty1 Element and Its Host Saccharomyces cerevisiae Genetics, July 1, 2003; 164(3): 867 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Behrens, J. Hayles, and P. Nurse Fission yeast retrotransposon Tf1 integration is targeted to 5' ends of open reading frames Nucleic Acids Res., December 1, 2000; 28(23): 4709 - 4716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-S. Lee, L. Bi, D. J. Garfinkel, and A. M. Bailis Nucleotide Excision Repair/TFIIH Helicases Rad3 and Ssl2 Inhibit Short-Sequence Recombination and Ty1 Retrotransposition by Similar Mechanisms Mol. Cell. Biol., April 1, 2000; 20(7): 2436 - 2445. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. S. Mysore, J. Nam, and S. B. Gelvin An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration PNAS, January 18, 2000; 97(2): 948 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Huang, J. Y. Hong, C. L. Burck, and S. W. Liebman Host Genes That Affect the Target-Site Distribution of the Yeast Retrotransposon Ty1 Genetics, April 1, 1999; 151(4): 1393 - 1407. [Abstract] [Full Text]
|
|
|
|
|
|
Z. Qian, H. Huang, J. Y. Hong, C. L. Burck, S. D. Johnston, J. Berman, A. Carol, and S. W. Liebman Yeast Ty1 Retrotransposition Is Stimulated by a Synergistic Interaction between Mutations in Chromatin Assembly Factor I and Histone Regulatory Proteins Mol. Cell. Biol., August 1, 1998; 18(8): 4783 - 4792. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. M. Kim, S. Vanguri, J. D. Boeke, A. Gabriel, and D. F. Voytas Transposable Elements and Genome Organization: A Comprehensive Survey of Retrotransposons Revealed by the Complete Saccharomyces cerevisiae Genome Sequence Genome Res., May 1, 1998; 8(5): 464 - 478. [Abstract] [Full Text]
|
|
|
|
|
|
B.-S. Lee, C. P. Lichtenstein, B. Faiola, L. A. Rinckel, W. Wysock, M. J. Curcio, and D. J. Garfinkel Posttranslational Inhibition of Ty1 Retrotransposition by Nucleotide Excision Repair/Transcription Factor TFIIH Subunits Ssl2p and Rad3p Genetics, April 1, 1998; 148(4): 1743 - 1761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zou and D. F. Voytas Silent chromatin determines target preference of the Saccharomyces retrotransposon Ty5 PNAS, July 8, 1997; 94(14): 7412 - 7416. [Abstract] [Full Text] [PDF]
|
|