Genetic Requirements for the Single-Strand Annealing Pathway of Double-Strand Break Repair in Saccharomyces cerevisiae

INVESTIGATIONS

Genetic Requirements for the Single-Strand Annealing Pathway of Double-Strand Break Repair in Saccharomyces cerevisiae

E. L. Ivanov, N. Sugawara, J. Fishman-Lobell and J. E. Haber
Present address: Transkaryotic Therapies, Inc., Cambridge, MA 02139.

HO endonuclease-induced double-strand breaks (DSBs) within a direct duplication of Escherichia coli lacZ genes are repaired either by gene conversion or by single-strand annealing (SSA), with >80% being SSA. Previously it was demonstrated that the RAD52 gene is required for DSB-induced SSA. In the present study, the effects of other genes belonging to the RAD52 epistasis group were analyzed. We show that RAD51, RAD54, RAD55, and RAD57 genes are not required for SSA irrespective of whether recombination occurred in plasmid or chromosomal DNA. In both plasmid and chromosomal constructs with homologous sequences in direct orientation, the proportion of SSA events over gene conversion was significantly elevated in the mutant strains. However, gene conversion was not affected when the two lacZ sequences were in inverted orientation. These results suggest that there is a competition between SSA and gene conversion processes that favors SSA in the absence of RAD51, RAD54, RAD55 and RAD57. Mutations in RAD50 and XRS2 genes do not prevent the completion, but markedly retard the kinetics, of DSB repair by both mechanisms in the lacZ direct repeat plasmid, a result resembling the effects of these genes during mating-type (MAT) switching.

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				Y. Wu, J. S. Siino, T. Sugiyama, and S. C. Kowalczykowski The DNA Binding Preference of RAD52 and RAD59 Proteins: IMPLICATIONS FOR RAD52 AND RAD59 PROTEIN FUNCTION IN HOMOLOGOUS RECOMBINATION J. Biol. Chem., December 29, 2006; 281(52): 40001 - 40009. [Abstract] [Full Text] [PDF]

				J. Lopes, C. Ribeyre, and A. Nicolas Complex Minisatellite Rearrangements Generated in the Total or Partial Absence of Rad27/hFEN1 Activity Occur in a Single Generation and Are Rad51 and Rad52 Dependent. Mol. Cell. Biol., September 1, 2006; 26(17): 6675 - 6689. [Abstract] [Full Text] [PDF]

				M. Papamichos-Chronakis, J. E. Krebs, and C. L. Peterson Interplay between Ino80 and Swr1 chromatin remodeling enzymes regulates cell cycle checkpoint adaptationin response to DNA damage Genes & Dev., September 1, 2006; 20(17): 2437 - 2449. [Abstract] [Full Text] [PDF]

				H. Tsubouchi and G. S. Roeder Budding yeast Hed1 down-regulates the mitotic recombination machinery when meiotic recombination is impaired. Genes & Dev., July 1, 2006; 20(13): 1766 - 1775. [Abstract] [Full Text] [PDF]

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				M. Clerici, D. Mantiero, G. Lucchini, and M. P. Longhese The Saccharomyces cerevisiae Sae2 Protein Promotes Resection and Bridging of Double Strand Break Ends J. Biol. Chem., November 18, 2005; 280(46): 38631 - 38638. [Abstract] [Full Text] [PDF]

				H. Yan, J. McCane, T. Toczylowski, and C. Chen Analysis of the Xenopus Werner syndrome protein in DNA double-strand break repair J. Cell Biol., October 24, 2005; 171(2): 217 - 227. [Abstract] [Full Text] [PDF]

				S. Houghtaling, A. Newell, Y. Akkari, T. Taniguchi, S. Olson, and M. Grompe Fancd2 functions in a double strand break repair pathway that is distinct from non-homologous end joining Hum. Mol. Genet., October 15, 2005; 14(20): 3027 - 3033. [Abstract] [Full Text] [PDF]

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				E. Schildkraut, C. A. Miller, and J. A. Nickoloff Gene conversion and deletion frequencies during double-strand break repair in human cells are controlled by the distance between direct repeats Nucleic Acids Res., March 14, 2005; 33(5): 1574 - 1580. [Abstract] [Full Text] [PDF]

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				K. Nakanishi, Y.-G. Yang, A. J. Pierce, T. Taniguchi, M. Digweed, A. D. D'Andrea, Z.-Q. Wang, and M. Jasin Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair PNAS, January 25, 2005; 102(4): 1110 - 1115. [Abstract] [Full Text] [PDF]

				A.N.J. TUTT, C.J. LORD, N. MCCABE, H. FARMER, N. TURNER, N.M. MARTIN, S.P. JACKSON, G.C.M. SMITH, and A. ASHWORTH Exploiting the DNA Repair Defect in BRCA Mutant Cells in the Design of New Therapeutic Strategies for Cancer Cold Spring Harb Symp Quant Biol, January 1, 2005; 70(0): 139 - 148. [Abstract] [PDF]

				J. M. Stark, A. J. Pierce, J. Oh, A. Pastink, and M. Jasin Genetic Steps of Mammalian Homologous Repair with Distinct Mutagenic Consequences Mol. Cell. Biol., November 1, 2004; 24(21): 9305 - 9316. [Abstract] [Full Text] [PDF]

				L. D. Langston and L. S. Symington Gene targeting in yeast is initiated by two independent strand invasions PNAS, October 26, 2004; 101(43): 15392 - 15397. [Abstract] [Full Text] [PDF]