- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by McNeil, J. B.
- Articles by Pearlman, R. E.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by McNeil, J. B.
- Articles by Pearlman, R. E.
Genetics, Vol 142, 371-381, Copyright © 1996
INVESTIGATIONS |
In Vivo Analysis of Folate Coenzymes and Their Compartmentation in Saccharomyces cerevisiae
J. B. McNeil, A. L. Bognar and R. E. Pearlman
Department of Biology, York University, Toronto, Ontario M3J 1P3, Canada
In eukaryotes, enzymes responsible for the interconversion of one-carbon units exist in parallel in both mitochondria and the cytoplasm. Strains of Saccharomyces cerevisiae were constructed that possess combinations of gene disruptions at the SHM1 [mitochondrial serine hydroxymethyltransferase (SHMTm)], SHM2 [cytoplasmic SHMT (SHMTc)], MIS1 [mitochondrial C(1)-tetrahydrofolate synthase (C(1)-THFSm)], ADE3 [cytoplasmic C(1)-THF synthase (C(1)-THFSc)], GCV1 [glycine cleavage system (GCV) protein T], and the GLY1 (involved in glycine synthesis) loci. Analysis of the in vivo growth characteristics and phenotypes was used to determine the contribution to cytoplasmic nucleic acid and amino acid anabolism by the mitochondrial enzymes involved in the interconversion of folate coenzymes. The data indicate that mitochondria transport formate to the cytoplasmic compartment and mitochondrial synthesis of formate appears to rely primarily on SHMTm rather than the glycine cleavage system. The glycine cleavage system and SHMTm cooperate to specifically synthesize serine. With the inactivation of SHM1, however, the glycine cleavage system can make an observable contribution to the level of mitochondrial formate. Inactivation of SHM1, SHM2 and ADE3 is required to render yeast auxotrophic for TMP and methionine, suggesting that TMP synthesized in mitochondria may be available to the cytoplasmic compartment.
This article has been cited by other articles:
 |
|
 |
|
  D. A. Scott, S. M. Hickerson, T. J. Vickers, and S. M. Beverley The Role of the Mitochondrial Glycine Cleavage Complex in the Metabolism and Virulence of the Protozoan Parasite Leishmania major J. Biol. Chem., January 4, 2008; 283(1): 155 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Rebora, B. Laloo, and B. Daignan-Fornier Revisiting Purine-Histidine Cross-Pathway Regulation in Saccharomyces cerevisiae: A Central Role for a Small Molecule Genetics, May 1, 2005; 170(1): 61 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Bhatt, M. Y. Khan, and V. Bhakuni The C-terminal domain of dimeric serine hydroxymethyltransferase plays a key role in stabilization of the quaternary structure and cooperative unfolding of protein: Domain swapping studies with enzymes having high sequence identity Protein Sci., August 1, 2004; 13(8): 2184 - 2195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Guldener, G. J. Koehler, C. Haussmann, A. Bacher, J. Kricke, D. Becher, and J. H. Hegemann Characterization of the Saccharomyces cerevisiae Fol1 Protein: Starvation for C1 Carrier Induces Pseudohyphal Growth Mol. Biol. Cell, August 1, 2004; 15(8): 3811 - 3828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Gelling, M. D. W. Piper, S.-P. Hong, G. D. Kornfeld, and I. W. Dawes Identification of a Novel One-carbon Metabolism Regulon in Saccharomyces cerevisiae J. Biol. Chem., February 20, 2004; 279(8): 7072 - 7081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chaturvedi and V. Bhakuni Unusual Structural, Functional, and Stability Properties of Serine Hydroxymethyltransferase from Mycobacterium tuberculosis J. Biol. Chem., October 17, 2003; 278(42): 40793 - 40805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yang and U. T. Meier Genetic Interaction between a Chaperone of Small Nucleolar Ribonucleoprotein Particles and Cytosolic Serine Hydroxymethyltransferase J. Biol. Chem., June 20, 2003; 278(26): 23553 - 23560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Albers, V. Laize, A. Blomberg, S. Hohmann, and L. Gustafsson Ser3p (Yer081wp) and Ser33p (Yil074cp) Are Phosphoglycerate Dehydrogenases in Saccharomyces cerevisiae J. Biol. Chem., March 14, 2003; 278(12): 10264 - 10272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Palkova, F. Devaux, M. Ricicova, L. Minarikova, S. Le Crom, and C. Jacq Ammonia Pulses and Metabolic Oscillations Guide Yeast Colony Development Mol. Biol. Cell, November 1, 2002; 13(11): 3901 - 3914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Gombert, M. Moreira dos Santos, B. Christensen, and J. Nielsen Network Identification and Flux Quantification in the Central Metabolism of Saccharomyces cerevisiae under Different Conditions of Glucose Repression J. Bacteriol., February 15, 2001; 183(4): 1441 - 1451. [Abstract] [Full Text]
|
|
|
|
|
|
H. Cherest, D. Thomas, and Y. Surdin-Kerjan Polyglutamylation of Folate Coenzymes Is Necessary for Methionine Biosynthesis and Maintenance of Intact Mitochondrial Genome in Saccharomyces cerevisiae J. Biol. Chem., May 5, 2000; 275(19): 14056 - 14063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Nagarajan and R. K. Storms Molecular Characterization of GCV3, the Saccharomyces cerevisiae Gene Coding for the Glycine Cleavage System Hydrogen Carrier Protein J. Biol. Chem., February 14, 1997; 272(7): 4444 - 4450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Piper, S.-P. Hong, G. E. Ball, and I. W. Dawes Regulation of the Balance of One-carbon Metabolism in Saccharomyces cerevisiae J. Biol. Chem., September 29, 2000; 275(40): 30987 - 30995. [Abstract] [Full Text] [PDF]
|
|