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Genetics, Vol 142, 173-177, Copyright © 1996
INVESTIGATIONS |
Germline Transformation of Drosophila virilis Mediated by the Transposable Element hobo
E. R. Lozovskaya, D. I. Nurminsky, D. L. Hartl and D. T. Sullivan
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138
A laboratory strain of Drosophila virilis was genetically transformed with a hobo vector carrying the miniwhite cassette using a helper plasmid with an hsp70-driven hobo transposase-coding sequence. The rate of transformation was 0.5% per fertile G0 animal. Three transgenic insertions were cloned and characterized and found to be authentic hobo insertions. These results, together with the known wide-spread distribution of hobo in diverse insect species, suggest that hobo and related transposable elements may be of considerable utility in the germline transformation of insects other than D. melanogaster.
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