Mutations Modulating Raf Signaling in Drosophila Eye Development

INVESTIGATIONS

Mutations Modulating Raf Signaling in Drosophila Eye Development

B. J. Dickson, A. van-der-Straten, M. Dominguez and E. Hafen
Present address: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720.

The R7 fate is specified during Drosophila eye development by an inductive signal transduced intracellularly via the Raf kinase. We have performed a genetic screen for dominant mutations that alter the efficiency with which cells respond to a constitutively activated Raf kinase. Such mutations may affect genes involved in signal transduction downstream of Raf. We have isolated 44 mutations that define eight genes. One of these encodes a mitogen-activated protein kinase homologue; another is a putative target gene of this signaling pathway. We present the results of this screen in detail, as well as a preliminary genetic analysis of the six loci still to be characterized molecularly.

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				S. Szuplewski, B. Kottler, and R. Terracol The Drosophila bZIP transcription factor Vrille is involved in hair and cell growth Development, August 15, 2003; 130(16): 3651 - 3662. [Abstract] [Full Text] [PDF]

				J. Li and W. X. Li Drosophila Gain-of-Function Mutant RTK Torso Triggers Ectopic Dpp and STAT Signaling Genetics, May 1, 2003; 164(1): 247 - 258. [Abstract] [Full Text] [PDF]

				T. L. Tootle, P. S. Lee, and I. Rebay CRM1-mediated nuclear export and regulated activity of the Receptor Tyrosine Kinase antagonist YAN require specific interactions with MAE Development, March 1, 2003; 130(5): 845 - 857. [Abstract] [Full Text] [PDF]

				M. A. Su, R. G. Wisotzkey, and S. J. Newfeld A Screen for Modifiers of decapentaplegic Mutant Phenotypes Identifies lilliputian, the Only Member of the Fragile-X/Burkitt's Lymphoma Family of Transcription Factors in Drosophila melanogaster Genetics, February 1, 2001; 157(2): 717 - 725. [Abstract] [Full Text]

				F Wittwer, A van der Straten, K Keleman, B. Dickson, and E Hafen Lilliputian: an AF4/FMR2-related protein that controls cell identity and cell growth Development, January 3, 2001; 128(5): 791 - 800. [Abstract] [PDF]

				A. Tang, T. Neufeld, G. Rubin, and H. Muller Transcriptional regulation of cytoskeletal functions and segmentation by a novel maternal pair-rule gene, lilliputian Development, January 3, 2001; 128(5): 801 - 813. [Abstract] [PDF]

				A. M. Huang and G. M. Rubin A Misexpression Screen Identifies Genes That Can Modulate RAS1 Pathway Signaling in Drosophila melanogaster Genetics, November 1, 2000; 156(3): 1219 - 1230. [Abstract] [Full Text]

				M. Therrien, D. K. Morrison, A. M. Wong, and G. M. Rubin A Genetic Screen for Modifiers of a Kinase Suppressor of Ras-Dependent Rough Eye Phenotype in Drosophila Genetics, November 1, 2000; 156(3): 1231 - 1242. [Abstract] [Full Text]

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				B Kuang, S. Wu, Y Shin, L Luo, and P Kolodziej split ends encodes large nuclear proteins that regulate neuronal cell fate and axon extension in the Drosophila embryo Development, January 4, 2000; 127(7): 1517 - 1529. [Abstract] [PDF]

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				L. Yue, T. L. Karr, D. F. Nathan, H. Swift, S. Srinivasan, and S. Lindquist Genetic Analysis of Viable Hsp90 Alleles Reveals a Critical Role in Drosophila Spermatogenesis Genetics, March 1, 1999; 151(3): 1065 - 1079. [Abstract] [Full Text]

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				E. Wiellette, K. Harding, K. Mace, M. Ronshaugen, F. Wang, and W McGinnis spen encodes an RNP motif protein that interacts with Hox pathways to repress the development of head-like sclerites in the Drosophila trunk Development, January 12, 1999; 126(23): 5373 - 5385. [Abstract] [PDF]

				Y Kimura, S L Rutherford, Y Miyata, I Yahara, B C Freeman, L Yue, R I Morimoto, and S Lindquist Cdc37 is a molecular chaperone with specific functions in signal transduction. Genes & Dev., July 15, 1997; 11(14): 1775 - 1785. [Abstract] [PDF]

				H C Chang and G M Rubin 14-3-3 epsilon positively regulates Ras-mediated signaling in Drosophila. Genes & Dev., May 1, 1997; 11(9): 1132 - 1139. [Abstract] [PDF]

				X Dong, K H Zavitz, B J Thomas, M Lin, S Campbell, and S L Zipursky Control of G1 in the developing Drosophila eye: rca1 regulates Cyclin A. Genes & Dev., January 1, 1997; 11(1): 94 - 105. [Abstract] [PDF]