- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Colot, V.
- Articles by Rossignol, J. L.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Colot, V.
- Articles by Rossignol, J. L.
Genetics, Vol 141, 1299-1314, Copyright © 1995
INVESTIGATIONS |
Isolation of the Ascobolus immersus Spore Color Gene b2 and Study in Single Cells of Gene Silencing by Methylation Induced Premeiotically
V. Colot and J. L. Rossignol
Institut de Genetique et Microbiologie, Centre National de la Recherche Scientifique Unite de Recherche Associee 1354, Universite Paris-sud, F-91405 Orsay Cedex, France
The ascomycete Ascobolus immersus has been extensively used as a model system for the genetic study of meiotic recombination. More recently, an epigenetic process, known as methylation induced premeiotically (MIP), that acts on duplicated sequences has been discovered in A. immersus and has raised a new interest in this fungus. To try and extend these studies, we have now cloned the A. immersus spore color gene b2, a well characterized recombination hot-spot. Isolation of the whole gene was verified by physical mapping of four large b2 alterations, followed by transformation and mutant rescue of a null b2 allele. Transformation was also used to duplicate b2 and subject it to MIP. As a result, we were able for the first time to observe gene silencing as early as just after meiosis and in single cells. Furthermore, we have found evidence for a modulating effect of MIP on b2 expression, depending on the region of the gene that is duplicated and hence subjected to MIP.
This article has been cited by other articles:
 |
|
 |
|
  V. Colot, V. Haedens, and J.-L. Rossignol Extensive, Nonrandom Diversity of Excision Footprints Generated by Ds-Like Transposon Ascot-1 Suggests New Parallels with V(D)J Recombination Mol. Cell. Biol., July 1, 1998; 18(7): 4337 - 4346. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. A. Jeddeloh, J. Bender, and E. J. Richards The DNA methylation locus DDM1 is required for maintenance of gene silencing in Arabidopsis Genes & Dev., June 1, 1998; 12(11): 1714 - 1725. [Abstract] [Full Text]
|
|
|
|
|
|
L. Maloisel and J.-L. Rossignol Suppression of crossing-over by DNA methylation in Ascobolus Genes & Dev., May 1, 1998; 12(9): 1381 - 1389. [Abstract] [Full Text]
|
|