Genetics, Vol 141, 1275-1285, Copyright © 1995

INVESTIGATIONS

Suppressors of a Saccharomyces cerevisiae pkc1 Mutation Identify Alleles of the Phosphatase Gene PTC1 and of a Novel Gene Encoding a Putative Basic Leucine Zipper Protein

K. N. Huang and L. S. Symington
Institute of Cancer Research and Department of Microbiology, Columbia University College of Physicians nd Surgeons, New York, New York 10032

The PKC1 gene product, protein kinase C, regulates a mitogen-activated protein kinase (MAPK) cascade, which is implicated in cell wall metabolism. Previously, we identified the pkc1-4 allele in a screen for mutants with increased rates of recombination, indicating that PKC1 may also regulate DNA metabolism. The pkc1-4 allele also conferred a temperature-sensitive (ts) growth defect. Extragenic suppressors were isolated that suppress both the ts and hyperrecombination phenotypes conferred by the pkc1-4 mutation. Eight of these suppressors fell into two complementation groups, designated KCS1 and KCS2. KCS1 was cloned and found to encode a novel protein with homology to the basic leucine zipper family of transcription factors. KCS2 is allelic with PTC1, a previously identified type 2C serine/threonine protein phosphatase. Although mutation of either KCS1 or PTC1 causes little apparent phenotype, the kcs1{delta} ptc1{delta} double mutant fails to grow at 30{deg}. Furthermore, the ptc1 deletion mutation is synthetically lethal in combination with a mutation in MPK1, which encodes a MAPK homologue proposed to act in the PKC1 pathway. Because PTC1 was initially isolated as a component of the Hog1p MAPK pathway, it appears that these two MAPK cascades share a common regulatory feature.

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