Roles of ABF1, NPL3, and YCL54 in Silencing in Saccharomyces cerevisiae

INVESTIGATIONS

Roles of ABF1, NPL3, and YCL54 in Silencing in Saccharomyces cerevisiae

S. Loo, P. Laurenson, M. Foss, A. Dillin and J. Rine
Department of Molecular and Cell Biology, Division of Genetics, University of California, Berkeley, California 94720

A sensitized genetic screen was carried out to identify essential genes involved in silencing in Saccharomyces cerevisiae. This screen identified temperature-sensitive alleles of ORC2 and ORC5, as described elsewhere, and ABF1, NPL3, and YCL54, as described here. Alleles of ABF1 that caused silencing defects provided the genetic proof of Abf1p's role in silencing. The roles of Npl3p and Ycl54p are less clear. These proteins did not act exclusively through any one of the three protein binding sites of the HMR-E silencer. Unlike the orc2, orc5, and abf1 mutations that were isolated in the same (or a similar) screen for silencing mutants, neither temperature-sensitive mutation in NPL3 or YCL54 caused overt replication defects.

This article has been cited by other articles:

M. Vujcic, M. Shroff, and K. K. Singh
Genetic Determinants of Mitochondrial Response to Arsenic in Yeast Saccharomyces cerevisiae
Cancer Res., October 15, 2007; 67(20): 9740 - 9749.
[Abstract] [Full Text] [PDF]

T. Miyake, J. Reese, C. M. Loch, D. T. Auble, and R. Li
Genome-wide Analysis of ARS (Autonomously Replicating Sequence) Binding Factor 1 (Abf1p)-mediated Transcriptional Regulation in Saccharomyces cerevisiae
J. Biol. Chem., August 13, 2004; 279(33): 34865 - 34872.
[Abstract] [Full Text] [PDF]

M. Oki, L. Valenzuela, T. Chiba, T. Ito, and R. T. Kamakaka
Barrier Proteins Remodel and Modify Chromatin To Restrict Silenced Domains
Mol. Cell. Biol., March 1, 2004; 24(5): 1956 - 1967.
[Abstract] [Full Text] [PDF]

C. XU, P. A. HENRY, A. SETYA, and M. F. HENRY
In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p
RNA, June 1, 2003; 9(6): 746 - 759.
[Abstract] [Full Text] [PDF]

T. Miyake, C. M. Loch, and R. Li
Identification of a Multifunctional Domain in Autonomously Replicating Sequence-Binding Factor 1 Required for Transcriptional Activation, DNA Replication, and Gene Silencing
Mol. Cell. Biol., January 15, 2002; 22(2): 505 - 516.
[Abstract] [Full Text] [PDF]

S. U. Åström, A. Kegel, J. O. O. Sjöstrand, and J. Rine
Kluyveromyces lactis Sir2p Regulates Cation Sensitivity and Maintains a Specialized Chromatin Structure at the Cryptic {alpha}-Locus
Genetics, September 1, 2000; 156(1): 81 - 91.
[Abstract] [Full Text]

L. Pintard, D. Kressler, and B. Lapeyre
Spb1p Is a Yeast Nucleolar Protein Associated with Nop1p and Nop58p That Is Able To Bind S-Adenosyl-L-Methionine In Vitro
Mol. Cell. Biol., February 15, 2000; 20(4): 1370 - 1381.
[Abstract] [Full Text]

A. E. McBride, V. H. Weiss, H. K. Kim, J. M. Hogle, and P. A. Silver
Analysis of the Yeast Arginine Methyltransferase Hmt1p/Rmt1p and Its in Vivo Function. COFACTOR BINDING AND SUBSTRATE INTERACTIONS
J. Biol. Chem., February 4, 2000; 275(5): 3128 - 3136.
[Abstract] [Full Text] [PDF]

S. H. Reed, M. Akiyama, B. Stillman, and E. C. Friedberg
Yeast autonomously replicating sequence binding factor is involved in nucleotide excision repair
Genes & Dev., December 1, 1999; 13(23): 3052 - 3058.
[Abstract] [Full Text]

E. Y. Xu, S. Kim, K. Replogle, J. Rine, and D. H. Rivier
Identification of SAS4 and SAS5, Two Genes That Regulate Silencing in Saccharomyces cerevisiae
Genetics, September 1, 1999; 153(1): 13 - 23.
[Abstract] [Full Text]

S. L. Sanders, M. Gentzsch, W. Tanner, and I. Herskowitz
O-Glycosylation of Axl2/Bud10p by Pmt4p Is Required for Its Stability, Localization, and Function in Daughter Cells
J. Cell Biol., June 14, 1999; 145(6): 1177 - 1188.
[Abstract] [Full Text] [PDF]

D. H. Rivier, J. L. Ekena, and J. Rine
HMR-I Is an Origin of Replication and a Silencer in Saccharomyces cerevisiae
Genetics, February 1, 1999; 151(2): 521 - 529.
[Abstract] [Full Text]

C Englund, A. Uv, R Cantera, L. Mathies, M. Krasnow, and C Samakovlis
adrift, a novel bnl-induced Drosophila gene, required for tracheal pathfinding into the CNS
Development, January 4, 1999; 126(7): 1505 - 1514.
[Abstract] [PDF]

S. C. Schroeder and P. A. Weil
Genetic Tests of the Role of Abf1p in Driving Transcription of the Yeast TATA Box Bindng Protein-encoding Gene, SPT15
J. Biol. Chem., July 31, 1998; 273(31): 19884 - 19891.
[Abstract] [Full Text] [PDF]

R. T. Kamakaka and J. Rine
Sir- and Silencer-Independent Disruption of Silencing in Saccharomyces by Sas10p
Genetics, June 1, 1998; 149(2): 903 - 914.
[Abstract] [Full Text] [PDF]

C. Wu, K. Weiss, C. Yang, M. A. Harris, B.-K. Tye, C. S. Newlon, R. T. Simpson, and J. E. Haber
Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching
Genes & Dev., June 1, 1998; 12(11): 1726 - 1737.
[Abstract] [Full Text]

S. U. Astrom and J. Rine
Theme and Variation Among Silencing Proteins in Saccharomyces cerevisiae and Kluyveromyces lactis
Genetics, March 1, 1998; 148(3): 1021 - 1029.
[Abstract] [Full Text] [PDF]

S. Enomoto and J. Berman
Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci
Genes & Dev., January 15, 1998; 12(2): 219 - 232.
[Abstract] [Full Text]

C. W. Siebel and C. Guthrie
The essential yeast RNA binding protein Npl3p is methylated
PNAS, November 26, 1996; 93(24): 13641 - 13646.
[Abstract] [Full Text] [PDF]

S Marcand, S W Buck, P Moretti, E Gilson, and D Shore
Silencing of genes at nontelomeric sites in yeast is controlled by sequestration of silencing factors at telomeres by Rap 1 protein.
Genes & Dev., June 1, 1996; 10(11): 1297 - 1309.
[Abstract] [PDF]

M S Lee, M Henry, and P A Silver
A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export.
Genes & Dev., May 15, 1996; 10(10): 1233 - 1246.
[Abstract] [PDF]

S G Holmes and J R Broach
Silencers are required for inheritance of the repressed state in yeast.
Genes & Dev., April 15, 1996; 10(8): 1021 - 1032.
[Abstract] [PDF]

				T. Miyake, J. Reese, C. M. Loch, D. T. Auble, and R. Li Genome-wide Analysis of ARS (Autonomously Replicating Sequence) Binding Factor 1 (Abf1p)-mediated Transcriptional Regulation in Saccharomyces cerevisiae J. Biol. Chem., August 13, 2004; 279(33): 34865 - 34872. [Abstract] [Full Text] [PDF]

				M. Oki, L. Valenzuela, T. Chiba, T. Ito, and R. T. Kamakaka Barrier Proteins Remodel and Modify Chromatin To Restrict Silenced Domains Mol. Cell. Biol., March 1, 2004; 24(5): 1956 - 1967. [Abstract] [Full Text] [PDF]

				C. XU, P. A. HENRY, A. SETYA, and M. F. HENRY In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p RNA, June 1, 2003; 9(6): 746 - 759. [Abstract] [Full Text] [PDF]

				T. Miyake, C. M. Loch, and R. Li Identification of a Multifunctional Domain in Autonomously Replicating Sequence-Binding Factor 1 Required for Transcriptional Activation, DNA Replication, and Gene Silencing Mol. Cell. Biol., January 15, 2002; 22(2): 505 - 516. [Abstract] [Full Text] [PDF]

				S. U. Åström, A. Kegel, J. O. O. Sjöstrand, and J. Rine Kluyveromyces lactis Sir2p Regulates Cation Sensitivity and Maintains a Specialized Chromatin Structure at the Cryptic {alpha}-Locus Genetics, September 1, 2000; 156(1): 81 - 91. [Abstract] [Full Text]

				L. Pintard, D. Kressler, and B. Lapeyre Spb1p Is a Yeast Nucleolar Protein Associated with Nop1p and Nop58p That Is Able To Bind S-Adenosyl-L-Methionine In Vitro Mol. Cell. Biol., February 15, 2000; 20(4): 1370 - 1381. [Abstract] [Full Text]

				A. E. McBride, V. H. Weiss, H. K. Kim, J. M. Hogle, and P. A. Silver Analysis of the Yeast Arginine Methyltransferase Hmt1p/Rmt1p and Its in Vivo Function. COFACTOR BINDING AND SUBSTRATE INTERACTIONS J. Biol. Chem., February 4, 2000; 275(5): 3128 - 3136. [Abstract] [Full Text] [PDF]

				S. H. Reed, M. Akiyama, B. Stillman, and E. C. Friedberg Yeast autonomously replicating sequence binding factor is involved in nucleotide excision repair Genes & Dev., December 1, 1999; 13(23): 3052 - 3058. [Abstract] [Full Text]

				E. Y. Xu, S. Kim, K. Replogle, J. Rine, and D. H. Rivier Identification of SAS4 and SAS5, Two Genes That Regulate Silencing in Saccharomyces cerevisiae Genetics, September 1, 1999; 153(1): 13 - 23. [Abstract] [Full Text]

				S. L. Sanders, M. Gentzsch, W. Tanner, and I. Herskowitz O-Glycosylation of Axl2/Bud10p by Pmt4p Is Required for Its Stability, Localization, and Function in Daughter Cells J. Cell Biol., June 14, 1999; 145(6): 1177 - 1188. [Abstract] [Full Text] [PDF]

				D. H. Rivier, J. L. Ekena, and J. Rine HMR-I Is an Origin of Replication and a Silencer in Saccharomyces cerevisiae Genetics, February 1, 1999; 151(2): 521 - 529. [Abstract] [Full Text]

				C Englund, A. Uv, R Cantera, L. Mathies, M. Krasnow, and C Samakovlis adrift, a novel bnl-induced Drosophila gene, required for tracheal pathfinding into the CNS Development, January 4, 1999; 126(7): 1505 - 1514. [Abstract] [PDF]

				S. C. Schroeder and P. A. Weil Genetic Tests of the Role of Abf1p in Driving Transcription of the Yeast TATA Box Bindng Protein-encoding Gene, SPT15 J. Biol. Chem., July 31, 1998; 273(31): 19884 - 19891. [Abstract] [Full Text] [PDF]

				R. T. Kamakaka and J. Rine Sir- and Silencer-Independent Disruption of Silencing in Saccharomyces by Sas10p Genetics, June 1, 1998; 149(2): 903 - 914. [Abstract] [Full Text] [PDF]

				C. Wu, K. Weiss, C. Yang, M. A. Harris, B.-K. Tye, C. S. Newlon, R. T. Simpson, and J. E. Haber Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching Genes & Dev., June 1, 1998; 12(11): 1726 - 1737. [Abstract] [Full Text]

				S. U. Astrom and J. Rine Theme and Variation Among Silencing Proteins in Saccharomyces cerevisiae and Kluyveromyces lactis Genetics, March 1, 1998; 148(3): 1021 - 1029. [Abstract] [Full Text] [PDF]

				S. Enomoto and J. Berman Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci Genes & Dev., January 15, 1998; 12(2): 219 - 232. [Abstract] [Full Text]

				C. W. Siebel and C. Guthrie The essential yeast RNA binding protein Npl3p is methylated PNAS, November 26, 1996; 93(24): 13641 - 13646. [Abstract] [Full Text] [PDF]

				S Marcand, S W Buck, P Moretti, E Gilson, and D Shore Silencing of genes at nontelomeric sites in yeast is controlled by sequestration of silencing factors at telomeres by Rap 1 protein. Genes & Dev., June 1, 1996; 10(11): 1297 - 1309. [Abstract] [PDF]

				M S Lee, M Henry, and P A Silver A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export. Genes & Dev., May 15, 1996; 10(10): 1233 - 1246. [Abstract] [PDF]

				S G Holmes and J R Broach Silencers are required for inheritance of the repressed state in yeast. Genes & Dev., April 15, 1996; 10(8): 1021 - 1032. [Abstract] [PDF]