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Genetics, Vol 141, 805-812, Copyright © 1995
INVESTIGATIONS |
{chi} Recombination Activity in Phage {lambda} Decays as a Function of Genetic Distance
R. S. Myers, M. M. Stahl and F. W. Stahl
Present address: Department of Biochemistry, Faculty of Natural Sciences, University of the West Indies, St. Augustine, Trinidad.
In Escherichia coli, {chi} is a recombination hotspot that stimulates RecBCD-dependent exchange at and to one side of itself. {chi} activity is highest at {chi} and decreases with distance from {chi}. The decrease in {chi} activity may be a simple property of the physical distance over which {chi} can stimulate recombination. Alternatively, the decay in {chi} activity with distance may reflect the high likelihood that {chi}-stimulated recombination occurs in a single {chi}-proximal act, to the exclusion of additional {chi}-stimulated exchanges more distal to {chi}. To test the models, we determined if {chi} activity decreases as a function of physical distance (i.e., DNA base pairs) or genetic distance (homologous DNA base pairs). Our results indicate that {chi} activity decays as a function of genetic distance. In addition, we found that the sbcB gene product (exonuclease I, a 3' -> 5' ssDNA exonuclease) modulates the distance over which {chi} can act. In contrast, the recJ gene product (a 5' -> 3' ssDNA exonuclease) does not alter the decay of {chi} activity.
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