Spatial and Temporal Patterns of lin-12 Expression During C. elegans Hermaphrodite Development

INVESTIGATIONS

Spatial and Temporal Patterns of lin-12 Expression During C. elegans Hermaphrodite Development

H. A. Wilkinson and IVA. Greenwald
Department of Biochemistry and Molecular Biophysics, Columbia University, College of Physicians and Surgeons, New York, New York 10032 Present address: Department of Cellular Biochemistry and Physiology, Merck Research Laboratories, Rahway, NJ 07065.

The lin-12 gene encodes a receptor that mediates certain cell-cell interactions during Caenorhabditis elegans development. We have examined the expression of a lin-12::lacZ reporter gene in individual cells during the development of C. elegans hermaphrodites. lin-12::lacZ is expressed in a discrete spatial and temporal pattern during development and the lin-12::lacZ reporter gene will provide a useful marker for other studies, particularly of somatic gonadal and vulval development. In general, the cells that express lin-12::lacZ correspond to cells whose fates are known to be altered in lin-12 mutants implying that restriction of lin-12 expression may be an important regulatory mechanism; the exceptions to this statement may reveal the cellular defects that underlie aspects of the lin-12 phenotype that have not been previously explained. For decisions that are not naturally variable, lin-12::lacZ expression does not appear to change before or upon commitment to a cell fate implying that in these cases posttranscriptional regulation of lin-12 activity may control cell fate specification.

This article has been cited by other articles:

X. Karp and I. Greenwald
Post-transcriptional regulation of the E/Daughterless ortholog HLH-2, negative feedback, and birth order bias during the AC/VU decision in C. elegans
Genes & Dev., December 15, 2003; 17(24): 3100 - 3111.
[Abstract] [Full Text] [PDF]

S. Jarriault and I. Greenwald
Suppressors of the egg-laying defective phenotype of sel-12 presenilin mutants implicate the CoREST corepressor complex in LIN-12/Notch signaling in C. elegans
Genes & Dev., October 15, 2002; 16(20): 2713 - 2728.
[Abstract] [Full Text] [PDF]

A. Meléndez and I. Greenwald
Caenorhabditis elegans lin-13, a Member of the LIN-35 Rb Class of Genes Involved in Vulval Development, Encodes a Protein With Zinc Fingers and an LXCXE Motif
Genetics, July 1, 2000; 155(3): 1127 - 1137.
[Abstract] [Full Text]

A. K. Corsi, S. A. Kostas, A. Fire, and M. Krause
Caenorhabditis elegans twist plays an essential role in non-striated muscle development
Development, May 15, 2000; 127(10): 2041 - 2051.
[Abstract] [PDF]

I. Greenwald
LIN-12/Notch signaling: lessons from worms and flies
Genes & Dev., June 15, 1998; 12(12): 1751 - 1762.
[Full Text]

L Nilsson, X Li, T Tiensuu, R Auty, I Greenwald, and S Tuck
Caenorhabditis elegans lin-25: cellular focus, protein expression and requirement for sur-2 during induction of vulval fates
Development, January 12, 1998; 125(23): 4809 - 4819.
[Abstract] [PDF]

D Levitan and I Greenwald
Effects of SEL-12 presenilin on LIN-12 localization and function in Caenorhabditis elegans
Development, January 9, 1998; 125(18): 3599 - 3606.
[Abstract] [PDF]

D Levitan and I Greenwald
LIN-12 protein expression and localization during vulval development in C. elegans
Development, January 8, 1998; 125(16): 3101 - 3109.
[Abstract] [PDF]

B. Harfe, C. Branda, M Krause, M. Stern, and A Fire
MyoD and the specification of muscle and non-muscle fates during postembryonic development of the C. elegans mesoderm
Development, January 7, 1998; 125(13): 2479 - 2488.
[Abstract] [PDF]

L Seugnet, P Simpson, and M Haenlin
Transcriptional regulation of Notch and Delta: requirement for neuroblast segregation in Drosophila
Development, January 5, 1997; 124(10): 2015 - 2025.
[Abstract] [PDF]

S Euling and V Ambros
Reversal of cell fate determination in Caenorhabditis elegans vulval development
Development, January 8, 1996; 122(8): 2507 - 2515.
[Abstract] [PDF]

				S. Jarriault and I. Greenwald Suppressors of the egg-laying defective phenotype of sel-12 presenilin mutants implicate the CoREST corepressor complex in LIN-12/Notch signaling in C. elegans Genes & Dev., October 15, 2002; 16(20): 2713 - 2728. [Abstract] [Full Text] [PDF]

				A. Meléndez and I. Greenwald Caenorhabditis elegans lin-13, a Member of the LIN-35 Rb Class of Genes Involved in Vulval Development, Encodes a Protein With Zinc Fingers and an LXCXE Motif Genetics, July 1, 2000; 155(3): 1127 - 1137. [Abstract] [Full Text]

				A. K. Corsi, S. A. Kostas, A. Fire, and M. Krause Caenorhabditis elegans twist plays an essential role in non-striated muscle development Development, May 15, 2000; 127(10): 2041 - 2051. [Abstract] [PDF]

				I. Greenwald LIN-12/Notch signaling: lessons from worms and flies Genes & Dev., June 15, 1998; 12(12): 1751 - 1762. [Full Text]

				L Nilsson, X Li, T Tiensuu, R Auty, I Greenwald, and S Tuck Caenorhabditis elegans lin-25: cellular focus, protein expression and requirement for sur-2 during induction of vulval fates Development, January 12, 1998; 125(23): 4809 - 4819. [Abstract] [PDF]

				D Levitan and I Greenwald Effects of SEL-12 presenilin on LIN-12 localization and function in Caenorhabditis elegans Development, January 9, 1998; 125(18): 3599 - 3606. [Abstract] [PDF]

				D Levitan and I Greenwald LIN-12 protein expression and localization during vulval development in C. elegans Development, January 8, 1998; 125(16): 3101 - 3109. [Abstract] [PDF]

				B. Harfe, C. Branda, M Krause, M. Stern, and A Fire MyoD and the specification of muscle and non-muscle fates during postembryonic development of the C. elegans mesoderm Development, January 7, 1998; 125(13): 2479 - 2488. [Abstract] [PDF]

				L Seugnet, P Simpson, and M Haenlin Transcriptional regulation of Notch and Delta: requirement for neuroblast segregation in Drosophila Development, January 5, 1997; 124(10): 2015 - 2025. [Abstract] [PDF]

				S Euling and V Ambros Reversal of cell fate determination in Caenorhabditis elegans vulval development Development, January 8, 1996; 122(8): 2507 - 2515. [Abstract] [PDF]