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Genetics, Vol 141, 453-464, Copyright © 1995
INVESTIGATIONS |
Extragenic Suppressors of nudC3, a Mutation That Blocks Nuclear Migration in Aspergillus nidulans
Y. H. Chiu and N. R. Morris
Department of Pharmacology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854
Nuclear migration plays an important role in the growth and development of many organisms including the filamentous fungus Aspergillus nidulans. We have cloned three genes from A. nidulans, nudA, nudC, and nudF, in which mutations affect nuclear migration. The nudA gene encodes the heavy chain of cytoplasmic dynein. The nudC gene encodes a 22-kD protein. The nudF gene was identified as an extracopy suppressor of the temperature sensitive (ts(-)) nudC3 mutation. The nudC3 mutation substantially decreases the intracellular concentration of the nudF protein at restrictive temperature. This is restored toward the normal level by an extra copy of nudF. To identify other genes whose products interact directly or indirectly with the NUDC protein, we have isolated a set of extragenic suppressors of the nudC3 temperature-sensitive mutation. Genetic analysis of 16 such extragenic suppressors showed them to represent nine different genes, designated sncA-sncI (for suppressor of nudC). sncA-sncH were either dominant or semidominant in diploids homozygous for nudC3 and heterozygous for the snc mutations. All of the suppressors reversed the ts(-) phenotype of nudC3 by restoring the intracellular concentration of the NUDF protein.
This article has been cited by other articles:
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  B. Hoffmann, W. Zuo, A. Liu, and N. R. Morris The LIS1-related Protein NUDF of Aspergillus nidulans and Its Interaction Partner NUDE Bind Directly to Specific Subunits of Dynein and Dynactin and to alpha - and gamma -Tubulin J. Biol. Chem., October 12, 2001; 276(42): 38877 - 38884. [Abstract] [Full Text] [PDF]
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 X. Xiang, A.H. Osmani, S.A. Osmani, C.H. Roghi, D.A. Willins, S. Beckwith, G. Goldman, Y. Chiu, M. Xin, B. Liu, et al. Analysis of Nuclear Migration in Aspergillus nidulans Cold Spring Harb Symp Quant Biol, January 1, 1995; 60(0): 813 - 819. [Abstract] [PDF]
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