- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Sinclair, D. A.
- Articles by Dawes, I. W.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Sinclair, D. A.
- Articles by Dawes, I. W.
Genetics, Vol 140, 1213-1222, Copyright © 1995
INVESTIGATIONS |
Genetics of the Synthesis of Serine from Glycine and the Utilization of Glycine as Sole Nitrogen Source by Saccharomyces cerevisiae
D. A. Sinclair and I. W. Dawes
School of Biochemistry and Molecular Genetics, The University of New South Wales, NSW, Australia 2052
Saccharomyces cerevisiae can grow on glycine as sole nitrogen source and can convert glycine to serine via the reaction catalyzed by the glycine decarboxylase multienzyme complex (GDC). Yeast strains with mutations in the single gene for lipoamide dehydrogenase (lpd1) lack GDC activity, as well as the other three 2-oxoacid dehydrogenases dependent on this enzyme. The LPD1 gene product is also required for cells to utilize glycine as sole nitrogen source. The effect of mutations in LPD1 (L-subunit of GDC), SER1 (synthesis of serine from 3-phosphoglycerate), ADE3 (cytoplasmic synthesis of one-carbon units for the serine synthesis from glycine), and all combinations of each has been determined. The results were used to devise methods for isolating mutants affected either in the generation of one-carbon units from glycine (via GDC) or subsequent steps in serine biosynthesis. The mutants fell into six complementation groups (gsd1-6 for defects in conversion of glycine to serine). Representatives from three complementation groups were also unable to grow on glycine as sole nitrogen source (gsd1-3). Assays of the rate of glycine uptake and decarboxylation have provided insights into the nature of the mutations.
This article has been cited by other articles:
 |
|
 |
|
  C. Gelling, I. W. Dawes, N. Richhardt, R. Lill, and U. Muhlenhoff Mitochondrial Iba57p Is Required for Fe/S Cluster Formation on Aconitase and Activation of Radical SAM Enzymes Mol. Cell. Biol., March 1, 2008; 28(5): 1851 - 1861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Albers, V. Laize, A. Blomberg, S. Hohmann, and L. Gustafsson Ser3p (Yer081wp) and Ser33p (Yil074cp) Are Phosphoglycerate Dehydrogenases in Saccharomyces cerevisiae J. Biol. Chem., March 14, 2003; 278(12): 10264 - 10272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. McCammon, C. B. Epstein, B. Przybyla-Zawislak, L. McAlister-Henn, and R. A. Butow Global Transcription Analysis of Krebs Tricarboxylic Acid Cycle Mutants Reveals an Alternating Pattern of Gene Expression and Effects on Hypoxic and Oxidative Genes Mol. Biol. Cell, March 1, 2003; 14(3): 958 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Dickinson, L. E. J. Salgado, and M. J. E. Hewlins The Catabolism of Amino Acids to Long Chain and Complex Alcohols in Saccharomyces cerevisiae J. Biol. Chem., February 28, 2003; 278(10): 8028 - 8034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Dickinson, S. J. Harrison, J. A. Dickinson, and M. J. E. Hewlins An Investigation of the Metabolism of Isoleucine to Active Amyl Alcohol in Saccharomyces cerevisiae J. Biol. Chem., April 6, 2000; 275(15): 10937 - 10942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-P. Hong, M. D. Piper, D. A. Sinclair, and I. W. Dawes Control of Expression of One-carbon Metabolism Genes of Saccharomyces cerevisiae Is Mediated by a Tetrahydrofolate-responsive Protein Binding to a Glycine Regulatory Region Including a Core 5'-CTTCTT-3' Motif J. Biol. Chem., April 9, 1999; 274(15): 10523 - 10532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Dickinson, S. J. Harrison, and M. J. E. Hewlins An Investigation of the Metabolism of Valine to Isobutyl Alcohol in Saccharomyces cerevisiae J. Biol. Chem., October 2, 1998; 273(40): 25751 - 25756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. G. ter Schure, M. T. Flikweert, J. P. van Dijken, J. T. Pronk, and C. T. Verrips Pyruvate Decarboxylase Catalyzes Decarboxylation of Branched-Chain 2-Oxo Acids but Is Not Essential for Fusel Alcohol Production by Saccharomyces cerevisiae Appl. Envir. Microbiol., April 1, 1998; 64(4): 1303 - 1307. [Abstract] [Full Text]
|
|
|
|
|
|
J. R. Dickinson, M. M. Lanterman, D. J. Danner, B. M. Pearson, P. Sanz, S. J. Harrison, and M. J. E. Hewlins A 13C Nuclear Magnetic Resonance Investigation of the Metabolism of Leucine to Isoamyl Alcohol in Saccharomyces cerevisiae J. Biol. Chem., October 24, 1997; 272(43): 26871 - 26878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Nagarajan and R. K. Storms Molecular Characterization of GCV3, the Saccharomyces cerevisiae Gene Coding for the Glycine Cleavage System Hydrogen Carrier Protein J. Biol. Chem., February 14, 1997; 272(7): 4444 - 4450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Piper, S.-P. Hong, G. E. Ball, and I. W. Dawes Regulation of the Balance of One-carbon Metabolism in Saccharomyces cerevisiae J. Biol. Chem., September 29, 2000; 275(40): 30987 - 30995. [Abstract] [Full Text] [PDF]
|
|