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Genetics, Vol 140, 1167-1174, Copyright © 1995
INVESTIGATIONS |
Host and {phi}X 174 Mutations Affecting the Morphogenesis or Stabilization of the 50S Complex, a Single-Stranded DNA Synthesizing Intermediate
M. C. Ekechukwu, D. J. Oberste and B. A. Fane
Department of Biological Sciences, University of Arkansas, Fayetteville, Arkansas 72701
The morphogenetic pathway of bacteriophage {phi}X 174 was investigated in rep mutant hosts that specifically block stage III single-stranded DNA synthesis. The defects conferred by the mutant rep protein most likely affect the formation or stabilization of the 50S complex, a single-stranded DNA synthesizing intermediate, which consists of a viral prohead and a DNA replicating intermediate (preinitiation complex). {phi}X 174 mutants, ogr(rep), which restore the ability to propagate in the mutant rep hosts, were isolated. The ogr(rep) mutations confer amino acid substitutions in the viral coat protein, a constituent of the prohead, and the viral A protein, a constituent of the preinitiation complex. Four of the six coat protein substitutions are localized on or near the twofold axis of symmetry in the atomic structure of the mature virion.
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