Specialization of B-Type Cyclins for Mitosis or Meiosis in S. cerevisiae

INVESTIGATIONS

Specialization of B-Type Cyclins for Mitosis or Meiosis in S. cerevisiae

C. Dahmann and B. Futcher
Present address: Reserach Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.

The CLB1, CLB2, and CLB3 genes encode B-type cyclins important for mitosis in Saccharomyces cerevisiae, while a fourth B-type cyclin gene, CLB4, has no clear role. The effects of homozygous clb mutations on meiosis were examined. Mutants homozygous for clb1 clb3, or for clb1 clb4, gave high levels of sporulation, but produced mainly two-spored asci instead of four-spored asci. The cells had completed meiosis I but not meiosis II, producing viable diploid ascospores. CLB1 and CLB4 seem to be much more important for meiosis than for mitosis and may play some special role in meiosis II. In contrast, CLB2 is important for mitosis but not meiosis. The level of Cdc28-Clb activity may be important in determining whether meiosis II will occur.

This article has been cited by other articles:

K. E. Sawarynski, N. A. Najor, A. C. Kepsel, and G. S. Brush
Sic1-induced DNA rereplication during meiosis
PNAS, January 6, 2009; 106(1): 232 - 237.
[Abstract] [Full Text] [PDF]

A. E. Diamond, J.-S. Park, I. Inoue, H. Tachikawa, and A. M. Neiman
The Anaphase Promoting Complex Targeting Subunit Ama1 Links Meiotic Exit to Cytokinesis during Sporulation in Saccharomyces cerevisiae
Mol. Biol. Cell, January 1, 2009; 20(1): 134 - 145.
[Abstract] [Full Text] [PDF]

B. M. Kiburz, A. Amon, and A. L. Marston
Shugoshin Promotes Sister Kinetochore Biorientation in Saccharomyces cerevisiae
Mol. Biol. Cell, March 1, 2008; 19(3): 1199 - 1209.
[Abstract] [Full Text] [PDF]

R. Strich, M. J. Mallory, M. Jarnik, and K. F. Cooper
Cyclin B-Cdk Activity Stimulates Meiotic Rereplication in Budding Yeast
Genetics, August 1, 2004; 167(4): 1621 - 1628.
[Abstract] [Full Text] [PDF]

K. R. Benjamin, C. Zhang, K. M. Shokat, and I. Herskowitz
Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2
Genes & Dev., June 15, 2003; 17(12): 1524 - 1539.
[Abstract] [Full Text] [PDF]

R. Sopko, S. Raithatha, and D. Stuart
Phosphorylation and Maximal Activity of Saccharomyces cerevisiae Meiosis-Specific Transcription Factor Ndt80 Is Dependent on Ime2
Mol. Cell. Biol., October 15, 2002; 22(20): 7024 - 7040.
[Abstract] [Full Text] [PDF]

J. Pak and J. Segall
Role of Ndt80, Sum1, and Swe1 as Targets of the Meiotic Recombination Checkpoint That Control Exit from Pachytene and Spore Formation in Saccharomyces cerevisiae
Mol. Cell. Biol., September 15, 2002; 22(18): 6430 - 6440.
[Abstract] [Full Text] [PDF]

M. A. Shonn, R. McCarroll, and A. W. Murray
Spo13 protects meiotic cohesin at centromeres in meiosis I
Genes & Dev., July 1, 2002; 16(13): 1659 - 1671.
[Abstract] [Full Text] [PDF]

M. Bolte, P. Steigemann, G. H. Braus, and S. Irniger
Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2
PNAS, April 2, 2002; 99(7): 4385 - 4390.
[Abstract] [Full Text] [PDF]

F. R. Cross, V. Archambault, M. Miller, and M. Klovstad
Testing a Mathematical Model of the Yeast Cell Cycle
Mol. Biol. Cell, January 1, 2002; 13(1): 52 - 70.
[Abstract] [Full Text] [PDF]

J. S. Choy, B. T. D. Tobe, J. H. Huh, and S. J. Kron
Yng2p-dependent NuA4 Histone H4 Acetylation Activity Is Required for Mitotic and Meiotic Progression
J. Biol. Chem., November 16, 2001; 276(47): 43653 - 43662.
[Abstract] [Full Text] [PDF]

S. Honey, B. L. Schneider, D. M. Schieltz, J. R. Yates, and B. Futcher
A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin-CDK complex
Nucleic Acids Res., February 15, 2001; 29(4): e24 - e24.
[Abstract] [Full Text] [PDF]

K. F. Cooper, M. J. Mallory, D. B. Egeland, M. Jarnik, and R. Strich
Ama1p is a meiosis-specific regulator of the anaphase promoting complex/cyclosome in yeast
PNAS, December 8, 2000; (2000) 250351297.
[Abstract] [Full Text]

K.-S. Tung, E.-J. E. Hong, and G. S. Roeder
The pachytene checkpoint prevents accumulation and phosphorylation of the meiosis-specific transcription factor Ndt80
PNAS, October 12, 2000; (2000) 220464597.
[Abstract] [Full Text]

B. Mai and L. Breeden
CLN1 and Its Repression by Xbp1 Are Important for Efficient Sporulation in Budding Yeast
Mol. Cell. Biol., January 15, 2000; 20(2): 478 - 487.
[Abstract] [Full Text]

M. E. Grether and I. Herskowitz
Genetic and Biochemical Characterization of the Yeast Spo12 Protein
Mol. Biol. Cell, November 1, 1999; 10(11): 3689 - 3703.
[Abstract] [Full Text]

J. M. Grushcow, T. M. Holzen, K. J. Park, T. Weinert, M. Lichten, and D. K. Bishop
Saccharomyces cerevisiae Checkpoint Genes MEC1, RAD17 and RAD24 Are Required for Normal Meiotic Recombination Partner Choice
Genetics, October 1, 1999; 153(2): 607 - 620.
[Abstract] [Full Text] [PDF]

M. D. Mendenhall and A. E. Hodge
Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae
Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1191 - 1243.
[Abstract] [Full Text] [PDF]

S. R. Hepworth, H. Friesen, and J. Segall
NDT80 and the Meiotic Recombination Checkpoint Regulate Expression of Middle Sporulation-Specific Genes in Saccharomyces cerevisiae
Mol. Cell. Biol., October 1, 1998; 18(10): 5750 - 5761.
[Abstract] [Full Text]

D. Stuart and C. Wittenberg
CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint
Genes & Dev., September 1, 1998; 12(17): 2698 - 2710.
[Abstract] [Full Text]

M. Bolte, P. Steigemann, G. H. Braus, and S. Irniger
Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2
PNAS, April 2, 2002; 99(7): 4385 - 4390.
[Abstract] [Full Text] [PDF]

K.-S. Tung, E.-J. E. Hong, and G. S. Roeder
The pachytene checkpoint prevents accumulation and phosphorylation of the meiosis-specific transcription factor Ndt80
PNAS, October 24, 2000; 97(22): 12187 - 12192.
[Abstract] [Full Text] [PDF]

K. F. Cooper, M. J. Mallory, D. B. Egeland, M. Jarnik, and R. Strich
Ama1p is a meiosis-specific regulator of the anaphase promoting complex/cyclosome in yeast
PNAS, December 19, 2000; 97(26): 14548 - 14553.
[Abstract] [Full Text] [PDF]

				A. E. Diamond, J.-S. Park, I. Inoue, H. Tachikawa, and A. M. Neiman The Anaphase Promoting Complex Targeting Subunit Ama1 Links Meiotic Exit to Cytokinesis during Sporulation in Saccharomyces cerevisiae Mol. Biol. Cell, January 1, 2009; 20(1): 134 - 145. [Abstract] [Full Text] [PDF]

				B. M. Kiburz, A. Amon, and A. L. Marston Shugoshin Promotes Sister Kinetochore Biorientation in Saccharomyces cerevisiae Mol. Biol. Cell, March 1, 2008; 19(3): 1199 - 1209. [Abstract] [Full Text] [PDF]

				R. Strich, M. J. Mallory, M. Jarnik, and K. F. Cooper Cyclin B-Cdk Activity Stimulates Meiotic Rereplication in Budding Yeast Genetics, August 1, 2004; 167(4): 1621 - 1628. [Abstract] [Full Text] [PDF]

				K. R. Benjamin, C. Zhang, K. M. Shokat, and I. Herskowitz Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2 Genes & Dev., June 15, 2003; 17(12): 1524 - 1539. [Abstract] [Full Text] [PDF]

				R. Sopko, S. Raithatha, and D. Stuart Phosphorylation and Maximal Activity of Saccharomyces cerevisiae Meiosis-Specific Transcription Factor Ndt80 Is Dependent on Ime2 Mol. Cell. Biol., October 15, 2002; 22(20): 7024 - 7040. [Abstract] [Full Text] [PDF]

				J. Pak and J. Segall Role of Ndt80, Sum1, and Swe1 as Targets of the Meiotic Recombination Checkpoint That Control Exit from Pachytene and Spore Formation in Saccharomyces cerevisiae Mol. Cell. Biol., September 15, 2002; 22(18): 6430 - 6440. [Abstract] [Full Text] [PDF]

				M. A. Shonn, R. McCarroll, and A. W. Murray Spo13 protects meiotic cohesin at centromeres in meiosis I Genes & Dev., July 1, 2002; 16(13): 1659 - 1671. [Abstract] [Full Text] [PDF]

				M. Bolte, P. Steigemann, G. H. Braus, and S. Irniger Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2 PNAS, April 2, 2002; 99(7): 4385 - 4390. [Abstract] [Full Text] [PDF]

				F. R. Cross, V. Archambault, M. Miller, and M. Klovstad Testing a Mathematical Model of the Yeast Cell Cycle Mol. Biol. Cell, January 1, 2002; 13(1): 52 - 70. [Abstract] [Full Text] [PDF]

				J. S. Choy, B. T. D. Tobe, J. H. Huh, and S. J. Kron Yng2p-dependent NuA4 Histone H4 Acetylation Activity Is Required for Mitotic and Meiotic Progression J. Biol. Chem., November 16, 2001; 276(47): 43653 - 43662. [Abstract] [Full Text] [PDF]

				S. Honey, B. L. Schneider, D. M. Schieltz, J. R. Yates, and B. Futcher A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin-CDK complex Nucleic Acids Res., February 15, 2001; 29(4): e24 - e24. [Abstract] [Full Text] [PDF]

				K. F. Cooper, M. J. Mallory, D. B. Egeland, M. Jarnik, and R. Strich Ama1p is a meiosis-specific regulator of the anaphase promoting complex/cyclosome in yeast PNAS, December 8, 2000; (2000) 250351297. [Abstract] [Full Text]

				K.-S. Tung, E.-J. E. Hong, and G. S. Roeder The pachytene checkpoint prevents accumulation and phosphorylation of the meiosis-specific transcription factor Ndt80 PNAS, October 12, 2000; (2000) 220464597. [Abstract] [Full Text]

				B. Mai and L. Breeden CLN1 and Its Repression by Xbp1 Are Important for Efficient Sporulation in Budding Yeast Mol. Cell. Biol., January 15, 2000; 20(2): 478 - 487. [Abstract] [Full Text]

				M. E. Grether and I. Herskowitz Genetic and Biochemical Characterization of the Yeast Spo12 Protein Mol. Biol. Cell, November 1, 1999; 10(11): 3689 - 3703. [Abstract] [Full Text]

				J. M. Grushcow, T. M. Holzen, K. J. Park, T. Weinert, M. Lichten, and D. K. Bishop Saccharomyces cerevisiae Checkpoint Genes MEC1, RAD17 and RAD24 Are Required for Normal Meiotic Recombination Partner Choice Genetics, October 1, 1999; 153(2): 607 - 620. [Abstract] [Full Text] [PDF]

				M. D. Mendenhall and A. E. Hodge Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1191 - 1243. [Abstract] [Full Text] [PDF]

				S. R. Hepworth, H. Friesen, and J. Segall NDT80 and the Meiotic Recombination Checkpoint Regulate Expression of Middle Sporulation-Specific Genes in Saccharomyces cerevisiae Mol. Cell. Biol., October 1, 1998; 18(10): 5750 - 5761. [Abstract] [Full Text]

				D. Stuart and C. Wittenberg CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint Genes & Dev., September 1, 1998; 12(17): 2698 - 2710. [Abstract] [Full Text]