- THIS ARTICLE
- Full Text (PDF)
- A corrigendum has been published
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Fujitani, Y.
- Articles by Kobayashi, I.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Fujitani, Y.
- Articles by Kobayashi, I.
Genetics, Vol 140, 797-809, Copyright © 1995
INVESTIGATIONS |
Dependence of Frequency of Homologous Recombination on the Homology Length
Y. Fujitani, K. Yamamoto and I. Kobayashi
Department of Physics, Faculty of Science, University of Tokyo, Tokyo 113, Japan.
The frequency of homologous recombination is believed to be a linear function of the length (N bp) of homology between DNAs. Here, the N intercept is believed to be determined by a threshold length below which some physical constraint is effective. In the mammalian gene targeting systems, however, the frequency depends more steeply than linearly on the homology length. To explain both the linear dependence and the steeper dependence, we propose a model where the branch point of a reaction intermediate is assumed to ``walk randomly'' along the homologous region until it is processed. The intermediate is assumed to be destroyed if the branch point ever reaches either end of the homology. In this model, the length dependence is governed by a parameter, h, which is defined as efficiency of processing of the intermediate and reflects unlikelihood of the destruction at either end of the homology. We find that the frequency is proportional to N(3) for smaller N and is a linear function of N for larger N. Where the shift from the N(3) dependence to the linear dependence takes place is determined by the parameter h. The range of N showing the N(3) dependence becomes narrower as h becomes larger. The dependence steeper than linear dependence, which is observed not only in the mammalian gene targeting system but also in bacteriophage T4, Escherichia coli and yeast systems, agrees well with the predicted N(3) dependence. The N intercept is determined not by physical (or structural) constraints but only by the parameter h in this model.
This article has been cited by other articles:
 |
|
 |
|
  T. Tsuru and I. Kobayashi Multiple Genome Comparison within a Bacterial Species Reveals a Unit of Evolution Spanning Two Adjacent Genes in a Tandem Paralog Cluster Mol. Biol. Evol., November 1, 2008; 25(11): 2457 - 2473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tsuru, M. Kawai, Y. Mizutani-Ui, I. Uchiyama, and I. Kobayashi Evolution of Paralogous Genes: Reconstruction of Genome Rearrangements Through Comparison of Multiple Genomes Within Staphylococcus aureus Mol. Biol. Evol., June 1, 2006; 23(6): 1269 - 1285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Lin, C. Zhang, and Y. Rikihisa Analysis of Involvement of the RecF Pathway in p44 Recombination in Anaplasma phagocytophilum and in Escherichia coli by Using a Plasmid Carrying the p44 Expression and p44 Donor Loci Infect. Immun., April 1, 2006; 74(4): 2052 - 2062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Di Primio, A. Galli, T. Cervelli, M. Zoppe, and G. Rainaldi Potentiation of gene targeting in human cells by expression of Saccharomyces cerevisiae Rad52 Nucleic Acids Res., August 16, 2005; 33(14): 4639 - 4648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Hansen, A. C. Dill, and D. W. Grogan Conjugational Genetic Exchange in the Hyperthermophilic Archaeon Sulfolobus acidocaldarius: Intragenic Recombination with Minimal Dependence on Marker Separation J. Bacteriol., January 15, 2005; 187(2): 805 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. H. Lu, J. T. Books, R. M. Kaufman, and T. J. Ley Long targeting arms do not increase the efficiency of homologous recombination in the {beta}-globin locus of murine embryonic stem cells Blood, August 15, 2003; 102(4): 1531 - 1533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Pluciennik, R. R. Iyer, M. Napierala, J. E. Larson, M. Filutowicz, and R. D. Wells Long CTG{middle dot}CAG Repeats from Myotonic Dystrophy Are Preferred Sites for Intermolecular Recombination J. Biol. Chem., September 6, 2002; 277(37): 34074 - 34086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Fujitani, S. Mori, and I. Kobayashi A Reaction-Diffusion Model for Interference in Meiotic Crossing Over Genetics, May 1, 2002; 161(1): 365 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Fujitani and I. Kobayashi Effect of DNA Sequence Divergence on Homologous Recombination as Analyzed by a Random-Walk Model Genetics, December 1, 1999; 153(4): 1973 - 1988. [Abstract] [Full Text]
|
|
|
|
|
|
T. A. Kavanagh, N. D. Thanh, N. T. Lao, N. McGrath, S. O. Peter, E. M. Horváth, P. J. Dix, and P. Medgyesy Homeologous Plastid DNA Transformation in Tobacco Is Mediated by Multiple Recombination Events Genetics, July 1, 1999; 152(3): 1111 - 1122. [Abstract] [Full Text]
|
|
|
|
|
|
O. Inbar, B. Liefshitz, G. Bitan, and M. Kupiec The Relationship between Homology Length and Crossing Over during the Repair of a Broken Chromosome J. Biol. Chem., September 29, 2000; 275(40): 30833 - 30838. [Abstract] [Full Text] [PDF]
|
|