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Genetics, Vol 140, 615-627, Copyright © 1995
INVESTIGATIONS |
Developmental Genetical Analysis and Molecular Cloning of the abnormal oocyte Gene of Drosophila melanogaster
J. Tomkiel, L. Fanti, M. Berloco, L. Spinelli, J. W. Tamkun, B. T. Wakimoto and S. Pimpinelli
Department of Genetics, University of Washington, Seattle, Washington 98195
Studies of the abnormal oocyte (abo) gene of Drosophila melanogaster have previously been limited to the analysis of a single mutant allele, abnormal oocyte(1) (abo(1)). The abo(1) mutation causes a maternal-effect lethality that can be partially rescued zygotically by the abo(+) allele and by increasing the dosage of specific regions of heterochromatin denoted ABO. This report describes the properties of abo(2), a new P-element-induced allele that allowed us to reexamine the nature of maternal-effect defect. Comparisons of the phenotype of progeny of abo(1)/abo(1) and abo(1)/abo(2) females show that the preblastoderm lethality previously described as a component of the abo mutant maternal effect results from a recessive fertilization defect associated with the abo(1) chromosome. We demonstrate here that the abo-induced maternal effect lethality occurs predominately late in embryogenesis after cuticle deposition but before hatching. The phenocritical period for zygotic rescue by heterochromatin coincides with this period of late embryogenesis. We have used the abo(2) mutation to map and molecularly clone the gene. We show that the abo gene is located in the 32C cytogenetic interval and identify the putative abo transcript from mRNA isolated from adult females. Using germline transformation, we show that a 9-kb genomic fragment to which the transcript maps, partially fulfills requirement for maternal and zygotic abo(+) function.
This article has been cited by other articles:
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  M. Berloco, L. Fanti, A. Breiling, V. Orlando, and S. Pimpinelli The maternal effect gene, abnormal oocyte (abo), of Drosophila melanogaster encodes a specific negative regulator of histones PNAS, September 26, 2001; (2001) 211428798. [Abstract] [Full Text] [PDF]
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M. Berloco, L. Fanti, A. Breiling, V. Orlando, and S. Pimpinelli The maternal effect gene, abnormal oocyte (abo), of Drosophila melanogaster encodes a specific negative regulator of histones PNAS, October 9, 2001; 98(21): 12126 - 12131. [Abstract] [Full Text] [PDF]
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