- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Barczak, A. J.
- Articles by Last, R. L.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Barczak, A. J.
- Articles by Last, R. L.
Genetics, Vol 140, 303-313, Copyright © 1995
INVESTIGATIONS |
5-Fluoroindole Resistance Identifies Tryptophan Synthase Beta Subunit Mutants in Arabidopsis thaliana
A. J. Barczak, J. Zhao, K. D. Pruitt and R. L. Last
Plant Molecular Biology Program, Boyce Thompson Institute for Plant Research and Section of Genetics and Development, Cornell University, Ithaca, New York 14853-1801
A study of the biochemical genetics of the Arabidopsis thaliana tryptophan synthase beta subunit was initiated by characterization of mutants resistant to the inhibitor 5-fluoroindole. Thirteen recessive mutations were recovered that are allelic to trp2-1, a mutation in the more highly expressed of duplicate tryptophan synthase beta subunit genes (TSB1). Ten of these mutations (trp2-2 through trp2-11) cause a tryptophan requirement (auxotrophs), whereas three (trp2-100 through trp2-102) remain tryptophan prototrophs. The mutations cause a variety of changes in tryptophan synthase beta expression. For example, two mutations (trp2-5 and trp2-8) cause dramatically reduced accumulation of TSB mRNA and immunologically detectable protein, whereas trp2-10 is associated with increased mRNA and protein. A correlation exists between the quantity of mutant beta and wild-type alpha subunit levels in the trp2 mutant plants, suggesting that the synthesis of these proteins is coordinated or that the quantity or structure of the beta subunit influences the stability of the alpha protein. The level of immunologically detectable anthranilate synthase alpha subunit protein is increased in the trp2 mutants, suggesting the possibility of regulation of anthranilate synthase levels in response to tryptophan limitation.
This article has been cited by other articles:
 |
|
 |
|
  A. L. Van Eenennaam, K. Lincoln, T. P. Durrett, H. E. Valentin, C. K. Shewmaker, G. M. Thorne, J. Jiang, S. R. Baszis, C. K. Levering, E. D. Aasen, et al. Engineering Vitamin E Content: From Arabidopsis Mutant to Soy Oil PLANT CELL, December 1, 2003; 15(12): 3007 - 3019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. H. Miesak and G. M. Coruzzi Molecular and Physiological Analysis of Arabidopsis Mutants Defective in Cytosolic or Chloroplastic Aspartate Aminotransferase Plant Physiology, June 1, 2002; 129(2): 650 - 660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Brader, E. Tas, and E. T. Palva Jasmonate-Dependent Induction of Indole Glucosinolates in Arabidopsis by Culture Filtrates of the Nonspecific Pathogen Erwinia carotovora Plant Physiology, June 1, 2001; 126(2): 849 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Mou, Y. He, Y. Dai, X. Liu, and J. Li Deficiency in Fatty Acid Synthase Leads to Premature Cell Death and Dramatic Alterations in Plant Morphology PLANT CELL, March 1, 2000; 12(3): 405 - 418. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. L. Conklin, S. A. Saracco, S. R. Norris, and R. L. Last Identification of Ascorbic Acid-Deficient Arabidopsis thaliana Mutants Genetics, February 1, 2000; 154(2): 847 - 856. [Abstract] [Full Text]
|
|
|
|
|
|
A. L. Palombella and S. K. Dutcher Identification of the Gene Encoding the Tryptophan Synthase beta -Subunit from Chlamydomonas reinhardtii Plant Physiology, June 1, 1998; 117(2): 455 - 464. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Bender and G. R. Fink A Myb homologue, ATR1, activates tryptophan gene expression in Arabidopsis PNAS, May 12, 1998; 95(10): 5655 - 5660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. G. Landry, A. E. Stapleton, J. Lim, P. Hoffman, J. B. Hays, V. Walbot, and R. L. Last An Arabidopsis photolyase mutant is hypersensitive to ultraviolet-B radiation PNAS, January 7, 1997; 94(1): 328 - 332. [Abstract] [Full Text] [PDF]
|
|