Genetics, Vol 139, 1631-1647, Copyright © 1995

INVESTIGATIONS

A Physical Map of the X Chromosome of Drosophila melanogaster: Cosmid Contigs and Sequence Tagged Sites

E. Madueno, G. Papagiannakis, G. Rimmington, RDC. Saunders, C. Savakis, I. Siden-Kiamos, G. Skavdis, L. Spanos, J. Trenear, P. Adam, M. Ashburner, P. Benos, V. N. Bolshakov, D. Coulson, D. M. Glover, S. Herrmann, F. C. Kafatos, C. Louis, T. Majerus and J. Modolell
Centro de Biologia Molecular Severo Ochoa, CSIC, Universidad Autonoma de Madrid, 28049 Madrid, Spain

A physical map of the euchromatic X chromosome of Drosophila melanogaster has been constructed by assembling contiguous arrays of cosmids that were selected by screening a library with DNA isolated from microamplified chromosomal divisions. This map, consisting of 893 cosmids, covers ~64% of the euchromatic part of the chromosome. In addition, 568 sequence tagged sites (STS), in aggregate representing 120 kb of sequenced DNA, were derived from selected cosmids. Most of these STSs, spaced at an average distance of ~35 kb along the euchromatic region of the chromosome, represent DNA tags that can be used as entry points to the fruitfly genome. Furthermore, 42 genes have been placed on the physical map, either through the hybridization of specific probes to the cosmids or through the fact that they were represented among the STSs. These provide a link between the physical and the genetic maps of D. melanogaster. Nine novel genes have been tentatively identified in Drosophila on the basis of matches between STS sequences and sequences from other species.

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