- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Weiler, K. S.
- Articles by Broach, J. R.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Weiler, K. S.
- Articles by Broach, J. R.
Genetics, Vol 139, 1495-1510, Copyright © 1995
INVESTIGATIONS |
Mutations Affecting Donor Preference During Mating Type Interconversion in Saccharomyces cerevisiae
K. S. Weiler, L. Szeto and J. R. Broach
Present address: Department of Zoology, NJ-15, University of Washington, Seattle, WA 98195.
Homothallic strains of Saccharomyces cerevisiae can convert mating type from a to {alpha} or {alpha} to a as often as every generation, by replacing genetic information specifying one mating type at the expressor locus, MAT, with information specifying the opposite mating type. The cryptic mating type information that is copied and inserted at MAT is contained in either of two loci, HML or HMR. The particular locus selected as donor during mating type interconversion is regulated by the allele expressed at MAT. MATa cells usually select HML, and MAT{alpha} cells usually select HMR, a process referred to as donor preference. To identify factors required for donor preference, we isolated and characterized a number of mutants that frequently selected the nonpreferred donor locus during mating type interconversion. Many of these mutants were found to harbor chromosome rearrangements or mutations at MAT or HML that interfered with the switching process. However, one mutant carried a recessive allele of CHL1, a gene previously shown to be required for efficient chromosome segregation during mitosis. Homothallic strains of yeast containing a null allele of CHL1 exhibited almost random selection of the donor locus in a MATa background but were normal in their ability to select HMR in a MAT{alpha} background. Our results indicate that Chl1p participates in the process of donor selection and are consistent with a model in which Chl1p helps establish an intrinsic bias in donor preference.
This article has been cited by other articles:
 |
|
 |
|
  S. Laha, S. P. Das, S. Hajra, S. Sau, and P. Sinha The budding yeast protein Chl1p is required to preserve genome integrity upon DNA damage in S-phase Nucleic Acids Res., November 6, 2006; 34(20): 5880 - 5891. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Coic, K. Sun, C. Wu, and J. E. Haber Cell Cycle-Dependent Regulation of Saccharomyces cerevisiae Donor Preference during Mating-Type Switching by SBF (Swi4/Swi6) and Fkh1. Mol. Cell. Biol., July 1, 2006; 26(14): 5470 - 5480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ruan, J. L. Workman, and R. T. Simpson The DNA Repair Protein yKu80 Regulates the Function of Recombination Enhancer during Yeast Mating Type Switching Mol. Cell. Biol., October 1, 2005; 25(19): 8476 - 8485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Z. Dalgaard and S. Vengrova Selective Gene Expression in Multigene Families from Yeast to Mammals Sci. Signal., October 26, 2004; 2004(256): re17 - re17. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Houston, P. J. Simon, and J. R. Broach The Saccharomyces cerevisiae Recombination Enhancer Biases Recombination During Interchromosomal Mating-Type Switching but Not in Interchromosomal Homologous Recombination Genetics, March 1, 2004; 166(3): 1187 - 1197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. V. Skibbens Chl1p, a DNA Helicase-Like Protein in Budding Yeast, Functions in Sister-Chromatid Cohesion Genetics, January 1, 2004; 166(1): 33 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sun, E. Coic, Z. Zhou, P. Durrens, and J. E. Haber Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer Genes & Dev., August 15, 2002; 16(16): 2085 - 2096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Holloway CHL1 is a nuclear protein with an essential ATP binding site that exhibits a size-dependent effect on chromosome segregation Nucleic Acids Res., August 15, 2000; 28(16): 3056 - 3064. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Paques and J. E. Haber Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 349 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wu, K. Weiss, C. Yang, M. A. Harris, B.-K. Tye, C. S. Newlon, R. T. Simpson, and J. E. Haber Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching Genes & Dev., June 1, 1998; 12(11): 1726 - 1737. [Abstract] [Full Text]
|
|
|
|
|
|
L. Szeto, M. K. Fafalios, H. Zhong, A. K. Vershon, and J. R. Broach alpha 2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III Genes & Dev., August 1, 1997; 11(15): 1899 - 1911. [Abstract] [Full Text] [PDF]
|
|