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Genetics, Vol 139, 1469-1481, Copyright © 1995
INVESTIGATIONS |
Involvement of the Host Initiator Function dnaA in the Replication of Coliphage {lambda}
G. Wegrzyn, A. Wegrzyn, I. Konieczny, K. Bielawski, G. Konopa, M. Obuchowski, D. R. Helinski and K. Taylor
Department of Molecular Biology, University of Gdansk, PL-80822 Poland
We demonstrate that the initiation of coliphage {lambda} DNA replication is dependent on the host initiator function dnaA, provided that the lambdoid prophage Rac is absent. Presence of Rac compensated the absence of dnaA function, causing initiation of replication. In dnaAts rac(+) cells at 43{deg}, most of parental phage DNA molecules, after one round of theta replication, switched to a replication with features of the sigma mode and produced progeny at high yield. Initiation of replication of the {lambda}Pts1 mutant at 43{deg} was blocked by dnaA function; however, under dnaA(-)rac(+) conditions all parental phage DNA molecules, after one round of theta replication, switched to the sigma mode and produced progeny at high yield. Taking into account our recent finding that transcriptional activation of ori{lambda} seems to be dnaA-regulated (to be published elsewhere), we suggest that the DnaA-{lambda}Pts1 incompatibility occurs at the insertion of the ori{lambda}-bound {lambda}O-{lambda}P-DnaB preprimosome between the complementary {lambda} DNA strands. The role of Rac and the mechanism of the switch from theta to sigma mode of {lambda} phage DNA replication are discussed.
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