Linkage Map of the Honey Bee, Apis mellifera, Based on RAPD Markers

INVESTIGATIONS

Linkage Map of the Honey Bee, Apis mellifera, Based on RAPD Markers

G. J. Hunt and R. E. Page-Jr
Department of Entomology, University of California, Davis, California 95616

A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female bee. The X locus for sex determination and genes for black body color and malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be ~3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species.

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				Y. Yasukochi, L. A. Ashakumary, K. Baba, A. Yoshido, and K. Sahara A Second-Generation Integrated Map of the Silkworm Reveals Synteny and Conserved Gene Order Between Lepidopteran Insects Genetics, July 1, 2006; 173(3): 1319 - 1328. [Abstract] [Full Text] [PDF]

				O. Rueppell, S. B. C. Chandra, T. Pankiw, M. K. Fondrk, M. Beye, G. Hunt, and R. E. Page The Genetic Architecture of Sucrose Responsiveness in the Honeybee (Apis mellifera L.) Genetics, January 1, 2006; 172(1): 243 - 251. [Abstract] [Full Text] [PDF]

				O. Ruppell, T. Pankiw, and R. E. Page Jr. Pleiotropy, Epistasis and New QTL: The Genetic Architecture of Honey Bee Foraging Behavior J. Hered., November 1, 2004; 95(6): 481 - 491. [Abstract] [Full Text] [PDF]

				B. A. Pannebakker, L. W. Beukeboom, J. J. M. van Alphen, P. M. Brakefield, and B. J. Zwaan The Genetic Basis of Male Fertility in Relation to Haplodiploid Reproduction in Leptopilina clavipes (Hymenoptera: Figitidae) Genetics, September 1, 2004; 168(1): 341 - 349. [Abstract] [Full Text] [PDF]

				O. Rueppell, T. Pankiw, D. I. Nielsen, M. K. Fondrk, M. Beye, and R. E. Page Jr. The Genetic Architecture of the Behavioral Ontogeny of Foraging in Honeybee Workers Genetics, August 1, 2004; 167(4): 1767 - 1779. [Abstract] [Full Text] [PDF]

				E. Baudry, P. Kryger, M. Allsopp, N. Koeniger, D. Vautrin, F. Mougel, J.-M. Cornuet, and M. Solignac Whole-Genome Scan in Thelytokous-Laying Workers of the Cape Honeybee (Apis mellifera capensis): Central Fusion, Reduced Recombination Rates and Centromere Mapping Using Half-Tetrad Analysis Genetics, May 1, 2004; 167(1): 243 - 252. [Abstract] [Full Text] [PDF]

				M. Solignac, D. Vautrin, E. Baudry, F. Mougel, A. Loiseau, and J.-M. Cornuet A Microsatellite-Based Linkage Map of the Honeybee, Apis mellifera L. Genetics, May 1, 2004; 167(1): 253 - 262. [Abstract] [Full Text] [PDF]

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				C. W. Whitfield, M. R. Band, M. F. Bonaldo, C. G. Kumar, L. Liu, J. R. Pardinas, H. M. Robertson, M. B. Soares, and G. E. Robinson Annotated Expressed Sequence Tags and cDNA Microarrays for Studies of Brain and Behavior in the Honey Bee Genome Res., April 1, 2002; 12(4): 555 - 566. [Abstract] [Full Text] [PDF]

				R. E. Page Jr., J. Gadau, and M. Beye The Emergence of Hymenopteran Genetics Genetics, February 1, 2002; 160(2): 375 - 379. [Full Text] [PDF]

				D. J. Hawthorne AFLP-Based Genetic Linkage Map of the Colorado Potato Beetle Leptinotarsa decemlineata: Sex Chromosomes and a Pyrethroid-Resistance Candidate Gene Genetics, June 1, 2001; 158(2): 695 - 700. [Abstract] [Full Text] [PDF]

				L. M. Larraya, G. Pérez, E. Ritter, A. G. Pisabarro, and L. Ramírez Genetic Linkage Map of the Edible Basidiomycete Pleurotus ostreatus Appl. Envir. Microbiol., December 1, 2000; 66(12): 5290 - 5300. [Abstract] [Full Text]

				H. Laayouni, M. Santos, and A. Fontdevila Toward a Physical Map of Drosophila buzzatii: Use of Randomly Amplified Polymorphic DNA Polymorphisms and Sequence-Tagged Site Landmarks Genetics, December 1, 2000; 156(4): 1797 - 1816. [Abstract] [Full Text]

				D. K. Santra, M. Tekeoglu, M. Ratnaparkhe, W. J. Kaiser, and F. J. Muehlbauer Identification and Mapping of QTLs Conferring Resistance to Ascochyta Blight in Chickpea Crop Sci., November 1, 2000; 40(6): 1606 - 1612. [Abstract] [Full Text] [PDF]

				A. K. Holloway, M. R. Strand, W. C. Black , IV, and M. F. Antolin Linkage Analysis of Sex Determination in Bracon sp. Near hebetor (Hymenoptera: Braconidae) Genetics, January 1, 2000; 154(1): 205 - 212. [Abstract] [Full Text]

				M. Ohtsuka, S. Makino, K. Yoda, H. Wada, K. Naruse, H. Mitani, A. Shima, K. Ozato, M. Kimura, and H. Inoko Construction of a Linkage Map of the Medaka (Oryzias latipes) and Mapping of the Da Mutant Locus Defective in Dorsoventral Patterning Genome Res., December 1, 1999; 9(12): 1277 - 1287. [Abstract] [Full Text]

				M. Beye, G. J. Hunt, R. E. Page, M. K. Fondrk, L. Grohmann, and R. F. A. Moritz Unusually High Recombination Rate Detected in the Sex Locus Region of the Honey Bee (Apis mellifera) Genetics, December 1, 1999; 153(4): 1701 - 1708. [Abstract] [Full Text]

				J. Gadau, R. E. Page , Jr., and J. H. Werren Mapping of Hybrid Incompatibility Loci in Nasonia Genetics, December 1, 1999; 153(4): 1731 - 1741. [Abstract] [Full Text]

				R. W. Beeman and S. J. Brown RAPD-Based Genetic Linkage Maps of Tribolium castaneum Genetics, September 1, 1999; 153(1): 333 - 338. [Abstract] [Full Text]

				Y. Yasukochi A Dense Genetic Map of the Silkworm, Bombyx mori, Covering All Chromosomes Based on 1018 Molecular Markers Genetics, December 1, 1998; 150(4): 1513 - 1525. [Abstract] [Full Text]

				V. Laurent, E. Wajnberg, B. Mangin, T. Schiex, C. Gaspin, and F. Vanlerberghe-Masutti A Composite Genetic Map of the Parasitoid Wasp Trichogramma brassicae Based on RAPD Markers Genetics, September 1, 1998; 150(1): 275 - 282. [Abstract] [Full Text]

				G. J. Hunt, E. Guzman-Novoa, M. K. Fondrk, and R. E. Page , Jr. Quantitative Trait Loci for Honey Bee Stinging Behavior and Body Size Genetics, March 1, 1998; 148(3): 1203 - 1213. [Abstract] [Full Text] [PDF]