Multiple Elements and Auto-Repression Regulate Rox1, a Repressor of Hypoxic Genes in Saccharomyces cerevisiae

INVESTIGATIONS

Multiple Elements and Auto-Repression Regulate Rox1, a Repressor of Hypoxic Genes in Saccharomyces cerevisiae

J. Deckert, R. Perini, B. Balasubramanian and R. S. Zitomer
Department of Biological Sciences, The State University of New York at Albany, Albany, New York 12222

The ROX1 gene encodes a heme-induced repressor of hypoxic genes in yeast. Using RNA blot analysis and a ROX1/lacZ fusion construct that included the ROX1 upstream region and only the first codon, we discovered that Rox1 represses its own expression. Gel-retardation experiments indicated that Rox1 was capable of binding to its own upstream region. Overexpression of Rox1 from the inducible GAL1 promoter was found to be inhibitory to cell growth. Also, we found that, as reported previously, Hap1 is partially responsible for heme-induction of ROX1, but, in addition, it also may play a role in ROX1 repression in the absence of heme. There is a second repressor of anaerobic ROX1 expression that requires the general repressor Tup1/Ssn6 for its function.

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				K. W. Henry, J. T. Nickels, and T. D. Edlind ROX1 and ERG Regulation in Saccharomyces cerevisiae: Implications for Antifungal Susceptibility Eukaryot. Cell, December 1, 2002; 1(6): 1041 - 1044. [Abstract] [Full Text] [PDF]

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				R. A. Khalaf and R. S. Zitomer The DNA Binding Protein Rfg1 Is a Repressor of Filamentation in Candida albicans Genetics, April 1, 2001; 157(4): 1503 - 1512. [Abstract] [Full Text]

				N. E. Abramova, B. D. Cohen, O. Sertil, R. Kapoor, K. J. A. Davies, and C. V. Lowry Regulatory Mechanisms Controlling Expression of the DAN/TIR Mannoprotein Genes During Anaerobic Remodeling of the Cell Wall in Saccharomyces cerevisiae Genetics, March 1, 2001; 157(3): 1169 - 1177. [Abstract] [Full Text]

				B. D. Cohen, O. Sertil, N. E. Abramova, K. J. A. Davies, and C. V. Lowry Induction and repression of DAN1 and the family of anaerobic mannoprotein genes in Saccharomyces cerevisiae occurs through a complex array of regulatory sites Nucleic Acids Res., February 1, 2001; 29(3): 799 - 808. [Abstract] [Full Text] [PDF]

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				A. J. Kastaniotis, T. A. Mennella, C. Konrad, A. M. R. Torres, and R. S. Zitomer Roles of Transcription Factor Mot3 and Chromatin in Repression of the Hypoxic Gene ANB1 in Yeast Mol. Cell. Biol., October 1, 2000; 20(19): 7088 - 7098. [Abstract] [Full Text]

				M. A. Garcia-Gimeno and K. Struhl Aca1 and Aca2, ATF/CREB Activators in Saccharomyces cerevisiae, Are Important for Carbon Source Utilization but Not the Response to Stress Mol. Cell. Biol., June 15, 2000; 20(12): 4340 - 4349. [Abstract] [Full Text]

				B. R. Braun and A. D. Johnson TUP1, CPH1 and EFG1 Make Independent Contributions to Filamentation in Candida albicans Genetics, May 1, 2000; 155(1): 57 - 67. [Abstract] [Full Text]

				J. J. M. ter Linde, H. Liang, R. W. Davis, H. Y. Steensma, J. P. van Dijken, and J. T. Pronk Genome-Wide Transcriptional Analysis of Aerobic and Anaerobic Chemostat Cultures of Saccharomyces cerevisiae J. Bacteriol., December 15, 1999; 181(24): 7409 - 7413. [Abstract] [Full Text]

				J. Deckert, A. M. R. Torres, S. M. Hwang, A. J. Kastaniotis, and R. S. Zitomer The Anatomy of a Hypoxic Operator in Saccharomyces cerevisiae Genetics, December 1, 1998; 150(4): 1429 - 1441. [Abstract] [Full Text]

				Y. Chantrel, M. Gaisne, C. Lions, and J. Verdiere The Transcriptional Regulator Hap1p (Cyp1p) Is Essential for Anaerobic or Heme-Deficient Growth of Saccharomyces cerevisiae: Genetic and Molecular Characterization of an Extragenic Suppressor that Encodes a WD Repeat Protein Genetics, February 1, 1998; 148(2): 559 - 570. [Abstract] [Full Text] [PDF]

				P. M. Carrico and R. S. Zitomer Mutational Analysis of the Tup1 General Repressor of Yeast Genetics, February 1, 1998; 148(2): 637 - 644. [Abstract] [Full Text] [PDF]

				P. V. Burke, D. C. Raitt, L. A. Allen, E. A. Kellogg, and R. O. Poyton Effects of Oxygen Concentration on the Expression of Cytochrome c and Cytochrome c Oxidase Genes in Yeast J. Biol. Chem., June 6, 1997; 272(23): 14705 - 14712. [Abstract] [Full Text] [PDF]

				J.-M. Amillet, N. Buisson, and R. Labbe-Bois Characterization of an Upstream Activation Sequence and Two Rox1p-responsive Sites Controlling the Induction of the Yeast HEM13 Gene by Oxygen and Heme Deficiency J. Biol. Chem., October 4, 1996; 271(40): 24425 - 24432. [Abstract] [Full Text] [PDF]

				M. J. Vasconcelles, Y. Jiang, K. McDaid, L. Gilooly, S. Wretzel, D. L. Porter, C. E. Martin, and M. A. Goldberg Identification and Characterization of a Low Oxygen Response Element Involved in the Hypoxic Induction of a Family of Saccharomyces cerevisiae Genes. IMPLICATIONS FOR THE CONSERVATION OF OXYGEN SENSING IN EUKARYOTES J. Biol. Chem., April 20, 2001; 276(17): 14374 - 14384. [Abstract] [Full Text] [PDF]