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Genetics, Vol 139, 1107-1121, Copyright © 1995
INVESTIGATIONS |
On the Clustered Exchanges of the RecBCD Pathway Operating on Phage {lambda}
F. W. Stahl, C. E. Shurvinton, L. C. Thomason, S. Hill and M. M. Stahl
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403-1229
Lytic cycle crosses of Red(-) Gam(-) phage {lambda} were conducted in rec(+) Escherichia coli carrying one or another plasmid with homology to {lambda}. {lambda} X {lambda} recombinants and {lambda} X plasmid recombinants were formed by RecBCD-mediated recombination. We showed previously that the act of recombining with a plasmid alters the disposition of selected {lambda} X {lambda} exchanges. This work reports that the relationships between the {lambda} X plasmid and the {lambda} X {lambda} exchanges is unaltered by the removal from one {lambda} parent of the homology shared with the plasmid. This result supports our view that a reciprocal exchange, allowing for cointegrate formation, is associated with but mechanistically separable from a (presumably) nonreciprocal {lambda} X {lambda} exchange. The nature of this relationship is independent of {lambda}'s Rap function, which is shown to alter the ratio of cointegrate formation (splices) to marker pick-up (patches) in {lambda} X plasmid recombination mediated by the RecBCD pathway.
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