Genetics, Vol 139, 1091-1097, Copyright © 1995

INVESTIGATIONS

Multilocus Analysis for Gene-Centromere Mapping Using First Polar Bodies and Secondary Oocytes

Y. Da, V. L. Jarrell, T. Wang, R. L. Fernando, M. B. Wheeler and H. A. Lewin
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801

Polar body and oocyte typing is a new technique for gene-centromere mapping and for generating female linkage maps. A maximum likelihood approach is presented for ordering multiple markers relative to the centromere and for estimating recombination frequencies between markers and between the centromere and marker loci. Three marker-centromere orders are possible for each pair of markers: two orders when the centromere flanks the two markers and one order when the centromere is flanked by the two markers. For each possible order, the likelihood was expressed as a function of recombination frequencies for two adjacent intervals. LOD score for recombination frequency between markers or between the centromere and a marker locus was derived based on the likelihood for each gene-centromere order. The methods developed herein provide a general solution to the problem of multilocus genecentromere mapping that involves all theoretical crossover possibilities, including four-strand double crossovers.

This article has been cited by other articles:

				H. Zhao and T. P. Speed Statistical Analysis of Half-Tetrads Genetics, September 1, 1998; 150(1): 473 - 485. [Abstract] [Full Text]