Genetics, Vol 139, 95-107, Copyright © 1995

INVESTIGATIONS

Overexpression of SIS2, Which Contains an Extremely Acidic Region, Increases the Expression of SWI4, CLN1 and CLN2 in sit4 mutants

C. J. Di-Como, R. Bose and K. T. Arndt
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724-2212 and Graduate Program in Genetics, State University of New York, Stony Brook, New York 11794

The Saccharomyces cerevisiae SIS2 gene was identified by its ability, when present on a high copy number plasmid, to increase dramatically the growth rate of sit4 mutants. SIT4 encodes a type 2A-related protein phosphatase that is required in late G1 for normal G1 cyclin expression and for bud initiation. Overexpression of SIS2, which contains an extremely acidic carboxyl terminal region, stimulated the rate of CLN1, CLN2, SWI4 and CLB5 expression in sit4 mutants. Also, overexpression of SIS2 in a CLN1 cln2 cln3 strain stimulated the growth rate and the rate of CLN1 and CLB5 RNA accumulation during late G1. The SIS2 protein fractionated with nuclei and was released from the nuclear fraction by treatment with either DNase I or micrococcal nuclease, but not by RNase A. This result, combined with the finding that overexpression of SIS2 is extremely toxic to a strain containing lower than normal levels of histones H2A and H2B, suggests that SIS2 might function to stimulate transcription via an interaction with chromatin.

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