- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Clark, A. G.
- Articles by Hulleberg, T.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Clark, A. G.
- Articles by Hulleberg, T.
Genetics, Vol 139, 337-348, Copyright © 1995
INVESTIGATIONS |
P-Element-Induced Variation in Metabolic Regulation in Drosophila
A. G. Clark, L. Wang and T. Hulleberg
Department of Biology, Pennsylvania State University, University Park, Pensylvania 16802
Movement of transposable elements has been demonstrated to be a cause of genetic variation that is relevant to quantitative characters in Drosophila. Here a particular class of P-element-induced variation known to be mediated through changes in expression of targeted enzyme-encoding genes is examined. Balancer chromosomes and the ``jumpstarter'' modified P-element were used to construct 124 second-chromosome and 139 third-chromosome lines of Drosophila melanogaster bearing unique stable P-element insertions in a common genetic background. Lines that were homozygous for second-chromosome P-element insertions were significantly more heterogeneous than control lines in 10 of 16 characters, whereas third-chromosome insertion lines were heterogeneous in 11 of the 16 traits. The average mutational variance per insertion relative to environmental variance (V(m|1)/V(e)) was 5.7 X 10(-2), and estimates varied widely across characters. The distributions of mutational effects tended to be skewed, with a longer tail toward high enzyme activities. Mutational effects deviated from a normal distribution in 15 of the 16 traits and significant outlier lines were found in both a positive and negative direction in several characters. Pleiotropic effects of single P-element insertions were quantified by correlation, and, after correcting for simultaneous tests, of the 91 correlations, 37 were significant at the 5% level. The pattern of pleiotropic effects deviated both from the equilibrium genetic correlations quantified in a previous study and from the correlations of mutational effects in a mutation-accumulation experiment, suggesting that multiple forces are at play that shape extant variation.
This article has been cited by other articles:
 |
|
 |
|
  S. T. Harbison and A. Sehgal Quantitative Genetic Analysis of Sleep in Drosophila melanogaster Genetics, April 1, 2008; 178(4): 2341 - 2360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Weber, N. Johnson, D. Champlin, and A. Patty Many P-Element Insertions Affect Wing Shape in Drosophila melanogaster Genetics, March 1, 2005; 169(3): 1461 - 1475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Montooth, J. H. Marden, and A. G. Clark Mapping Determinants of Variation in Energy Metabolism, Respiration and Flight in Drosophila Genetics, October 1, 2003; 165(2): 623 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Bhadra, M. P. Bhadra, and J. A. Birchler Interactions Among Dosage-Dependent Trans-Acting Modifiers of Gene Expression and Position-Effect Variegation in Drosophila Genetics, September 1, 1998; 150(1): 251 - 263. [Abstract] [Full Text]
|
|
|
|
|
|
T. Mitchell-Olds and D. Pedersen The Molecular Basis of Quantitative Genetic Variation in Central and Secondary Metabolism in Arabidopsis Genetics, June 1, 1998; 149(2): 739 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Kidwell and D. Lisch Transposable elements as sources of variation in animals and plants PNAS, July 22, 1997; 94(15): 7704 - 7711. [Abstract] [Full Text] [PDF]
|
|