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Genetics, Vol 139, 327-335, Copyright © 1995
INVESTIGATIONS |
Aberrant Splicing and Transcription Termination caused by P Element Insertion into the Intron of a Drosophila Gene
H. Horowitz and C. A. Berg
Department of Genetics, University of Washington, Seattle, Washington 98195
Insertional mutagenesis screens using the P[lacZ, rosy(+)] (PZ) transposable element have provided thousands of mutant lines for analyzing genes of varied function in the fruitfly, Drosophila melanogaster. As has been observed with other P elements, many of the PZ-induced mutations result from insertion of the P element into the promoter or 5' untranslated regions of the affected gene. We document here a novel mechanism for mutagenesis by this element. We show that sequences present within the element direct aberrant splicing and termination events that produce a mRNA composed of 5' sequences from the mutated gene (in this case, pipsqueak) and 3' sequences from within the P[lacZ, rosy(+)] element. These truncated RNAs could yield proteins with dominant mutant effects.
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