Mutations that Alter the Timing and Pattern of cubitus interruptus Gene Expression in Drosophila melanogaster

INVESTIGATIONS

Mutations that Alter the Timing and Pattern of cubitus interruptus Gene Expression in Drosophila melanogaster

D. C. Slusarski, C. K. Motzny and R. Holmgren
Present address: Department of Biology, Johns Hopkins University, Baltimore, MD 21218-2685. Co-first authors.

The cubitus interruptus (ci) gene is a member of the Drosophila segment polarity gene family and encodes a protein with a zinc finger domain homologous to the vertebrate Gli genes and the nematode tra-1 gene. Three classes of existing mutations in the ci locus alter the regulation of ci expression and can be used to examine ci function during development. The first class of ci mutations causes interruptions in wing veins four and five due to inappropriate expression of the ci product in the posterior compartment of imaginal discs. The second class of mutations eliminates ci protein early in embryogenesis and causes the deletion of structures that are derived from the region including and adjacent to the engrailed expressing cells. The third class of mutations eliminates ci protein later in embryogenesis and blocks the formation of the ventral naked cuticle. The loss of ci expression at these two different stages in embryonic development correlates with the subsequent elimination of wingless expression. Adults heterozygous for the unique ci(Ce) mutation have deletions between wing veins three and four. A similar wing defect is present in animals mutant for the segment polarity gene fused that encodes a putative serine/threonine kinase. In ci(Ce)/+ and fused mutants, the deletions between wing veins three and four correlate with increased ci protein levels in the anterior compartment. Thus, proper regulation of both the ci mRNA and protein appears to be critical for normal Drosophila development.

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				C. W. Larsen, E. Hirst, C. Alexandre, and J.-P. Vincent Segment boundary formation in Drosophila embryos Development, December 1, 2003; 130(23): 5625 - 5635. [Abstract] [Full Text] [PDF]

				W. Fu and N. E. Baker Deciphering synergistic and redundant roles of Hedgehog, Decapentaplegic and Delta that drive the wave of differentiation in Drosophila eye development Development, November 1, 2003; 130(21): 5229 - 5239. [Abstract] [Full Text] [PDF]

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				J. Bai and D. Montell Eyes Absent, a key repressor of polar cell fate during Drosophila oogenesis Development, January 12, 2002; 129(23): 5377 - 5388. [Abstract] [Full Text] [PDF]

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				S. Blair and A Ralston Smoothened-mediated Hedgehog signalling is required for the maintenance of the anterior-posterior lineage restriction in the developing wing of Drosophila Development, January 10, 1997; 124(20): 4053 - 4063. [Abstract] [PDF]

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				J Hepker, Q. Wang, C. Motzny, R Holmgren, and T. Orenic Drosophila cubitus interruptus forms a negative feedback loop with patched and regulates expression of Hedgehog target genes Development, January 1, 1997; 124(2): 549 - 558. [Abstract] [PDF]

				Y. Tanabe and T. M. Jessell Diversity and Pattern in the Developing Spinal Cord Science, November 15, 1996; 274(5290): 1115 - 1123. [Abstract] [Full Text]

				J L Gomez-Skarmeta and J Modolell araucan and caupolican provide a link between compartment subdivisions and patterning of sensory organs and veins in the Drosophila wing. Genes & Dev., November 15, 1996; 10(22): 2935 - 2945. [Abstract] [PDF]