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Genetics, Vol 138, 1207-1238, Copyright © 1994
INVESTIGATIONS |
A Genetic Linkage Map of the Mouse Using Restriction Landmark Genomic Scanning (RLGS)
Y. Hayashizaki, S. Hirotsune, Y. Okazaki, H. Shibata, A. Akasako, M. Muramatsu, J. Kawai, T. Hirasawa, S. Watanabe, T. Shiroishi, K. Moriwaki, B. A. Taylor, Y. Matsuda, R. W. Elliott, K. F. Manly and V. M. Chapman
Gene Bank, The Institute of Physical and Chemical Research (RIKEN), Tsukuba Science City, Ibaraki, 305 Japan, Department of Bioscience, National Cardiovascular Center Research Institute, Osaka, 565 Japan
We have developed a multiplex method of genome analysis, restriction landmark genomic scanning (RLGS) that has been used to construct genetic maps in mice. Restriction landmarks are end-labeled restriction fragments of genomic DNA that are separated by using high resolution, two-dimensional gel electrophoresis identifying as many as two thousand landmark loci in a single gel. Variation for several hundred of these loci has been identified between laboratory strains and between these strains and Mus spretus. The segregation of more than 1100 RLGS loci has been analyxed in recombinant inbred (RI) strains and in two separate interspecific genetic crosses. Genetic maps have been derived that link 1045 RLGS loci to reference loci on all of the autosomes and the X chromosome of the mouse genome. The RLGS method can be applied to genome analysis in many different organisms to identify genomic loci because it used end-labeling of restriction landmarks rather than probe hybridization. Different combinations of restriction enzymes yield different sets of RLGS loci providing expanded power for genetic mapping.
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