Genetics, Vol 138, 609-619, Copyright © 1994

INVESTIGATIONS

Protein-Protein Interactions in the Yeast Pheromone Response Pathway: Ste5p Interacts With All Members of the MAP Kinase Cascade

J. A. Printen and G. F. Sprague-Jr.
Institute of Molecular Biology and Departments of Chemistry, University of Oregon, Eugene, Oregon 97403-1229

We have used the two-hybrid system of Fields and Song to identify protein-protein interactions that occur in the pheromone response pathway of the yeast Saccharomyces cerevisiae. Pathway components Ste4p, Ste5p, Ste7p, Ste11p, Ste12p, Ste20p, Fus3p and Kss1p were tested in all pairwise combinations. All of the interactions we detected involved at least one member of the MAP kinase cascade that is a central element of the response pathway. Ste5p, a protein of unknown biochemical function, interacted with protein kinases that operate at each step of the MAP kinase cascade, specifically with Ste11p (an MEKK), Ste7p (an MEK), and Fus3p (a MAP kinase). This finding suggests that one role of Ste5p is to serve as a scaffold to facilitate interactions among members of the kinase cascade. In this role as facilitator, Ste5p may make both signal propagation and signal attenuation more efficient. Ste5p may also help minimize cross-talk with other MAP kinase cascades and thus ensure the integrity of the pheromone response pathway. We also found that both Ste11p and Ste7p interact with Fus3p and Kss1p. Finally, we detected an interaction between one of the MAP kinases, Kss1p, and a presumptive target, the transcription factor Ste12p. We failed to detect interactions of Ste4p or Ste20p with any other component of the response pathway.

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				S. A. Burchett, A. Scott, B. Errede, and H. G. Dohlman Identification of Novel Pheromone-response Regulators through Systematic Overexpression of 120 Protein Kinases in Yeast J. Biol. Chem., July 6, 2001; 276(28): 26472 - 26478. [Abstract] [Full Text] [PDF]

				H. Zhang, Z. Zhu, G. Vidanes, D. Mbangkollo, Y. Liu, and W. Siede Characterization of DNA Damage-stimulated Self-interaction of Saccharomyces cerevisiae Checkpoint Protein Rad17p J. Biol. Chem., July 6, 2001; 276(28): 26715 - 26723. [Abstract] [Full Text] [PDF]