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Genetics, Vol 138, 283-287, Copyright © 1994
INVESTIGATIONS |
Mapping of DBR1 and YPK1 Suggests a Major Revision of the Genetic Map of the Left Arm of Saccharomyces cerevisiae Chromosome XI
G. Simchen, K. B. Chapman, E. Caputo, K. Nam, L. Riles, D. E. Levin and J. D. Boeke
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore Maryland 21205, Department of Genetics, Hebrew University, Jerusalem, Israel
The Saccharomyces cerevisiae dbr1 mutation has been mapped on the left arm of chromosome XI. XIL is a chromosome arm that was until now rather sparsely populated with accurately mapped markers. On the basis of physical data, the overall order of markers is inverted relative to the existing genetic map of XI. We present tetrad analyses using a variety of markers on XI that indicate that the existing genetic map of XIL should be inverted, at least for the strains in which our mapping was carried out, and probably for other S. cerevisiae strains.