Three Additional Linkage Groups That Repress Transcription and Meiotic Recombination in the Mating-Type Region of Schizosaccharomyces pombe

INVESTIGATIONS

Three Additional Linkage Groups That Repress Transcription and Meiotic Recombination in the Mating-Type Region of Schizosaccharomyces pombe

G. Thon, A. Cohen and A. J. Klar
Present address: Department of Genetics, Institute of Molecular Biology, University of Copenhagen, {Oslash}ster Farimagsgade 2A, DK-1353 Copenhagen K, Denmark.

The mating-type genes of Schizosaccharomyces pombe are found at three locations in the same chromosomal region. These genes are in an active configuration at the mat1 locus and in an inactive configuration at the mat2 and mat3 loci. The mechanism that represses transcription of mat2 and mat3 also inactivates other promoters introduced nearby and is accompanied by a block to meiotic recombination in the mat2-mat3 interval, suggesting that this mechanism involves a particular chromatin structure. We present evidence that the transcription and recombination blocks require three newly defined trans-acting loci, clr2, clr3 and clr4, in addition to the previously identified clr1, rik1 and swi6 loci. We also investigated the role of mat2 cis-acting sequences in silencing. Four cis-acting elements that repress mat2 in a plasmid context were previously identified. Deletion of two of these elements proved to have little effect in a chromosomal context. However, when combined with mutations in trans-acting genes, deletion of the same two elements greatly enhanced mat2 expression. The observed cumulative effects suggest a redundancy in the silencing mechanism.

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				C. Blackwell, K. A. Martin, A. Greenall, A. Pidoux, R. C. Allshire, and S. K. Whitehall The Schizosaccharomyces pombe HIRA-Like Protein Hip1 Is Required for the Periodic Expression of Histone Genes and Contributes to the Function of Complex Centromeres Mol. Cell. Biol., May 15, 2004; 24(10): 4309 - 4320. [Abstract] [Full Text] [PDF]

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				A. Naresh, S. Saini, and J. Singh Identification of Uhp1, a Ubiquitinated Histone-like Protein, as a Target/Mediator of Rhp6 in Mating-type Silencing in Fission Yeast J. Biol. Chem., March 7, 2003; 278(11): 9185 - 9194. [Abstract] [Full Text] [PDF]

				E. S. Choi, H. S. Kim, Y. K. Jang, S. H. Hong, and S. D. Park Two Ubiquitin-Conjugating Enzymes, Rhp6 and UbcX, Regulate Heterochromatin Silencing in Schizosaccharomyces pombe Mol. Cell. Biol., December 1, 2002; 22(23): 8366 - 8374. [Abstract] [Full Text] [PDF]

				G. Singh and A. J. S. Klar The 2.1-kb Inverted Repeat DNA Sequences Flank the mat2,3 Silent Region in Two Species of Schizosaccharomyces and Are Involved in Epigenetic Silencing in Schizosaccharomyces pombe Genetics, October 1, 2002; 162(2): 591 - 602. [Abstract] [Full Text] [PDF]

				I. M. Hall, G. D. Shankaranarayana, K.-i. Noma, N. Ayoub, A. Cohen, and S. I. S. Grewal Establishment and Maintenance of a Heterochromatin Domain Science, September 27, 2002; 297(5590): 2232 - 2237. [Abstract] [Full Text] [PDF]

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