- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Broughton, R. E.
- Articles by Dowling, T. E.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Broughton, R. E.
- Articles by Dowling, T. E.
Genetics, Vol 138, 179-190, Copyright © 1994
INVESTIGATIONS |
Length Variation in Mitochondrial DNA of the Minnow Cyprinella spiloptera
R. E. Broughton and T. E. Dowling
Department of Zoology, Arizona State University, Tempe, Arizona 85287-1501
Length differences in animal mitochondrial DNA (mtDNA) are common, frequently due to variation in copy number of direct tandem duplications. While such duplications appear to form without great difficulty in some taxonomic groups, they appear to be relatively short-lived, as typical duplication products are geographically restricted within species and infrequently shared among species. To better understand such length variation, we have studied a tandem and direct duplication of approximately 260 bp in the control region of the cyprinid fish, Cyprinella spiloptera. Restriction site analysis of 38 individuals was used to characterize population structure and the distribution of variation in repeat copy number. This revealed two length variants, including individuals with two or three copies of the repeat, and little geographic structure among populations. No standard length (single copy) genomes were found and heteroplasmy, a common feature of length variation in other taxa, was absent. Nucleotide sequence of tandem duplications and flanking regions localized duplication junctions in the phenylalanine tRNA and near the origin of replication. The locations of these junctions and the stability of folded repeat copies support the hypothesized importance of secondary structures in models of duplication formation.
This article has been cited by other articles:
 |
|
 |
|
  S. Roques, J. A. Godoy, J. J. Negro, and F. Hiraldo Organization and Variation of the Mitochondrial Control Region in Two Vulture Species, Gypaetus barbatus and Neophron percnopterus J. Hered., July 1, 2004; 95(4): 332 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Navajas, Y. L. Conte, M. Solignac, S. Cros-Arteil, and J.-M. Cornuet The Complete Sequence of the Mitochondrial Genome of the Honeybee Ectoparasite Mite Varroa destructor (Acari: Mesostigmata) Mol. Biol. Evol., December 1, 2002; 19(12): 2313 - 2317. [Full Text] [PDF]
|
|
|
|
|
|
M. J. H. van Oppen, B. J. McDonald, B. Willis, and D. J. Miller The Evolutionary History of the Coral Genus Acropora (Scleractinia, Cnidaria) Based on a Mitochondrial and a Nuclear Marker: Reticulation, Incomplete Lineage Sorting, or Morphological Convergence? Mol. Biol. Evol., July 1, 2001; 18(7): 1315 - 1329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Nesbo, M. O. Arab, and K. S. Jakobsen Heteroplasmy, Length and Sequence Variation in the mtDNA Control Regions of Three Percid Fish Species (Perca fluviatilis, Acerina cernua, Stizostedion lucioperca) Genetics, April 1, 1998; 148(4): 1907 - 1919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Broughton, J. E. Milam, and B. A. Roe The Complete Sequence of the Zebrafish (Danio rerio) Mitochondrial Genome and Evolutionary Patterns in Vertebrate Mitochondrial DNA Genome Res., November 1, 2001; 11(11): 1958 - 1967. [Abstract] [Full Text] [PDF]
|
|