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Genetics, Vol 137, 1111-1119, Copyright © 1994
INVESTIGATIONS |
Isolation of Deficiencies in the Arabidopsis Genome by {gamma}-Irradiation of Pollen
I. Y. Vizir, M. L. Anderson, Z. A. Wilson and B. J. Mulligan
Molecular Biology Group, Department of Life Science, University of Nottingham, University Park, Nottingham NG7 2RD, England
Chromosomal deficiencies are a useful genetic tool in fine-scale genetic mapping and the integration of physical and visible marker genetic maps. Viable overlapping deficiencies may permit gene cloning by subtractive procedures and provide a means of analyzing the functional importance of different chromosomal regions. A method is described for isolation of deficiencies in the Arabidopsis genome which encompass specific loci and other extended chromosomal regions. The technique employs pollen mutagenized by {gamma}-irradiation to pollinate marker lines homozygous for recessive mutations. Deficiencies at specific loci were detected by screening for marker phenotypes in the F(1). Screening for lethal mutations in the F(1)/F(2) confirmed specific deficiencies and revealed other deficiencies that did not overlap the marker loci. Further evidence for such mutations was provided by distorted F(2) segregation of the chromosomal markers linked to putative deficiencies. Maintainable (transmissible) and non-transmissible deficiencies were demonstrated by their pattern of inheritance in subsequent generations.
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