The SUP35 Omnipotent Suppressor Gene Is Involved in the Maintenance of the Non-Mendelian Determinant [psi(+)] in the Yeast Saccharomyces cerevisiae

INVESTIGATIONS

The SUP35 Omnipotent Suppressor Gene Is Involved in the Maintenance of the Non-Mendelian Determinant [psi(+)] in the Yeast Saccharomyces cerevisiae

M. D. Ter-Avanesyan, A. R. Dagkesamanskaya, V. V. Kushnirov and V. N. Smirnov
Institute of Experimental Cardiology, Cardiology Research Center, 121552 Moscow, Russia

The SUP35 gene of yeast Saccharomyces cerevisiae encodes a 76.5-kD ribosome-associated protein (Sup35p), the C-terminal part of which exhibits a high degree of similarity to EF-1{alpha} elongation factor, while its N-terminal region is unique. Mutations in or overexpression of the SUP35 gene can generate an omnipotent suppressor effect. In the present study the SUP35 wild-type gene was replaced with deletion alleles generated in vitro that encode Sup35p lacking all or a part of the unique N-terminal region. These 5'-deletion alleles lead, in a haploid strain, simultaneously to an antisuppressor effect and to loss of the non-Mendelian determinant [psi(+)]. The antisuppressor effect is dominant while the elimination of the [psi(+)] determinant is a recessive trait. A set of the plasmid-borne deletion alleles of the SUP35 gene was tested for the ability to maintain [psi(+)]. It was shown that the first 114 amino acids of Sup35p are sufficient to maintain the [psi(+)] determinant. We propose that the Sup35p serves as a trans-acting factor required for the maintenance of [psi(+)].

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				L. J. Byrne, B. S. Cox, D. J. Cole, M. S. Ridout, B. J. T. Morgan, and M. F. Tuite Cell division is essential for elimination of the yeast [PSI+] prion by guanidine hydrochloride PNAS, July 10, 2007; 104(28): 11688 - 11693. [Abstract] [Full Text] [PDF]

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				F. Shewmaker, R. B. Wickner, and R. Tycko Amyloid of the prion domain of Sup35p has an in-register parallel beta-sheet structure PNAS, December 26, 2006; 103(52): 19754 - 19759. [Abstract] [Full Text] [PDF]

				K.-W. Park, J.-S. Hahn, Q. Fan, D. J. Thiele, and L. Li De Novo Appearance and "Strain" Formation of Yeast Prion [PSI+] Are Regulated by the Heat-Shock Transcription Factor Genetics, May 1, 2006; 173(1): 35 - 47. [Abstract] [Full Text] [PDF]

				I. S. Shkundina, V. V. Kushnirov, M. F. Tuite, and M. D. Ter-Avanesyan The Role of the N-Terminal Oligopeptide Repeats of the Yeast Sup35 Prion Protein in Propagation and Transmission of Prion Variants Genetics, February 1, 2006; 172(2): 827 - 835. [Abstract] [Full Text] [PDF]

				E. D. Ross, H. K. Edskes, M. J. Terry, and R. B. Wickner Primary sequence independence for prion formation PNAS, September 6, 2005; 102(36): 12825 - 12830. [Abstract] [Full Text] [PDF]

				N. Talarek, L. Maillet, C. Cullin, and M. Aigle The [URE3] Prion Is Not Conserved Among Saccharomyces Species Genetics, September 1, 2005; 171(1): 23 - 34. [Abstract] [Full Text] [PDF]

				M. A. Wilson, S. Meaux, R. Parker, and A. van Hoof Genetic interactions between [PSI+] and nonstop mRNA decay affect phenotypic variation PNAS, July 19, 2005; 102(29): 10244 - 10249. [Abstract] [Full Text] [PDF]

				A. B. Salnikova, D. S. Kryndushkin, V. N. Smirnov, V. V. Kushnirov, and M. D. Ter-Avanesyan Nonsense Suppression in Yeast Cells Overproducing Sup35 (eRF3) Is Caused by Its Non-heritable Amyloids J. Biol. Chem., March 11, 2005; 280(10): 8808 - 8812. [Abstract] [Full Text] [PDF]

				K. D. Allen, R. D. Wegrzyn, T. A. Chernova, S. Muller, G. P. Newnam, P. A. Winslett, K. B. Wittich, K. D. Wilkinson, and Y. O. Chernoff Hsp70 Chaperones as Modulators of Prion Life Cycle: Novel Effects of Ssa and Ssb on the Saccharomyces cerevisiae Prion [PSI+] Genetics, March 1, 2005; 169(3): 1227 - 1242. [Abstract] [Full Text] [PDF]

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				Y. Kimura, S. Koitabashi, A. Kakizuka, and T. Fujita The role of pre-existing aggregates in Hsp104-dependent polyglutamine aggregate formation and epigenetic change of yeast prions Genes Cells, August 1, 2004; 9(8): 685 - 696. [Abstract] [Full Text] [PDF]

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				M. E. Bradley and S. W. Liebman Destabilizing Interactions Among [PSI+] and [PIN+] Yeast Prion Variants Genetics, December 1, 2003; 165(4): 1675 - 1685. [Abstract] [Full Text] [PDF]

				U. Baxa, K. L. Taylor, J. S. Wall, M. N. Simon, N. Cheng, R. B. Wickner, and A. C. Steven Architecture of Ure2p Prion Filaments: THE N-TERMINAL DOMAINS FORM A CENTRAL CORE FIBER J. Biol. Chem., October 31, 2003; 278(44): 43717 - 43727. [Abstract] [Full Text] [PDF]

				A. Baudin-Baillieu, E. Fernandez-Bellot, F. Reine, E. Coissac, and C. Cullin Conservation of the Prion Properties of Ure2p through Evolution Mol. Biol. Cell, August 1, 2003; 14(8): 3449 - 3458. [Abstract] [Full Text] [PDF]

				F. Ness, P. Ferreira, B. S. Cox, and M. F. Tuite Guanidine Hydrochloride Inhibits the Generation of Prion "Seeds" but Not Prion Protein Aggregation in Yeast Mol. Cell. Biol., August 1, 2002; 22(15): 5593 - 5605. [Abstract] [Full Text] [PDF]

				S. W. Liebman Progress toward an ultimate proof of the prion hypothesis PNAS, July 9, 2002; 99(14): 9098 - 9100. [Full Text] [PDF]

				O. Namy, I. Hatin, G. Stahl, H. Liu, S. Barnay, L. Bidou, and J.-P. Rousset Gene Overexpression as a Tool for Identifying New trans-Acting Factors Involved in Translation Termination in Saccharomyces cerevisiae Genetics, June 1, 2002; 161(2): 585 - 594. [Abstract] [Full Text] [PDF]

				B. Cosson, A. Couturier, S. Chabelskaya, D. Kiktev, S. Inge-Vechtomov, M. Philippe, and G. Zhouravleva Poly(A)-Binding Protein Acts in Translation Termination via Eukaryotic Release Factor 3 Interaction and Does Not Influence [PSI+] Propagation Mol. Cell. Biol., May 15, 2002; 22(10): 3301 - 3315. [Abstract] [Full Text] [PDF]

				C. Resende, S. N. Parham, C. Tinsley, P. Ferreira, J. A. B. Duarte, and M. F. Tuite The Candida albicans Sup35p protein (CaSup35p): function, prion-like behaviour and an associated polyglutamine length polymorphism Microbiology, April 1, 2002; 148(4): 1049 - 1060. [Abstract] [Full Text] [PDF]

				K. V. Volkov, A. Yu. Aksenova, M. J. Soom, K. V. Osipov, A. V. Svitin, C. Kurischko, I. S. Shkundina, M. D. Ter-Avanesyan, S. G. Inge-Vechtomov, and L. N. Mironova Novel Non-Mendelian Determinant Involved in the Control of Translation Accuracy in Saccharomyces cerevisiae Genetics, January 1, 2002; 160(1): 25 - 36. [Abstract] [Full Text] [PDF]

				M. Schlumpberger, S. B. Prusiner, and I. Herskowitz Induction of Distinct [URE3] Yeast Prion Strains Mol. Cell. Biol., October 15, 2001; 21(20): 7035 - 7046. [Abstract] [Full Text] [PDF]

				M. A. Jensen, H. L. True, Y. O. Chernoff, and S. Lindquist Molecular Population Genetics and Evolution of a Prion-like Protein in Saccharomyces cerevisiae Genetics, October 1, 2001; 159(2): 527 - 535. [Abstract] [Full Text] [PDF]

				V. V. Speransky, K. L. Taylor, H. K. Edskes, R. B. Wickner, and A. C. Steven Prion Filament Networks in [URE3] Cells of Saccharomyces cerevisiae J. Cell Biol., June 11, 2001; 153(6): 1327 - 1336. [Abstract] [Full Text] [PDF]