- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by O'Brien, M. A.
- Articles by Taghert, P. H.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by O'Brien, M. A.
- Articles by Taghert, P. H.
Genetics, Vol 137, 121-137, Copyright © 1994
INVESTIGATIONS |
A Genetic and Molecular Analysis of the 46C Chromosomal Region Surrounding the FMRFamide Neuropeptide Gene in Drosophila melanogaster
M. A. O'Brien, M. S. Roberts and P. H. Taghert
Department of Anatomy and Neurobiology, Washington University Medical School, St. Louis, Missouri 63110
We have analyzed the FMRFamide neuropeptide gene region of Drosophila melanogaster. This gene maps to the 46C region of chromosome 2R; this interval previously was not well characterized. For this genetic and molecular analysis, we have used X-ray mutagenesis, EMS mutagenesis, and the recently reported local P element transposition method. We identified four overlapping deletions, two of which have proximal breakpoints that define a 50-60-kb region surrounding the FMRFamide gene in 46C. To this small region, we mapped three lethal complementation groups; 10 additional lethal complementation groups were mapped to more distal regions of 46CD. One of these groups corresponds to even-skipped, the other 12 are previously unidentified. Using various lines of evidence we excluded the possibility that FMRFamide corresponds to any of the three lethal complementation groups mapping to its immediate 50-60-kb vicinity. The positions of two of the three lethal complementation groups were identified with P elements using a local transposition scheme. The third lethal complementation group was excluded as being FMRFamide mutants by sequence analysis and by immunocytochemistry with proFMRFamide precursor-specific antibodies. This analysis has (1) provided a genetic map of the 46CD chromosomal region and a detailed molecular map of a portion of the 46C region and (2) provided additional evidence of the utility of local transposition for targeting nearby genes.
This article has been cited by other articles:
 |
|
 |
|
  Y Park, M Fujioka, M Kobayashi, J. Jaynes, and S Datta even skipped is required to produce a trans-acting signal for larval neuroblast proliferation that can be mimicked by ecdysone Development, January 5, 2001; 128(10): 1899 - 1909. [Abstract] [PDF]
|
|
|
|
 |
|
 R. S. Hewes, A. M. Schaefer, and P. H. Taghert The cryptocephal Gene (ATF4) Encodes Multiple Basic-Leucine Zipper Proteins Controlling Molting and Metamorphosis in Drosophila Genetics, August 1, 2000; 155(4): 1711 - 1723. [Abstract] [Full Text]
|
|
|
|
|
|
M Fujioka, Y Emi-Sarker, G. Yusibova, T Goto, and J. Jaynes Analysis of an even-skipped rescue transgene reveals both composite and discrete neuronal and early blastoderm enhancers, and multi-stripe positioning by gap gene repressor gradients Development, January 6, 1999; 126(11): 2527 - 2538. [Abstract] [PDF]
|
|
|
|
 |
|
 G. E. Carney, A. A. Wade, R. Sapra, E. S. Goldstein, and M. Bender DHR3, an ecdysone-inducible early-late gene encoding a Drosophila nuclear receptor, is required for embryogenesis PNAS, October 28, 1997; 94(22): 12024 - 12029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B A Bour, M A O'Brien, W L Lockwood, E S Goldstein, R Bodmer, P H Taghert, S M Abmayr, and H T Nguyen Drosophila MEF2, a transcription factor that is essential for myogenesis. Genes & Dev., March 15, 1995; 9(6): 730 - 741. [Abstract] [PDF]
|
|
|
|
 |
|
 B Lilly, B Zhao, G Ranganayakulu, B. Paterson, R. Schulz, and E. Olson Requirement of MADS domain transcription factor D-MEF2 for muscle formation in Drosophila Science, February 3, 1995; 267(5198): 688 - 693. [Abstract] [PDF]
|
|