Genetics, Vol 136, 1013-1023, Copyright © 1994

INVESTIGATIONS

Drosophila P Element Transposase Induces Male Recombination Additively and Without a Requirement for P Element Excision or Insertion

M. McCarron, A. Duttaroy, G. Doughty and A. Chovnick
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269-2131

P element dysgenesis-associated male recombination in Drosophila was examined with a selective system focused upon a section of the third chromosome divided into eight recombination segments. Tests compared crossing over in the presence of none, one and two doses of P({delta}2-3)(99B), a non-mobile transposase source, in the absence of a mobilizable P element target in the genome. In the presence of the P transposase source, and without a P element target, significant male recombination occurred in genomic regions physically separated from the P({delta}2-3) site. Using two doses of P({delta}2-3) without a P element target, the male recombination rate doubled, and 90% of the crossovers occurred in the pericentric region. The distribution of recombination events, in the absence of P element targets approximates that seen in studies of radiation induced mitotic crossing over and the metaphase chromosome map. Another experiment examined the effects of one dose of P({delta}2-3) on a genome with a single P element target, P(lArB)(87C9), in the third recombination segment. Crossovers increased 58-fold in the immediate region of the P element target.

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