Genetics, Vol 136, 87-91, Copyright © 1994

INVESTIGATIONS

Formamide Sensitivity: A Novel Conditional Phenotype in Yeast

A. Aguilera
Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, E-41012 Sevilla, Spain

Yeast mutants unable to grow in the presence of 3% formamide have been isolated in parallel with mutants sensitive to either 37{deg} or 6% ethanol. The number of formamide-sensitive mutations that affect different genes that can be identified from yeast cells is at least as large as the number of thermosensitive or ethanol-sensitive mutations. These mutations are of two types: those that are sensitive to formamide, temperature and/or ethanol simultaneously; and those that are specific for formamide sensitivity and show no temperature or ethanol sensitivity phenotype. Those genes susceptible to giving rise to formamide-sensitive alleles include the structural gene for DNA ligase, CDC9, and the structural gene for arginine permease, CAN1. The results indicate that formamide sensitivity can be used as a novel conditional phenotype for mutations on both essential and nonessential genes. This work also confirms that ethanol-sensitivity can be used as a conditional phenotype to identify mutations in at least as many genes as those susceptible to temperature or formamide sensitive mutations.

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