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Genetics, Vol 136, 247-254, Copyright © 1994
INVESTIGATIONS |
Insertional Mutation on Mouse Chromosome 18 With Vestibular and Craniofacial Abnormalities
C. N. Ting, D. Kohrman, D. L. Burgess, A. Boyle, R. A. Altschuler, G. Gholizadeh, L. C. Samuelson, W. Jang and M. H. Meisler
Department of Human Genetics, University of Michigan, Ann Arbor, Michigan 48109-0618 Present address: Division of Cardiology, University of Chicago, 0509 MC 5041, Chicago, Illinois 60637.
A dominant mutation was generated in transgenic mice as a consequence of insertional mutation. Heterozygous mice from transgenic line 9257 (Tg(9257)) are hyperactive with bidirectional circling behavior and have a distinctive facial appearance due to hypoplasia of the nasal bone. Morphological analysis of the inner ear revealed asymmetric abnormalities of the horizontal canal and flattening or invagination of the crista ampullaris, which can account for the circling behavior. The sensory epithelium appeared to be normal. The transgene insertion site was localized by in situ hybridization to the B1 band of mouse chromosome 18. Genetic mapping in an interspecific backcross demonstrated the gene order centromere--Tg(9257)--8.8 +/- 3.4--Grl-1, Egr-1, Fgf-1, Apc--14.7 +/- 4.3--Pdgfr. The phenotype and the mapping data suggest that the transgene may be inserted at the Twirler locus. Homozygosity for the transgene results in prenatal lethality, but compound heterozygotes carrying the Tw allele and the transgene are viable. The function of the closely linked ataxia locus is not disrupted by the transgene insertion. This insertional mutant will provide molecular access to genes located in the Twirler region of mouse chromosome 18.