- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Petersen, N. S.
- Articles by Corces, V. G.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Petersen, N. S.
- Articles by Corces, V. G.
Genetics, Vol 136, 173-182, Copyright © 1994
INVESTIGATIONS |
forked Proteins Are Components of Fiber Bundles Present in Developing Bristles of Drosophila melanogaster
N. S. Petersen, D. H. Lankenau, H. K. Mitchell, P. Young and V. G. Corces
Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071
The forked (f) gene of Drosophila melanogaster encodes six different transcripts 6.4, 5.6, 5.4, 2.5, 1.9, and 1.1 kb long. These transcripts arise by the use of alternative promoters. A polyclonal antibody raised against a domain common to all of the forked-encoded products has been used to identify forked proteins on two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and in Drosophila pupal tissues. The antibody stains fiber bundles present in bristle cells for about 15 hr during normal pupal development. Electron microscopy shows that these fibers are present from 40 to 53 hr in bristles of wild-type flies but are absent in the null f(36a) mutant. The forked protein(s) thus appear to be an essential part of the bristle fibers. The phenotype of the f(36a) mutation can be rescued by a 13-kb fragment of the forked locus containing the coding regions for the 2.5, 1.9, and 1.1-kb transcripts, suggesting that the proteins encoded by the three large forked RNAs are dispensable during bristle development. Increasing the copy number of a P[w(+),f(+)] construct containing the 13-kb fragment induces a hypermorphic bristle phenotype whose severity correlates with the number of copies of P[w(+),f(+)] present. These results indicate that alterations in the ratios among the forked proteins, or between forked products and other components of the fiber, result in abnormal assembly of the fibrillar cytoplasmic structures necessary for bristle morphogenesis.
This article has been cited by other articles:
 |
|
 |
|
  N. Ren, J. Charlton, and P. N. Adler The flare Gene, Which Encodes the AIP1 Protein of Drosophila, Functions to Regulate F-Actin Disassembly in Pupal Epidermal Cells Genetics, August 1, 2007; 176(4): 2223 - 2234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Guild, P. S. Connelly, L. Ruggiero, K. A. Vranich, and L. G. Tilney Actin Filament Bundles in Drosophila Wing Hairs: Hairs and Bristles Use Different Strategies for Assembly Mol. Biol. Cell, August 1, 2005; 16(8): 3620 - 3631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. G. Tilney, P. S. Connelly, L. Ruggiero, K. A. Vranich, G. M. Guild, and D. DeRosier The Role Actin Filaments Play in Providing the Characteristic Curved Form of Drosophila Bristles Mol. Biol. Cell, December 1, 2004; 15(12): 5481 - 5491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Naz, A J Griffith, S Riazuddin, L L Hampton, J F Battey Jr, S N Khan, S Riazuddin, E R Wilcox, and T B Friedman Mutations of ESPN cause autosomal recessive deafness and vestibular dysfunction J. Med. Genet., August 1, 2004; 41(8): 591 - 595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Bayer, S. R. Halsell, J. W. Fristrom, D. P. Kiehart, and L. von Kalm Genetic Interactions Between the RhoA and Stubble-stubbloid Loci Suggest a Role for a Type II Transmembrane Serine Protease in Intracellular Signaling During Drosophila Imaginal Disc Morphogenesis Genetics, November 1, 2003; 165(3): 1417 - 1432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. G. Tilney, P. S. Connelly, L. Ruggiero, K. A. Vranich, and G. M. Guild Actin Filament Turnover Regulated by Cross-linking Accounts for the Size, Shape, Location, and Number of Actin Bundles in Drosophila Bristles Mol. Biol. Cell, October 1, 2003; 14(10): 3953 - 3966. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Guild, P. S. Connelly, L. Ruggiero, K. A. Vranich, and L. G. Tilney Long continuous actin bundles in Drosophila bristles are constructed by overlapping short filaments J. Cell Biol., September 15, 2003; 162(6): 1069 - 1077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Fei, B. He, and P. N. Adler The growth of Drosophila bristles and laterals is not restricted to the tip or base J. Cell Sci., January 10, 2002; 115(19): 3797 - 3806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Guild, P. S. Connelly, K. A. Vranich, M. K. Shaw, and L. G. Tilney Actin filament turnover removes bundles from Drosophila bristle cells J. Cell Sci., January 2, 2002; 115(3): 641 - 653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cong, W. Geng, B. He, J. Liu, J. Charlton, and P. N. Adler The furry gene of Drosophila is important for maintaining the integrity of cellular extensions during morphogenesis Development, July 15, 2001; 128(14): 2793 - 2802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Geng, B. He, M. Wang, and P. N. Adler The tricornered Gene, Which Is Required for the Integrity of Epidermal Cell Extensions, Encodes the Drosophila Nuclear DBF2-Related Kinase Genetics, December 1, 2000; 156(4): 1817 - 1828. [Abstract] [Full Text]
|
|
|
|
|
|
K. Sawamoto, P. Winge, S. Koyama, Y. Hirota, C. Yamada, S. Miyao, S. Yoshikawa, M.-h. Jin, A. Kikuchi, and H. Okano The Drosophila Ral GTPase Regulates Developmental Cell Shape Changes through the Jun NH2-terminal Kinase Pathway J. Cell Biol., July 26, 1999; 146(2): 361 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Mathur, P Spielhofer, B Kost, and N Chua The actin cytoskeleton is required to elaborate and maintain spatial patterning during trichome cell morphogenesis in Arabidopsis thaliana Development, January 12, 1999; 126(24): 5559 - 5568. [Abstract] [PDF]
|
|
|
|
|
|
S Grieshaber and N. Petersen The Drosophila forked protein induces the formation of actin fiber bundles in vertebrate cells J. Cell Sci., January 7, 1999; 112(13): 2203 - 2211. [Abstract] [PDF]
|
|
|
|
|
|
L. G. Tilney, P. S. Connelly, K. A. Vranich, M. K. Shaw, and G. M. Guild Why Are Two Different Cross-linkers Necessary for Actin Bundle Formation In Vivo and What Does Each Cross-link Contribute? J. Cell Biol., October 5, 1998; 143(1): 121 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Bartles, L. Zheng, A. Li, A. Wierda, and B. Chen Small Espin: A Third Actin-bundling Protein and Potential Forked Protein Ortholog in Brush Border Microvilli J. Cell Biol., October 5, 1998; 143(1): 107 - 119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Cant, B. Knowles, S Mahajan-Miklos, M Heintzelman, and L Cooley Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail J. Cell Sci., January 1, 1998; 111(2): 213 - 221. [Abstract] [PDF]
|
|
|
|
|
|
J. Bartles, A Wierda, and L Zheng Identification and characterization of espin, an actin-binding protein localized to the F-actin-rich junctional plaques of Sertoli cell ectoplasmic specializations J. Cell Sci., January 6, 1996; 109(6): 1229 - 1239. [Abstract] [PDF]
|
|
|
|
|
|
E. Verheyen and L Cooley Profilin mutations disrupt multiple actin-dependent processes during Drosophila development Development, January 4, 1994; 120(4): 717 - 728. [Abstract] [PDF]
|
|
|
|
|
|
G. Wahlstrom, M. Vartiainen, L. Yamamoto, P. K. Mattila, P. Lappalainen, and T. I. Heino Twinfilin is required for actin-dependent developmental processes in Drosophila J. Cell Biol., November 26, 2001; 155(5): 787 - 796. [Abstract] [Full Text] [PDF]
|
|
|