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Genetics, Vol 135, 1023-1034, Copyright © 1993
INVESTIGATIONS |
Intragenic Dominant Suppressors of glp-1, a Gene Essential for Cell-Signaling in Caenorhabditis elegans, Support a Role for cdc10/SW16/Ankyrin Motifs in GLP-1 Function
J. L. Lissemore, P. D. Currie, C. M. Turk and E. M. Maine
Present address: Department of Biology, John Carroll University, University Heights, Ohio 44118.
The glp-1 gene product mediates cell-cell interactions required for cell fate specification during development in Caenorhabditis elegans. To identify genes that interact with glp-1, we screened for dominant suppressors of two temperature-sensitive glp-1 alleles and recovered 18 mutations that suppress both germline and embryonic glp-1 phenotypes. These dominant suppressors are tightly linked to glp-1 and do not bypass the requirement for a distal tip cell, which is thought to be the source of a signal that is received and transduced by the GLP-1 protein. Using single-strand conformation polymorphism (SSCP) analysis and DNA sequencing, we found that at least 17 suppressors are second-site intragenic revertants. The suppressors, like the original glp-1(ts) mutations, are all located in the cdc10/SWI6/ankyrin domain of GLP-1. cdc10/SWI6/ankyrin motifs have been shown to mediate specific protein-protein interactions in other polypeptides. We propose that the glp-1(ts) mutations disrupt contact between GLP-1 and an as yet unidentified target protein(s) and that the dominant suppressor mutations restore appropriate protein-protein interactions.
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