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Genetics, Vol 135, 553-564, Copyright © 1993
INVESTIGATIONS |
The Drosophila stubarista Phenotype Is Associated With a Dosage Effect of the Putative Ribosome-Associated Protein D-p40 on spineless
M. B. Melnick, E. Noll and N. Perrimon
Department of Genetics, Harvard Medical School, Boston Massachusetts 02115
We describe the molecular characterization of the Drosophila melanogaster gene stubarista (sta) that encodes the highly conserved putative ribosome-associated protein D-p40. sta maps to cytological position 2A3-B2 on the X chromosome and encodes a protein (D-p40) of 270 amino acids. D-p40 shares 63% identity with the human p40 ribosomal protein. P element-mediated transformation of a 4.4-kb genomic fragment encompassing the 1-kb transcript corresponding to D-p40 was used to rescue both a lethal (sta(2)) and a viable (sta(1)) mutation at the stubarista (sta) locus. Developmental analysis of the sta(2) mutation implicates a requirement for D-p40 during oogenesis and imaginal development, which is consistent with the expression of sta throughout development. In addition, we have analyzed the basis of the sta(1) visible phenotype which consists of shortened antennae and bristles. sta(1) is a translocation of the 1E1-2 to 2B3-4 region of the X chromosome onto the third chromosome at 89B21-C4. We provide genetic evidence that Dp(1;3)sta(1) is mutant at the spineless (ss) locus and that it is associated with partial D-p40 activity. We demonstrate that sta(1) acts as a recessive enhancer of ss; reduction in the amount of D-p40 provided by the transposed X chromosomal region of sta(1) reveals a haplo-insufficient phenotype of the otherwise recessive ss mutations. This phenomenon is reminiscent of the enhancing effect observed with Minute mutations, one of which, rp49, has previously been shown to encode a ribosomal protein.
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