Toward a Molecular Genetic Analysis of Spermatogenesis in Drosophila melanogaster: Characterization of Male-Sterile Mutants Generated by Single P Element Mutagenesis

INVESTIGATIONS

Toward a Molecular Genetic Analysis of Spermatogenesis in Drosophila melanogaster: Characterization of Male-Sterile Mutants Generated by Single P Element Mutagenesis

D. H. Castrillon, P. Gonczy, S. Alexander, R. Rawson, C. G. Eberhart, S. Viswanathan, S. DiNardo and S. A. Wasserman
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9038

We describe 83 recessive autosomal male-sterile mutations, generated by single P element mutagenesis in Drosophila melanogaster. Each mutation has been localized to a lettered subdivision of the polytene map. Reversion analyses, as well as complementation tests using available chromosomal deficiencies, indicate that the insertions are responsible for the mutant phenotypes. These mutations represent 63 complementation groups, 58 of which are required for spermatogenesis. Phenotypes of the spermatogenesis mutants were analyzed by light microscopy. Mutations in 12 loci affect germline proliferation, spermatocyte growth, or meiosis. Mutations in 46 other loci disrupt differentiation and maturation of spermatids into motile sperm. This collection of male-sterile mutants provides the basis for a molecular genetic analysis of spermatogenesis.

This article has been cited by other articles:

Z. Wu, C. Li, S. Lv, and B. Zhou
Pantothenate kinase-associated neurodegeneration: insights from a Drosophila model
Hum. Mol. Genet., October 1, 2009; 18(19): 3659 - 3672.
[Abstract] [Full Text] [PDF]

Y. M. Lin, C. L. Chung, and Y. S. Cheng
Posttranscriptional Regulation of CDC25A by BOLL Is a Conserved Fertility Mechanism Essential for Human Spermatogenesis
J. Clin. Endocrinol. Metab., July 1, 2009; 94(7): 2650 - 2657.
[Abstract] [Full Text] [PDF]

M. A. Anderson, J. N. Jodoin, E. Lee, K. G. Hales, T. S. Hays, and L. A. Lee
asunder Is a Critical Regulator of Dynein-Dynactin Localization during Drosophila Spermatogenesis
Mol. Biol. Cell, June 1, 2009; 20(11): 2709 - 2721.
[Abstract] [Full Text] [PDF]

E. Bonilla and E. Y. Xu
Identification and characterization of novel mammalian spermatogenic genes conserved from fly to human
Mol. Hum. Reprod., March 1, 2008; 14(3): 137 - 142.
[Abstract] [Full Text] [PDF]

T. M. Franklin-Dumont, C. Chatterjee, S. A. Wasserman, and S. DiNardo
A novel eIF4G homolog, Off-schedule, couples translational control to meiosis and differentiation in Drosophila spermatocytes
Development, August 1, 2007; 134(15): 2851 - 2861.
[Abstract] [Full Text] [PDF]

C. Rathke, W. M. Baarends, S. Jayaramaiah-Raja, M. Bartkuhn, R. Renkawitz, and R. Renkawitz-Pohl
Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila
J. Cell Sci., May 1, 2007; 120(9): 1689 - 1700.
[Abstract] [Full Text] [PDF]

L. M. Mikhaylova, A. M. Boutanaev, and D. I. Nurminsky
Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line
PNAS, August 8, 2006; 103(32): 11975 - 11980.
[Abstract] [Full Text] [PDF]

N. Reynolds, B. Collier, K. Maratou, V. Bingham, R. M. Speed, M. Taggart, C. A. Semple, N. K. Gray, and H. J. Cooke
Dazl binds in vivo to specific transcripts and can regulate the pre-meiotic translation of Mvh in germ cells
Hum. Mol. Genet., December 15, 2005; 14(24): 3899 - 3909.
[Abstract] [Full Text] [PDF]

A. Ghosh-Roy, B. S. Desai, and K. Ray
Dynein Light Chain 1 Regulates Dynamin-mediated F-Actin Assembly during Sperm Individualization in Drosophila
Mol. Biol. Cell, July 1, 2005; 16(7): 3107 - 3116.
[Abstract] [Full Text] [PDF]

K. D. Curtin, I. A. Meinertzhagen, and R. J. Wyman
Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture
J. Cell Sci., June 15, 2005; 118(12): 2649 - 2660.
[Abstract] [Full Text] [PDF]

Y. M. Lin, P. L. Kuo, Y. H. Lin, Y. N. Teng, and J. S. N. Lin
Messenger RNA transcripts of the meiotic regulator BOULE in the testis of azoospermic men and their application in predicting the success of sperm retrieval
Hum. Reprod., March 1, 2005; 20(3): 782 - 788.
[Abstract] [Full Text] [PDF]

K. Weber, N. Johnson, D. Champlin, and A. Patty
Many P-Element Insertions Affect Wing Shape in Drosophila melanogaster
Genetics, March 1, 2005; 169(3): 1461 - 1475.
[Abstract] [Full Text] [PDF]

Y.-M. Kuo, J. L. Duncan, S. K. Westaway, H. Yang, G. Nune, E. Y. Xu, S. J. Hayflick, and J. Gitschier
Deficiency of pantothenate kinase 2 (Pank2) in mice leads to retinal degeneration and azoospermia
Hum. Mol. Genet., January 1, 2005; 14(1): 49 - 57.
[Abstract] [Full Text] [PDF]

L. Dorstyn, K. Mills, Y. Lazebnik, and S. Kumar
The two cytochrome c species, DC3 and DC4, are not required for caspase activation and apoptosis in Drosophila cells
J. Cell Biol., November 8, 2004; 167(3): 405 - 410.
[Abstract] [Full Text] [PDF]

A. B. Milchanowski, A. L. Henkenius, M. Narayanan, V. Hartenstein, and U. Banerjee
Identification and Characterization of Genes Involved in Embryonic Crystal Cell Formation During Drosophila Hematopoiesis
Genetics, September 1, 2004; 168(1): 325 - 339.
[Abstract] [Full Text] [PDF]

B. A. Pannebakker, L. W. Beukeboom, J. J. M. van Alphen, P. M. Brakefield, and B. J. Zwaan
The Genetic Basis of Male Fertility in Relation to Haplodiploid Reproduction in Leptopilina clavipes (Hymenoptera: Figitidae)
Genetics, September 1, 2004; 168(1): 341 - 349.
[Abstract] [Full Text] [PDF]

A. Matyash, H.-R. Chung, and H. Jackle
Genome-wide Mapping of in Vivo Targets of the Drosophila Transcription Factor Kruppel
J. Biol. Chem., July 16, 2004; 279(29): 30689 - 30696.
[Abstract] [Full Text] [PDF]

S. Sun, C.-T. Ting, and C.-I Wu
The Normal Function of a Speciation Gene, Odysseus, and Its Hybrid Sterility Effect
Science, July 2, 2004; 305(5680): 81 - 83.
[Abstract] [Full Text] [PDF]

A. J. Moehring and T. F. C. Mackay
The Quantitative Genetic Basis of Male Mating Behavior in Drosophila melanogaster
Genetics, July 1, 2004; 167(3): 1249 - 1263.
[Abstract] [Full Text] [PDF]

K. Raymond, E. Bergeret, A. Avet-Rochex, R. Griffin-Shea, and M.-O. Fauvarque
A screen for modifiers of RacGAP(84C) gain-of-function in the Drosophila eye revealed the LIM kinase Cdi/TESK1 as a downstream effector of Rac1 during spermatogenesis
J. Cell Sci., June 1, 2004; 117(13): 2777 - 2789.
[Abstract] [Full Text] [PDF]

B. T. Wakimoto, D. L. Lindsley, and C. Herrera
Toward a Comprehensive Genetic Analysis of Male Fertility in Drosophila melanogaster
Genetics, May 1, 2004; 167(1): 207 - 216.
[Abstract] [Full Text] [PDF]

S. T. Harbison, A. H. Yamamoto, J. J. Fanara, K. K. Norga, and T. F. C. Mackay
Quantitative Trait Loci Affecting Starvation Resistance in Drosophila melanogaster
Genetics, April 1, 2004; 166(4): 1807 - 1823.
[Abstract] [Full Text] [PDF]

P. J. Clyne, J. S. Brotman, S. T. Sweeney, and G. Davis
Green Fluorescent Protein Tagging Drosophila Proteins at Their Native Genomic Loci With Small P Elements
Genetics, November 1, 2003; 165(3): 1433 - 1441.
[Abstract] [Full Text] [PDF]

J. Z. Morris, C. Navarro, and R. Lehmann
Identification and Analysis of Mutations in bob, Doa and Eight New Genes Required for Oocyte Specification and Development in Drosophila melanogaster
Genetics, August 1, 2003; 164(4): 1435 - 1446.
[Abstract] [Full Text] [PDF]

T. Noguchi and K. G. Miller
A role for actin dynamics in individualization during spermatogenesis in Drosophila melanogaster
Development, May 1, 2003; 130(9): 1805 - 1816.
[Abstract] [Full Text] [PDF]

A. D. Rogat and K. G. Miller
A role for myosin VI in actin dynamics at sites of membrane remodeling during Drosophila spermatogenesis
J. Cell Sci., March 14, 2003; 115(24): 4855 - 4865.
[Abstract] [Full Text] [PDF]

D. A. Dansereau, M. D. Lunke, A. Finkielsztein, M. A. Russell, and W. J. Brook
hephaestus encodes a polypyrimidine tract binding protein that regulates Notch signalling during wing development in Drosophila melanogaster
Development, March 14, 2003; 129(24): 5553 - 5566.
[Abstract] [Full Text] [PDF]

I. M. Adham, M. A. Sallam, G. Steding, M. Korabiowska, U. Brinck, S. Hoyer-Fender, C. Oh, and W. Engel
Disruption of the Pelota Gene Causes Early Embryonic Lethality and Defects in Cell Cycle Progression
Mol. Cell. Biol., February 15, 2003; 23(4): 1470 - 1476.
[Abstract] [Full Text] [PDF]

Q. Lin, D. Wu, and J. Zhou
The promoter targeting sequence facilitates and restricts a distant enhancer to a single promoter in the Drosophila embryo
Development, February 1, 2003; 130(3): 519 - 526.
[Abstract] [Full Text] [PDF]

E. Y. Xu, D. F. Lee, A. Klebes, P. J. Turek, T. B. Kornberg, and R. A. Reijo Pera
Human BOULE gene rescues meiotic defects in infertile flies
Hum. Mol. Genet., January 15, 2003; 12(2): 169 - 175.
[Abstract] [Full Text] [PDF]

H.-J. Song, J.-C. Billeter, E. Reynaud, T. Carlo, E. P. Spana, N. Perrimon, S. F. Goodwin, B. S. Baker, and B. J. Taylor
The fruitless Gene Is Required for the Proper Formation of Axonal Tracts in the Embryonic Central Nervous System of Drosophila
Genetics, December 1, 2002; 162(4): 1703 - 1724.
[Abstract] [Full Text] [PDF]

E. Betran, K. Thornton, and M. Long
Retroposed New Genes Out of the X in Drosophila
Genome Res., December 1, 2002; 12(12): 1854 - 1859.
[Abstract] [Full Text] [PDF]

B. Dauwalder, S. Tsujimoto, J. Moss, and W. Mattox
The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior
Genes & Dev., November 15, 2002; 16(22): 2879 - 2892.
[Abstract] [Full Text] [PDF]

J.-Y. Ji, M. Haghnia, C. Trusty, L. S. B. Goldstein, and G. Schubiger
A Genetic Screen for Suppressors and Enhancers of the Drosophila Cdk1-Cyclin B Identifies Maternal Factors That Regulate Microtubule and Microfilament Stability
Genetics, November 1, 2002; 162(3): 1179 - 1195.
[Abstract] [Full Text] [PDF]

S. Bulgheresi, E. Kleiner, and J. A. Knoblich
Inscuteable-dependent apical localization of the microtubule-binding protein Cornetto suggests a role in asymmetric cell division
J. Cell Sci., March 12, 2002; 114(20): 3655 - 3662.
[Abstract] [Full Text] [PDF]

J. Yager, S. Richards, D. S. Hekmat-Scafe, D. D. Hurd, V. Sundaresan, D. R. Caprette, W. M. Saxton, J. R. Carlson, and M. Stern
Control of Drosophila perineurial glial growth by interacting neurotransmitter-mediated signaling pathways
PNAS, August 17, 2001; (2001) 191107698.
[Abstract] [Full Text] [PDF]

L. A. Lee, L. K. Elfring, G. Bosco, and T. L. Orr-Weaver
A Genetic Screen for Suppressors and Enhancers of the Drosophila PAN GU Cell Cycle Kinase Identifies Cyclin B as a Target
Genetics, August 1, 2001; 158(4): 1545 - 1556.
[Abstract] [Full Text] [PDF]

A. Anand, A. Villella, L. C. Ryner, T. Carlo, S. F. Goodwin, H.-J. Song, D. A. Gailey, A. Morales, J. C. Hall, B. S. Baker, et al.
Molecular Genetic Dissection of the Sex-Specific and Vital Functions of the Drosophila melanogaster Sex Determination Gene fruitless
Genetics, August 1, 2001; 158(4): 1569 - 1595.
[Abstract] [Full Text] [PDF]

H. Butler, S. Levine, X. Wang, S. Bonyadi, G. Fu, P. Lasko, B. Suter, and R. Doerig
Map Position and Expression of the Genes in the 38 Region of Drosophila
Genetics, August 1, 2001; 158(4): 1597 - 1614.
[Abstract] [Full Text] [PDF]

E. S. Haag
Rolling back to BOULE
PNAS, June 19, 2001; 98(13): 6983 - 6985.
[Full Text] [PDF]

K. Afshar, P. Gönczy, S. DiNardo, and S. A. Wasserman
fumble Encodes a Pantothenate Kinase Homolog Required for Proper Mitosis and Meiosis in Drosophila melanogaster
Genetics, March 1, 2001; 157(3): 1267 - 1276.
[Abstract] [Full Text]

G. Lee and J. C. Hall
Abnormalities of Male-Specific FRU Protein and Serotonin Expression in the CNS of fruitless Mutants in Drosophila
J. Neurosci., January 15, 2001; 21(2): 513 - 526.
[Abstract] [Full Text] [PDF]

J. Andrews, G. G. Bouffard, C. Cheadle, J. Lü, K. G. Becker, and B. Oliver
Gene Discovery Using Computational and Microarray Analysis of Transcription in the Drosophila melanogaster Testis
Genome Res., December 1, 2000; 10(12): 2030 - 2043.
[Abstract] [Full Text]

M. V. Frolov, E. V. Benevolenskaya, and J. A. Birchler
The oxen Gene of Drosophila Encodes a Homolog of Subunit 9 of Yeast Ubiquinol-Cytochrome c Oxidoreductase Complex: Evidence for Modulation of Gene Expression in Response to Mitochondrial Activity
Genetics, December 1, 2000; 156(4): 1727 - 1736.
[Abstract] [Full Text]

A. Briscoe , Jr. and J. E. Tomkiel
Chromosomal Position Effects Reveal Different cis-Acting Requirements for rDNA Transcription and Sex Chromosome Pairing in Drosophila melanogaster
Genetics, July 1, 2000; 155(3): 1195 - 1211.
[Abstract] [Full Text]

G. Roman, J. He, and R. L. Davis
kurtz, a Novel Nonvisual Arrestin, Is an Essential Neural Gene in Drosophila
Genetics, July 1, 2000; 155(3): 1281 - 1295.
[Abstract] [Full Text]

S. F. Goodwin, B. J. Taylor, A. Villella, M. Foss, L. C. Ryner, B. S. Baker, and J. C. Hall
Aberrant Splicing and Altered Spatial Expression Patterns in fruitless Mutants of Drosophila melanogaster
Genetics, February 1, 2000; 154(2): 725 - 745.
[Abstract] [Full Text]

C. Conley, R Silburn, M. Singer, A Ralston, D Rohwer-Nutter, D. Olson, W Gelbart, and S. Blair
Crossveinless 2 contains cysteine-rich domains and is required for high levels of BMP-like activity during the formation of the cross veins in Drosophila
Development, January 9, 2000; 127(18): 3947 - 3959.
[Abstract] [PDF]

J. L. Hicks, W.-M. Deng, A. D. Rogat, K. G. Miller, and M. Bownes
Class VI Unconventional Myosin is Required for Spermatogenesis in Drosophila
Mol. Biol. Cell, December 1, 1999; 10(12): 4341 - 4353.
[Abstract] [Full Text]

D. M. Neubaum and M. F. Wolfner
Mated Drosophila melanogaster Females Require a Seminal Fluid Protein, Acp36DE, to Store Sperm Efficiently
Genetics, October 1, 1999; 153(2): 845 - 857.
[Abstract] [Full Text] [PDF]

A. C. Spradling, D. Stern, A. Beaton, E. J. Rhem, T. Laverty, N. Mozden, S. Misra, and G. M. Rubin
The Berkeley Drosophila Genome Project Gene Disruption Project: Single P-Element Insertions Mutating 25% of Vital Drosophila Genes
Genetics, September 1, 1999; 153(1): 135 - 177.
[Abstract] [Full Text]

M. Ashburner, S. Misra, J. Roote, S. E. Lewis, R. Blazej, T. Davis, C. Doyle, R. Galle, R. George, N. Harris, et al.
An Exploration of the Sequence of a 2.9-Mb Region of the Genome of Drosophila melanogaster: The Adh Region
Genetics, September 1, 1999; 153(1): 179 - 219.
[Abstract] [Full Text]

J. J. Sekelsky, K. S. McKim, L. Messina, R. L. French, W. D. Hurley, T. Arbel, G. M. Chin, B. Deneen, S. J. Force, K. L. Hari, et al.
Identification of Novel Drosophila Meiotic Genes Recovered in a P-Element Screen
Genetics, June 1, 1999; 152(2): 529 - 542.
[Abstract] [Full Text]

P. Gonczy, H. Schnabel, T. Kaletta, A. D. Amores, T. Hyman, and R. Schnabel
Dissection of Cell Division Processes in the One Cell Stage Caenorhabditis elegans Embryo by Mutational Analysis
J. Cell Biol., March 8, 1999; 144(5): 927 - 946.
[Abstract] [Full Text] [PDF]

L. Yue, T. L. Karr, D. F. Nathan, H. Swift, S. Srinivasan, and S. Lindquist
Genetic Analysis of Viable Hsp90 Alleles Reveals a Critical Role in Drosophila Spermatogenesis
Genetics, March 1, 1999; 151(3): 1065 - 1079.
[Abstract] [Full Text]

Y. Lie and P. Macdonald
Apontic binds the translational repressor Bruno and is implicated in regulation of oskar mRNA translation
Development, January 3, 1999; 126(6): 1129 - 1138.
[Abstract] [PDF]

M. L. Watson, A. R. Zinn, N. Inoue, K. D. Hess, J. Cobb, M. A. Handel, R. Halaban, C. C. Duchene, G. M. Albright, and R. W. Moreadith
Identification of morc (microrchidia), a mutation that results in arrest of spermatogenesis at an early meiotic stage in the mouse
PNAS, November 24, 1998; 95(24): 14361 - 14366.
[Abstract] [Full Text] [PDF]

P. Zhang and R. L. Stankiewicz
Y-Linked Male Sterile Mutations Induced by P Element in Drosophila melanogaster
Genetics, October 1, 1998; 150(2): 735 - 744.
[Abstract] [Full Text]

L. Davis and J. Engebrecht
Yeast dom34 Mutants Are Defective in Multiple Developmental Pathways and Exhibit Decreased Levels of Polyribosomes
Genetics, May 1, 1998; 149(1): 45 - 56.
[Abstract] [Full Text] [PDF]

J. Fabrizio, G Hime, S. Lemmon, and C Bazinet
Genetic dissection of sperm individualization in Drosophila melanogaster
Development, January 5, 1998; 125(10): 1833 - 1843.
[Abstract] [PDF]

L. Moore, H. Broihier, M Van Doren, L. Lunsford, and R Lehmann
Identification of genes controlling germ cell migration and embryonic gonad formation in Drosophila
Development, January 2, 1998; 125(4): 667 - 678.
[Abstract] [PDF]

P. J. Webster, L. Liang, C. A. Berg, P. Lasko, and P. M. Macdonald
Translational repressor bruno plays multiple roles in development and is widely conserved
Genes & Dev., October 1, 1997; 11(19): 2510 - 2521.
[Abstract] [Full Text] [PDF]

D. A. Gailey, S. Ohshima, S. J.-M. Santiago, J. M. Montez, A. R. Arellano, J. Robillo, C. A. Villarimo, L. Roberts, E. Fine, A. Villella, et al.
The muscle of Lawrence in Drosophila: A case of repeated evolutionary loss
PNAS, April 29, 1997; 94(9): 4543 - 4547.
[Abstract] [Full Text] [PDF]

G Cenci, R B Rawson, G Belloni, D H Castrillon, M Tudor, R Petrucci, M L Goldberg, S A Wasserman, and M Gatti
UbcD1, a Drosophila ubiquitin-conjugating enzyme required for proper telomere behavior.
Genes & Dev., April 1, 1997; 11(7): 863 - 875.
[Abstract] [PDF]

P Gonczy, E Matunis, and S DiNardo
bag-of-marbles and benign gonial cell neoplasm act in the germline to restrict proliferation during Drosophila spermatogenesis
Development, January 11, 1997; 124(21): 4361 - 4371.
[Abstract] [PDF]

M Boyle, N Bonini, and S DiNardo
Expression and function of clift in the development of somatic gonadal precursors within the Drosophila mesoderm
Development, January 3, 1997; 124(5): 971 - 982.
[Abstract] [PDF]

M A Lilly and A C Spradling
The Drosophila endocycle is controlled by Cyclin E and lacks a checkpoint ensuring S-phase completion.
Genes & Dev., October 1, 1996; 10(19): 2514 - 2526.
[Abstract] [PDF]

P Gonczy and S DiNardo
The germ line regulates somatic cyst cell proliferation and fate during Drosophila spermatogenesis
Development, January 8, 1996; 122(8): 2437 - 2447.
[Abstract] [PDF]

T. Lin, S Viswanathan, C Wood, P. Wilson, N Wolf, and M. Fuller
Coordinate developmental control of the meiotic cell cycle and spermatid differentiation in Drosophila males
Development, January 4, 1996; 122(4): 1331 - 1341.
[Abstract] [PDF]

C. Armstrong, D. Mann, N Berndt, and P. Cohen
Drosophila PPY, a novel male specific protein serine/threonine phosphatase localised in somatic cells of the testis
J. Cell Sci., January 11, 1995; 108(11): 3367 - 3375.
[Abstract] [PDF]

C. Eberhart and S. Wasserman
The pelota locus encodes a protein required for meiotic cell division: an analysis of G2/M arrest in Drosophila spermatogenesis
Development, January 10, 1995; 121(10): 3477 - 3486.
[Abstract] [PDF]

B. Taylor and L. Knittel
Sex-specific differentiation of a male-specific abdominal muscle, the Muscle of Lawrence, is abnormal in hydroxyurea-treated and in fruitless male flies
Development, January 9, 1995; 121(9): 3079 - 3088.
[Abstract] [PDF]

J. Hall
The mating of a fly
Science, June 17, 1994; 264(5166): 1702 - 1714.
[Abstract] [PDF]

G Cenci, S Bonaccorsi, C Pisano, F Verni, and M Gatti
Chromatin and microtubule organization during premeiotic, meiotic and early postmeiotic stages of Drosophila melanogaster spermatogenesis
J. Cell Sci., January 12, 1994; 107(12): 3521 - 3534.
[Abstract] [PDF]

D. Castrillon and S. Wasserman
Diaphanous is required for cytokinesis in Drosophila and shares domains of similarity with the products of the limb deformity gene
Development, January 12, 1994; 120(12): 3367 - 3377.
[Abstract] [PDF]

E. Verheyen and L Cooley
Profilin mutations disrupt multiple actin-dependent processes during Drosophila development
Development, January 4, 1994; 120(4): 717 - 728.
[Abstract] [PDF]

J. Yager, S. Richards, D. S. Hekmat-Scafe, D. D. Hurd, V. Sundaresan, D. R. Caprette, W. M. Saxton, J. R. Carlson, and M. Stern
Control of Drosophila perineurial glial growth by interacting neurotransmitter-mediated signaling pathways
PNAS, August 28, 2001; 98(18): 10445 - 10450.
[Abstract] [Full Text] [PDF]

				Y. M. Lin, C. L. Chung, and Y. S. Cheng Posttranscriptional Regulation of CDC25A by BOLL Is a Conserved Fertility Mechanism Essential for Human Spermatogenesis J. Clin. Endocrinol. Metab., July 1, 2009; 94(7): 2650 - 2657. [Abstract] [Full Text] [PDF]

				M. A. Anderson, J. N. Jodoin, E. Lee, K. G. Hales, T. S. Hays, and L. A. Lee asunder Is a Critical Regulator of Dynein-Dynactin Localization during Drosophila Spermatogenesis Mol. Biol. Cell, June 1, 2009; 20(11): 2709 - 2721. [Abstract] [Full Text] [PDF]

				E. Bonilla and E. Y. Xu Identification and characterization of novel mammalian spermatogenic genes conserved from fly to human Mol. Hum. Reprod., March 1, 2008; 14(3): 137 - 142. [Abstract] [Full Text] [PDF]

				T. M. Franklin-Dumont, C. Chatterjee, S. A. Wasserman, and S. DiNardo A novel eIF4G homolog, Off-schedule, couples translational control to meiosis and differentiation in Drosophila spermatocytes Development, August 1, 2007; 134(15): 2851 - 2861. [Abstract] [Full Text] [PDF]

				C. Rathke, W. M. Baarends, S. Jayaramaiah-Raja, M. Bartkuhn, R. Renkawitz, and R. Renkawitz-Pohl Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila J. Cell Sci., May 1, 2007; 120(9): 1689 - 1700. [Abstract] [Full Text] [PDF]

				L. M. Mikhaylova, A. M. Boutanaev, and D. I. Nurminsky Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line PNAS, August 8, 2006; 103(32): 11975 - 11980. [Abstract] [Full Text] [PDF]

				N. Reynolds, B. Collier, K. Maratou, V. Bingham, R. M. Speed, M. Taggart, C. A. Semple, N. K. Gray, and H. J. Cooke Dazl binds in vivo to specific transcripts and can regulate the pre-meiotic translation of Mvh in germ cells Hum. Mol. Genet., December 15, 2005; 14(24): 3899 - 3909. [Abstract] [Full Text] [PDF]

				A. Ghosh-Roy, B. S. Desai, and K. Ray Dynein Light Chain 1 Regulates Dynamin-mediated F-Actin Assembly during Sperm Individualization in Drosophila Mol. Biol. Cell, July 1, 2005; 16(7): 3107 - 3116. [Abstract] [Full Text] [PDF]

				K. D. Curtin, I. A. Meinertzhagen, and R. J. Wyman Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture J. Cell Sci., June 15, 2005; 118(12): 2649 - 2660. [Abstract] [Full Text] [PDF]

				Y. M. Lin, P. L. Kuo, Y. H. Lin, Y. N. Teng, and J. S. N. Lin Messenger RNA transcripts of the meiotic regulator BOULE in the testis of azoospermic men and their application in predicting the success of sperm retrieval Hum. Reprod., March 1, 2005; 20(3): 782 - 788. [Abstract] [Full Text] [PDF]

				K. Weber, N. Johnson, D. Champlin, and A. Patty Many P-Element Insertions Affect Wing Shape in Drosophila melanogaster Genetics, March 1, 2005; 169(3): 1461 - 1475. [Abstract] [Full Text] [PDF]

				Y.-M. Kuo, J. L. Duncan, S. K. Westaway, H. Yang, G. Nune, E. Y. Xu, S. J. Hayflick, and J. Gitschier Deficiency of pantothenate kinase 2 (Pank2) in mice leads to retinal degeneration and azoospermia Hum. Mol. Genet., January 1, 2005; 14(1): 49 - 57. [Abstract] [Full Text] [PDF]

				L. Dorstyn, K. Mills, Y. Lazebnik, and S. Kumar The two cytochrome c species, DC3 and DC4, are not required for caspase activation and apoptosis in Drosophila cells J. Cell Biol., November 8, 2004; 167(3): 405 - 410. [Abstract] [Full Text] [PDF]

				A. B. Milchanowski, A. L. Henkenius, M. Narayanan, V. Hartenstein, and U. Banerjee Identification and Characterization of Genes Involved in Embryonic Crystal Cell Formation During Drosophila Hematopoiesis Genetics, September 1, 2004; 168(1): 325 - 339. [Abstract] [Full Text] [PDF]

				B. A. Pannebakker, L. W. Beukeboom, J. J. M. van Alphen, P. M. Brakefield, and B. J. Zwaan The Genetic Basis of Male Fertility in Relation to Haplodiploid Reproduction in Leptopilina clavipes (Hymenoptera: Figitidae) Genetics, September 1, 2004; 168(1): 341 - 349. [Abstract] [Full Text] [PDF]

				A. Matyash, H.-R. Chung, and H. Jackle Genome-wide Mapping of in Vivo Targets of the Drosophila Transcription Factor Kruppel J. Biol. Chem., July 16, 2004; 279(29): 30689 - 30696. [Abstract] [Full Text] [PDF]

				S. Sun, C.-T. Ting, and C.-I Wu The Normal Function of a Speciation Gene, Odysseus, and Its Hybrid Sterility Effect Science, July 2, 2004; 305(5680): 81 - 83. [Abstract] [Full Text] [PDF]

				A. J. Moehring and T. F. C. Mackay The Quantitative Genetic Basis of Male Mating Behavior in Drosophila melanogaster Genetics, July 1, 2004; 167(3): 1249 - 1263. [Abstract] [Full Text] [PDF]

				K. Raymond, E. Bergeret, A. Avet-Rochex, R. Griffin-Shea, and M.-O. Fauvarque A screen for modifiers of RacGAP(84C) gain-of-function in the Drosophila eye revealed the LIM kinase Cdi/TESK1 as a downstream effector of Rac1 during spermatogenesis J. Cell Sci., June 1, 2004; 117(13): 2777 - 2789. [Abstract] [Full Text] [PDF]

				B. T. Wakimoto, D. L. Lindsley, and C. Herrera Toward a Comprehensive Genetic Analysis of Male Fertility in Drosophila melanogaster Genetics, May 1, 2004; 167(1): 207 - 216. [Abstract] [Full Text] [PDF]

				S. T. Harbison, A. H. Yamamoto, J. J. Fanara, K. K. Norga, and T. F. C. Mackay Quantitative Trait Loci Affecting Starvation Resistance in Drosophila melanogaster Genetics, April 1, 2004; 166(4): 1807 - 1823. [Abstract] [Full Text] [PDF]

				P. J. Clyne, J. S. Brotman, S. T. Sweeney, and G. Davis Green Fluorescent Protein Tagging Drosophila Proteins at Their Native Genomic Loci With Small P Elements Genetics, November 1, 2003; 165(3): 1433 - 1441. [Abstract] [Full Text] [PDF]

				J. Z. Morris, C. Navarro, and R. Lehmann Identification and Analysis of Mutations in bob, Doa and Eight New Genes Required for Oocyte Specification and Development in Drosophila melanogaster Genetics, August 1, 2003; 164(4): 1435 - 1446. [Abstract] [Full Text] [PDF]

				T. Noguchi and K. G. Miller A role for actin dynamics in individualization during spermatogenesis in Drosophila melanogaster Development, May 1, 2003; 130(9): 1805 - 1816. [Abstract] [Full Text] [PDF]

				A. D. Rogat and K. G. Miller A role for myosin VI in actin dynamics at sites of membrane remodeling during Drosophila spermatogenesis J. Cell Sci., March 14, 2003; 115(24): 4855 - 4865. [Abstract] [Full Text] [PDF]

				D. A. Dansereau, M. D. Lunke, A. Finkielsztein, M. A. Russell, and W. J. Brook hephaestus encodes a polypyrimidine tract binding protein that regulates Notch signalling during wing development in Drosophila melanogaster Development, March 14, 2003; 129(24): 5553 - 5566. [Abstract] [Full Text] [PDF]

				I. M. Adham, M. A. Sallam, G. Steding, M. Korabiowska, U. Brinck, S. Hoyer-Fender, C. Oh, and W. Engel Disruption of the Pelota Gene Causes Early Embryonic Lethality and Defects in Cell Cycle Progression Mol. Cell. Biol., February 15, 2003; 23(4): 1470 - 1476. [Abstract] [Full Text] [PDF]

				Q. Lin, D. Wu, and J. Zhou The promoter targeting sequence facilitates and restricts a distant enhancer to a single promoter in the Drosophila embryo Development, February 1, 2003; 130(3): 519 - 526. [Abstract] [Full Text] [PDF]

				E. Y. Xu, D. F. Lee, A. Klebes, P. J. Turek, T. B. Kornberg, and R. A. Reijo Pera Human BOULE gene rescues meiotic defects in infertile flies Hum. Mol. Genet., January 15, 2003; 12(2): 169 - 175. [Abstract] [Full Text] [PDF]

				H.-J. Song, J.-C. Billeter, E. Reynaud, T. Carlo, E. P. Spana, N. Perrimon, S. F. Goodwin, B. S. Baker, and B. J. Taylor The fruitless Gene Is Required for the Proper Formation of Axonal Tracts in the Embryonic Central Nervous System of Drosophila Genetics, December 1, 2002; 162(4): 1703 - 1724. [Abstract] [Full Text] [PDF]