Genetics, Vol 135, 117-125, Copyright © 1993

INVESTIGATIONS

Mutations in the Protein Phosphatase 1 Gene at 87B Can Differentially Affect Suppression of Position-Effect Variegation and Mitosis in Drosophila melanogaster

K. Baksa, H. Morawietz, V. Dombradi, M. Axton, H. Taubert, G. Szabo, I. Torok, A. Udvardy, H. Gyurkovics, B. Szoor, D. Glover, G. Reuter and J. Gausz
Institute of Biochemistry, Biological Research Centre, H-6701 Szeged, Hungary

The suppressor of position effect variegation (PEV) locus Su-var(3)6 maps to 87B5-10. The breakpoints of deficiencies that define this interval have been placed on a 250-kb molecular map of the region. The locus is allelic to the ck19 complementation group previously shown to encode a type 1 serine-threonine protein phosphatase (PP1) catalytic subunit. When introduced into flies by P element-mediated transformation, a 5.8-kb genomic fragment carrying this gene overcomes the suppressor phenotype of Su-var(3)6(01) and recessive lethality of all mutations of the locus. Four of the mutant alleles at the locus show a broad correlation between high levels of suppression of PEV, a high frequency of aberrant mitosis and low PP1 activity in larval extracts. However, some alleles with low PP1 activity show weak suppression of PEV with a high frequency of abnormal mitosis, whereas others show strong suppression of PEV with normal mitosis. The basis for these disparate phenotypes is discussed.

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