Transposition Pattern of the Maize Element Ds in Arabidopsis thaliana

INVESTIGATIONS

Transposition Pattern of the Maize Element Ds in Arabidopsis thaliana

I. Bancroft and C. Dean
AFRC Institute of Plant Science Research, Cambridge Laboratory, John Innes Centre, Colney, Norwich NR4 7UJ, England

As part of establishing an efficient transposon tagging system in Arabidopsis using the maize elements Ac and Ds, we have analyzed the inheritance and pattern of Ds transposition in four independent Arabidopsis transformants. A low proportion (33%) of plants inheriting the marker used to monitor excision contained a transposed Ds. Selection for the transposed Ds increased this to at least 49%. Overall, 68% of Ds transpositions inherited with the excision marker were to genetically linked sites; however, the distribution of transposed elements varied around the different donor sites. Mapping of transposed Ds elements that were genetically unlinked to the donor site showed that a proportion (3 of 11 tested) integrated into sites which were still physically linked.

This article has been cited by other articles:

G. Yang, C. F. Weil, and S. R. Wessler
A Rice Tc1/Mariner-Like Element Transposes in Yeast
PLANT CELL, October 1, 2006; 18(10): 2469 - 2478.
[Abstract] [Full Text] [PDF]

K.-S. Kim, S. Kustu, and W. Inwood
Natural History of Transposition in the Green Alga Chlamydomonas reinhardtii: Use of the AMT4 Locus as an Experimental System
Genetics, August 1, 2006; 173(4): 2005 - 2019.
[Abstract] [Full Text] [PDF]

M. Nozawa, M. Kumagai, T. Aotsuka, and K. Tamura
Unusual Evolution of Interspersed Repeat Sequences in the Drosophila ananassae Subgroup
Mol. Biol. Evol., May 1, 2006; 23(5): 981 - 987.
[Abstract] [Full Text] [PDF]

I. Szeverenyi, R. Ramamoorthy, Z. W. Teo, H. F. Luan, Z. G. Ma, and S. Ramachandran
Large-scale Systematic Study on Stability of the Ds Element and Timing of Transposition in Rice
Plant Cell Physiol., January 1, 2006; 47(1): 84 - 95.
[Abstract] [Full Text] [PDF]

B. Nishal, T. Tantikanjana, and V. Sundaresan
An Inducible Targeted Tagging System for Localized Saturation Mutagenesis in Arabidopsis
Plant Physiology, January 1, 2005; 137(1): 3 - 12.
[Abstract] [Full Text] [PDF]

X. Pan, Y. Li, and L. Stein
Site Preferences of Insertional Mutagenesis Agents in Arabidopsis
Plant Physiology, January 1, 2005; 137(1): 168 - 175.
[Abstract] [Full Text] [PDF]

K. Lipkow, N. Buisine, and R. Chalmers
Promiscuous Target Interactions in the mariner Transposon Himar1
J. Biol. Chem., November 19, 2004; 279(47): 48569 - 48575.
[Abstract] [Full Text] [PDF]

D. R. Page, C. Kohler, J. A. da Costa-Nunes, C. Baroux, J. M. Moore, and U. Grossniklaus
Intrachromosomal excision of a hybrid Ds element induces large genomic deletions in Arabidopsis
PNAS, March 2, 2004; 101(9): 2969 - 2974.
[Abstract] [Full Text] [PDF]

P. R. Muskett, L. Clissold, A. Marocco, P. S. Springer, R. Martienssen, and C. Dean
A Resource of Mapped Dissociation Launch Pads for Targeted Insertional Mutagenesis in the Arabidopsis Genome
Plant Physiology, June 1, 2003; 132(2): 506 - 516.
[Abstract] [Full Text] [PDF]

K. M. Bollman, M. J. Aukerman, M.-Y. Park, C. Hunter, T. Z. Berardini, and R. S. Poethig
HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis
Development, April 15, 2003; 130(8): 1493 - 1504.
[Abstract] [Full Text] [PDF]

S. I. Wright, Q. H. Le, D. J. Schoen, and T. E. Bureau
Population Dynamics of an Ac-like Transposable Element in Self- and Cross-Pollinating Arabidopsis
Genetics, July 1, 2001; 158(3): 1279 - 1288.
[Abstract] [Full Text] [PDF]

G. K. Agrawal, M. Yamazaki, M. Kobayashi, R. Hirochika, A. Miyao, and H. Hirochika
Screening of the Rice Viviparous Mutants Generated by Endogenous Retrotransposon Tos17 Insertion. Tagging of a Zeaxanthin Epoxidase Gene and a Novel OsTATC Gene
Plant Physiology, March 1, 2001; 125(3): 1248 - 1257.
[Abstract] [Full Text]

P. S. Springer
Gene Traps: Tools for Plant Development and Genomics
PLANT CELL, July 1, 2000; 12(7): 1007 - 1020.
[Abstract] [Full Text]

D. Weigel, J. H. Ahn, M. A. Blázquez, J. O. Borevitz, S. K. Christensen, C. Fankhauser, C. Ferrándiz, I. Kardailsky, E. J. Malancharuvil, M. M. Neff, et al.
Activation Tagging in Arabidopsis
Plant Physiology, April 1, 2000; 122(4): 1003 - 1014.
[Abstract] [Full Text]

W. P. Fitzmaurice, L. V. Nguyen, E. A. Wernsman, W. F. Thompson, and M. A. Conkling
Transposon Tagging of the Sulfur Gene of Tobacco Using Engineered Maize Ac/Ds Elements
Genetics, December 1, 1999; 153(4): 1919 - 1928.
[Abstract] [Full Text]

A. F. Tissier, S. Marillonnet, V. Klimyuk, K. Patel, M. A. Torres, G. Murphy, and J. D. G. Jones
Multiple Independent Defective Suppressor-mutator Transposon Insertions in Arabidopsis: A Tool for Functional Genomics
PLANT CELL, October 1, 1999; 11(10): 1841 - 1852.
[Abstract] [Full Text]

E. Speulman, P. L. J. Metz, G. van Arkel, B. te Lintel Hekkert, W. J. Stiekema, and A. Pereira
A Two-Component Enhancer-Inhibitor Transposon Mutagenesis System for Functional Analysis of the Arabidopsis Genome
PLANT CELL, October 1, 1999; 11(10): 1853 - 1866.
[Abstract] [Full Text]

I. Fridborg, S. Kuusk, T. Moritz, and E. Sundberg
The Arabidopsis Dwarf Mutant shi Exhibits Reduced Gibberellin Responses Conferred by Overexpression of a New Putative Zinc Finger Protein
PLANT CELL, June 1, 1999; 11(6): 1019 - 1032.
[Abstract] [Full Text]

Q. Migheli, R. Laugé, J.-M. Davière, C. Gerlinger, F. Kaper, T. Langin, and M.-J. Daboussi
Transposition of the Autonomous Fot1 Element in the Filamentous Fungus Fusarium oxysporum
Genetics, March 1, 1999; 151(3): 1005 - 1013.
[Abstract] [Full Text]

P. Carol, D. Stevenson, C. Bisanz, J. Breitenbach, G. Sandmann, R. Mache, G. Coupland, and M. Kuntz
Mutations in the Arabidopsis Gene IMMUTANS Cause a Variegated Phenotype by Inactivating a Chloroplast Terminal Oxidase Associated with Phytoene Desaturation
PLANT CELL, January 1, 1999; 11(1): 57 - 68.
[Abstract] [Full Text]

R. A. Martienssen
Functional genomics: Probing plant gene function and expression with transposons
PNAS, March 3, 1998; 95(5): 2021 - 2026.
[Abstract] [Full Text] [PDF]

J. Peng, P. Carol, D. E. Richards, K. E. King, R. J. Cowling, G. P. Murphy, and N. P. Harberd
The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses
Genes & Dev., December 1, 1997; 11(23): 3194 - 3205.
[Abstract] [Full Text] [PDF]

C. Machida, H. Onouchi, J. Koizumi, S. Hamada, E. Semiarti, S. Torikai, and Y. Machida
Characterization of the transposition pattern of the Ac element in Arabidopsis thaliana using endonuclease I-SceI
PNAS, August 5, 1997; 94(16): 8675 - 8680.
[Abstract] [Full Text] [PDF]

V Sundaresan, P Springer, T Volpe, S Haward, J D Jones, C Dean, H Ma, and R Martienssen
Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements.
Genes & Dev., July 15, 1995; 9(14): 1797 - 1810.
[Abstract] [PDF]

R Noll, M. Sturtevant, R. Gollapudi, and E Bier
New functions of the Drosophila rhomboid gene during embryonic and adult development are revealed by a novel genetic method, enhancer piracy
Development, January 8, 1994; 120(8): 2329 - 2338.
[Abstract] [PDF]

				K.-S. Kim, S. Kustu, and W. Inwood Natural History of Transposition in the Green Alga Chlamydomonas reinhardtii: Use of the AMT4 Locus as an Experimental System Genetics, August 1, 2006; 173(4): 2005 - 2019. [Abstract] [Full Text] [PDF]

				M. Nozawa, M. Kumagai, T. Aotsuka, and K. Tamura Unusual Evolution of Interspersed Repeat Sequences in the Drosophila ananassae Subgroup Mol. Biol. Evol., May 1, 2006; 23(5): 981 - 987. [Abstract] [Full Text] [PDF]

				I. Szeverenyi, R. Ramamoorthy, Z. W. Teo, H. F. Luan, Z. G. Ma, and S. Ramachandran Large-scale Systematic Study on Stability of the Ds Element and Timing of Transposition in Rice Plant Cell Physiol., January 1, 2006; 47(1): 84 - 95. [Abstract] [Full Text] [PDF]

				B. Nishal, T. Tantikanjana, and V. Sundaresan An Inducible Targeted Tagging System for Localized Saturation Mutagenesis in Arabidopsis Plant Physiology, January 1, 2005; 137(1): 3 - 12. [Abstract] [Full Text] [PDF]

				X. Pan, Y. Li, and L. Stein Site Preferences of Insertional Mutagenesis Agents in Arabidopsis Plant Physiology, January 1, 2005; 137(1): 168 - 175. [Abstract] [Full Text] [PDF]

				K. Lipkow, N. Buisine, and R. Chalmers Promiscuous Target Interactions in the mariner Transposon Himar1 J. Biol. Chem., November 19, 2004; 279(47): 48569 - 48575. [Abstract] [Full Text] [PDF]

				D. R. Page, C. Kohler, J. A. da Costa-Nunes, C. Baroux, J. M. Moore, and U. Grossniklaus Intrachromosomal excision of a hybrid Ds element induces large genomic deletions in Arabidopsis PNAS, March 2, 2004; 101(9): 2969 - 2974. [Abstract] [Full Text] [PDF]

				P. R. Muskett, L. Clissold, A. Marocco, P. S. Springer, R. Martienssen, and C. Dean A Resource of Mapped Dissociation Launch Pads for Targeted Insertional Mutagenesis in the Arabidopsis Genome Plant Physiology, June 1, 2003; 132(2): 506 - 516. [Abstract] [Full Text] [PDF]

				K. M. Bollman, M. J. Aukerman, M.-Y. Park, C. Hunter, T. Z. Berardini, and R. S. Poethig HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis Development, April 15, 2003; 130(8): 1493 - 1504. [Abstract] [Full Text] [PDF]

				S. I. Wright, Q. H. Le, D. J. Schoen, and T. E. Bureau Population Dynamics of an Ac-like Transposable Element in Self- and Cross-Pollinating Arabidopsis Genetics, July 1, 2001; 158(3): 1279 - 1288. [Abstract] [Full Text] [PDF]

				G. K. Agrawal, M. Yamazaki, M. Kobayashi, R. Hirochika, A. Miyao, and H. Hirochika Screening of the Rice Viviparous Mutants Generated by Endogenous Retrotransposon Tos17 Insertion. Tagging of a Zeaxanthin Epoxidase Gene and a Novel OsTATC Gene Plant Physiology, March 1, 2001; 125(3): 1248 - 1257. [Abstract] [Full Text]

				P. S. Springer Gene Traps: Tools for Plant Development and Genomics PLANT CELL, July 1, 2000; 12(7): 1007 - 1020. [Abstract] [Full Text]

				D. Weigel, J. H. Ahn, M. A. Blázquez, J. O. Borevitz, S. K. Christensen, C. Fankhauser, C. Ferrándiz, I. Kardailsky, E. J. Malancharuvil, M. M. Neff, et al. Activation Tagging in Arabidopsis Plant Physiology, April 1, 2000; 122(4): 1003 - 1014. [Abstract] [Full Text]

				W. P. Fitzmaurice, L. V. Nguyen, E. A. Wernsman, W. F. Thompson, and M. A. Conkling Transposon Tagging of the Sulfur Gene of Tobacco Using Engineered Maize Ac/Ds Elements Genetics, December 1, 1999; 153(4): 1919 - 1928. [Abstract] [Full Text]

				A. F. Tissier, S. Marillonnet, V. Klimyuk, K. Patel, M. A. Torres, G. Murphy, and J. D. G. Jones Multiple Independent Defective Suppressor-mutator Transposon Insertions in Arabidopsis: A Tool for Functional Genomics PLANT CELL, October 1, 1999; 11(10): 1841 - 1852. [Abstract] [Full Text]

				E. Speulman, P. L. J. Metz, G. van Arkel, B. te Lintel Hekkert, W. J. Stiekema, and A. Pereira A Two-Component Enhancer-Inhibitor Transposon Mutagenesis System for Functional Analysis of the Arabidopsis Genome PLANT CELL, October 1, 1999; 11(10): 1853 - 1866. [Abstract] [Full Text]

				I. Fridborg, S. Kuusk, T. Moritz, and E. Sundberg The Arabidopsis Dwarf Mutant shi Exhibits Reduced Gibberellin Responses Conferred by Overexpression of a New Putative Zinc Finger Protein PLANT CELL, June 1, 1999; 11(6): 1019 - 1032. [Abstract] [Full Text]

				Q. Migheli, R. Laugé, J.-M. Davière, C. Gerlinger, F. Kaper, T. Langin, and M.-J. Daboussi Transposition of the Autonomous Fot1 Element in the Filamentous Fungus Fusarium oxysporum Genetics, March 1, 1999; 151(3): 1005 - 1013. [Abstract] [Full Text]

				P. Carol, D. Stevenson, C. Bisanz, J. Breitenbach, G. Sandmann, R. Mache, G. Coupland, and M. Kuntz Mutations in the Arabidopsis Gene IMMUTANS Cause a Variegated Phenotype by Inactivating a Chloroplast Terminal Oxidase Associated with Phytoene Desaturation PLANT CELL, January 1, 1999; 11(1): 57 - 68. [Abstract] [Full Text]

				R. A. Martienssen Functional genomics: Probing plant gene function and expression with transposons PNAS, March 3, 1998; 95(5): 2021 - 2026. [Abstract] [Full Text] [PDF]

				J. Peng, P. Carol, D. E. Richards, K. E. King, R. J. Cowling, G. P. Murphy, and N. P. Harberd The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses Genes & Dev., December 1, 1997; 11(23): 3194 - 3205. [Abstract] [Full Text] [PDF]

				C. Machida, H. Onouchi, J. Koizumi, S. Hamada, E. Semiarti, S. Torikai, and Y. Machida Characterization of the transposition pattern of the Ac element in Arabidopsis thaliana using endonuclease I-SceI PNAS, August 5, 1997; 94(16): 8675 - 8680. [Abstract] [Full Text] [PDF]

				V Sundaresan, P Springer, T Volpe, S Haward, J D Jones, C Dean, H Ma, and R Martienssen Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements. Genes & Dev., July 15, 1995; 9(14): 1797 - 1810. [Abstract] [PDF]

				R Noll, M. Sturtevant, R. Gollapudi, and E Bier New functions of the Drosophila rhomboid gene during embryonic and adult development are revealed by a novel genetic method, enhancer piracy Development, January 8, 1994; 120(8): 2329 - 2338. [Abstract] [PDF]