Genetics, Vol 134, 717-728, Copyright © 1993

INVESTIGATIONS

Isogenic Strain Construction and Gene Mapping in Candida albicans

W. A. Fonzi and M. Y. Irwin
Department of Microbiology and Molecular Genetics, California College of Medicine, University of California, Irvine, Irvine, California 92717

Genetic manipulation of Candida albicans is constrained by its diploid genome and asexual life cycle. Recessive mutations are not expressed when heterozygous and undesired mutations introduced in the course of random mutagenesis cannot be removed by genetic back-crossing. To circumvent these problems, we developed a genotypic screen that permitted identification of a heterozygous recessive mutation at the URA3 locus. The mutation was introduced by targeted mutagenesis, homologous integration of transforming DNA, to avoid introduction of extraneous mutations. The ura3 mutation was rendered homozygous by a second round of transformation resulting in a Ura(-) strain otherwise isogenic with the parental clinical isolate. Subsequent mutation of the Ura(-) strain was achieved by targeted mutagenesis using the URA3 gene as a selectable marker. URA3 selection was used repeatedly for the sequential introduction of mutations by flanking the URA3 gene with direct repeats of the Salmonella typhimurium hisG gene. Spontaneous intrachromosomal recombination between the flanking repeats excised the URA3 gene restoring a Ura(-) phenotype. These Ura(-) segregants were selected on 5-fluoroorotic acid-containing medium and used in the next round of mutagenesis. To permit the physical mapping of disrupted genes, the 18-bp recognition sequence of the endonuclease I-SceI was incorporated into the hisG repeats. Site-specific cleavage of the chromosome with I-SceI revealed the position of the integrated sequences.


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
A. Miyazato, K. Nakamura, N. Yamamoto, H. M. Mora-Montes, M. Tanaka, Y. Abe, D. Tanno, K. Inden, X. Gang, K. Ishii, et al.
Toll-Like Receptor 9-Dependent Activation of Myeloid Dendritic Cells by Deoxynucleic Acids from Candida albicans
Infect. Immun., July 1, 2009; 77(7): 3056 - 3064.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. A. Johnston, K. E. Eberle, J. E. Sturtevant, and G. E. Palmer
Role for Endosomal and Vacuolar GTPases in Candida albicans Pathogenesis
Infect. Immun., June 1, 2009; 77(6): 2343 - 2355.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
C. Stichternoth and J. F. Ernst
Hypoxic Adaptation by Efg1 Regulates Biofilm Formation by Candida albicans
Appl. Envir. Microbiol., June 1, 2009; 75(11): 3663 - 3672.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
E. Moreno-Ruiz, G. Ortu, P. W. J. de Groot, F. Cottier, C. Loussert, M.-C. Prevost, C. de Koster, F. M. Klis, S. Goyard, and C. d'Enfert
The GPI-modified proteins Pga59 and Pga62 of Candida albicans are required for cell wall integrity
Microbiology, June 1, 2009; 155(6): 2004 - 2020.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. Znaidi, K. S. Barker, S. Weber, A.-M. Alarco, T. T. Liu, G. Boucher, P. D. Rogers, and M. Raymond
Identification of the Candida albicans Cap1p Regulon
Eukaryot. Cell, June 1, 2009; 8(6): 806 - 820.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
G. Kayingo, A. Martins, R. Andrie, L. Neves, C. Lucas, and B. Wong
A permease encoded by STL1 is required for active glycerol uptake by Candida albicans
Microbiology, May 1, 2009; 155(5): 1547 - 1557.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Ghosh, D. H. M. L. P. Navarathna, D. D. Roberts, J. T. Cooper, A. L. Atkin, T. M. Petro, and K. W. Nickerson
Arginine-Induced Germ Tube Formation in Candida albicans Is Essential for Escape from Murine Macrophage Line RAW 264.7
Infect. Immun., April 1, 2009; 77(4): 1596 - 1605.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
S. P. Saville, A. L. Lazzell, A. K. Chaturvedi, C. Monteagudo, and J. L. Lopez-Ribot
Efficacy of a Genetically Engineered Candida albicans tet-NRG1 Strain as an Experimental Live Attenuated Vaccine against Hematogenously Disseminated Candidiasis
Clin. Vaccine Immunol., March 1, 2009; 16(3): 430 - 432.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
E. Balish
A URA3 null mutant of Candida albicans (CAI-4) causes oro-oesophageal and gastric candidiasis and is lethal for gnotobiotic, transgenic mice (Tg{epsilon}26) that are deficient in both natural killer and T cells
J. Med. Microbiol., March 1, 2009; 58(3): 290 - 295.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
D. M. MacCallum, L. Castillo, K. Nather, C. A. Munro, A. J. P. Brown, N. A. R. Gow, and F. C. Odds
Property Differences among the Four Major Candida albicans Strain Clades
Eukaryot. Cell, March 1, 2009; 8(3): 373 - 387.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
R. Alonso-Monge, S. Carvaihlo, C. Nombela, E. Rial, and J. Pla
The Hog1 MAP kinase controls respiratory metabolism in the fungal pathogen Candida albicans
Microbiology, February 1, 2009; 155(2): 413 - 423.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
N. Dabas, S. Schneider, and J. Morschhauser
Mutational Analysis of the Candida albicans Ammonium Permease Mep2p Reveals Residues Required for Ammonium Transport and Signaling
Eukaryot. Cell, February 1, 2009; 8(2): 147 - 160.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
J. Gibson, A. Sood, and D. A. Hogan
Pseudomonas aeruginosa-Candida albicans Interactions: Localization and Fungal Toxicity of a Phenazine Derivative
Appl. Envir. Microbiol., January 15, 2009; 75(2): 504 - 513.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Shen, L. E. Cowen, A. M. Griffin, L. Chan, and J. R. Kohler
The Candida albicans pescadillo homolog is required for normal hypha-to-yeast morphogenesis and yeast proliferation
PNAS, December 30, 2008; 105(52): 20918 - 20923.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
H. M. Mora-Montes, O. Bader, E. Lopez-Romero, S. Zinker, P. Ponce-Noyola, B. Hube, N. A. R. Gow, and A. Flores-Carreon
Kex2 protease converts the endoplasmic reticulum {alpha}1,2-mannosidase of Candida albicans into a soluble cytosolic form
Microbiology, December 1, 2008; 154(12): 3782 - 3794.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
J. R. Naglik, D. Moyes, J. Makwana, P. Kanzaria, E. Tsichlaki, G. Weindl, A. R. Tappuni, C. A. Rodgers, A. J. Woodman, S. J. Challacombe, et al.
Quantitative expression of the Candida albicans secreted aspartyl proteinase gene family in human oral and vaginal candidiasis
Microbiology, November 1, 2008; 154(11): 3266 - 3280.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
U. Lermann and J. Morschhauser
Secreted aspartic proteases are not required for invasion of reconstituted human epithelia by Candida albicans
Microbiology, November 1, 2008; 154(11): 3281 - 3295.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
H. Badrane, M. H. Nguyen, S. Cheng, V. Kumar, H. Derendorf, K. A. Iczkowski, and C. J. Clancy
The Candida albicans phosphatase Inp51p interacts with the EH domain protein Irs4p, regulates phosphatidylinositol-4,5-bisphosphate levels and influences hyphal formation, the cell integrity pathway and virulence
Microbiology, November 1, 2008; 154(11): 3296 - 3308.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. J. Carman, S. Vylkova, and M. C. Lorenz
Role of Acetyl Coenzyme A Synthesis and Breakdown in Alternative Carbon Source Utilization in Candida albicans
Eukaryot. Cell, October 1, 2008; 7(10): 1733 - 1741.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
N. Hanaoka, Y. Takano, K. Shibuya, H. Fugo, Y. Uehara, and M. Niimi
Identification of the Putative Protein Phosphatase Gene PTC1 as a Virulence-Related Gene Using a Silkworm Model of Candida albicans Infection
Eukaryot. Cell, October 1, 2008; 7(10): 1640 - 1648.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Ramsdale, L. Selway, D. Stead, J. Walker, Z. Yin, S. M. Nicholls, J. Crowe, E. M. Sheils, and A. J.P. Brown
MNL1 Regulates Weak Acid-induced Stress Responses of the Fungal Pathogen Candida albicans
Mol. Biol. Cell, October 1, 2008; 19(10): 4393 - 4403.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Lu, C. Su, X. Mao, P. P. Raniga, H. Liu, and J. Chen
Efg1-mediated Recruitment of NuA4 to Promoters Is Required for Hypha-specific Swi/Snf Binding and Activation in Candida albicans
Mol. Biol. Cell, October 1, 2008; 19(10): 4260 - 4272.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Marcil, C. Gadoury, J. Ash, J. Zhang, A. Nantel, and M. Whiteway
Analysis of PRA1 and Its Relationship to Candida albicans- Macrophage Interactions
Infect. Immun., September 1, 2008; 76(9): 4345 - 4358.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Cambi, M. G. Netea, H. M. Mora-Montes, N. A. R. Gow, S. V. Hato, D. W. Lowman, B.-J. Kullberg, R. Torensma, D. L. Williams, and C. G. Figdor
Dendritic Cell Interaction with Candida albicans Critically Depends on N-Linked Mannan
J. Biol. Chem., July 18, 2008; 283(29): 20590 - 20599.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
M. Clement, J. Tremblay, M. Lange, J. Thibodeau, and P. Belhumeur
Purification and Identification of Bovine Cheese Whey Fatty Acids Exhibiting In Vitro Antifungal Activity
J Dairy Sci, July 1, 2008; 91(7): 2535 - 2544.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
B. G. Oliver, P. M. Silver, and T. C. White
Polyene susceptibility is dependent on nitrogen source in the opportunistic pathogen Candida albicans
J. Antimicrob. Chemother., June 1, 2008; 61(6): 1302 - 1308.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
B. W. Kebaara, M. L. Langford, D. H. M. L. P. Navarathna, R. Dumitru, K. W. Nickerson, and A. L. Atkin
Candida albicans Tup1 Is Involved in Farnesol-Mediated Inhibition of Filamentous-Growth Induction
Eukaryot. Cell, June 1, 2008; 7(6): 980 - 987.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
A. T. Coste, M. Ramsdale, F. Ischer, and D. Sanglard
Divergent functions of three Candida albicans zinc-cluster transcription factors (CTA4, ASG1 and CTF1) complementing pleiotropic drug resistance in Saccharomyces cerevisiae
Microbiology, May 1, 2008; 154(5): 1491 - 1501.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. Cuellar-Cruz, M. Briones-Martin-del-Campo, I. Canas-Villamar, J. Montalvo-Arredondo, L. Riego-Ruiz, I. Castano, and A. De Las Penas
High Resistance to Oxidative Stress in the Fungal Pathogen Candida glabrata Is Mediated by a Single Catalase, Cta1p, and Is Controlled by the Transcription Factors Yap1p, Skn7p, Msn2p, and Msn4p
Eukaryot. Cell, May 1, 2008; 7(5): 814 - 825.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
C. S. Noffz, V. Liedschulte, K. Lengeler, and J. F. Ernst
Functional Mapping of the Candida albicans Efg1 Regulator
Eukaryot. Cell, May 1, 2008; 7(5): 881 - 893.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. M. Maidan, L. De Rop, M. Relloso, R. Diez-Orejas, J. M. Thevelein, and P. Van Dijck
Combined Inactivation of the Candida albicans GPR1 and TPS2 Genes Results in Avirulence in a Mouse Model for Systemic Infection
Infect. Immun., April 1, 2008; 76(4): 1686 - 1694.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Banerjee, D. S. Thompson, A. Lazzell, P. L. Carlisle, C. Pierce, C. Monteagudo, J. L. Lopez-Ribot, and D. Kadosh
UME6, a Novel Filament-specific Regulator of Candida albicans Hyphal Extension and Virulence
Mol. Biol. Cell, April 1, 2008; 19(4): 1354 - 1365.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Gonzalez-Novo, J. Correa-Bordes, L. Labrador, M. Sanchez, C. R. Vazquez de Aldana, and J. Jimenez
Sep7 Is Essential to Modify Septin Ring Dynamics and Inhibit Cell Separation during Candida albicans Hyphal Growth
Mol. Biol. Cell, April 1, 2008; 19(4): 1509 - 1518.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
B. G. Oliver, P. M. Silver, C. Marie, S. J. Hoot, S. E. Leyde, and T. C. White
Tetracycline alters drug susceptibility in Candida albicans and other pathogenic fungi
Microbiology, March 1, 2008; 154(3): 960 - 970.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
H. Zhou and M. C. Lorenz
Carnitine acetyltransferases are required for growth on non-fermentable carbon sources but not for pathogenesis in Candida albicans
Microbiology, February 1, 2008; 154(2): 500 - 509.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
G. J. Sosinska, P. W. J. de Groot, M. J. Teixeira de Mattos, H. L. Dekker, C. G. de Koster, K. J. Hellingwerf, and F. M. Klis
Hypoxic conditions and iron restriction affect the cell-wall proteome of Candida albicans grown under vagina-simulative conditions
Microbiology, February 1, 2008; 154(2): 510 - 520.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Poltermann, A. Kunert, M. von der Heide, R. Eck, A. Hartmann, and P. F. Zipfel
Gpm1p Is a Factor H-, FHL-1-, and Plasminogen-binding Surface Protein of Candida albicans
J. Biol. Chem., December 28, 2007; 282(52): 37537 - 37544.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
H. M. Mora-Montes, S. Bates, M. G. Netea, D. F. Diaz-Jimenez, E. Lopez-Romero, S. Zinker, P. Ponce-Noyola, B. J. Kullberg, A. J. P. Brown, F. C. Odds, et al.
Endoplasmic Reticulum {alpha}-Glycosidases of Candida albicans Are Required for N Glycosylation, Cell Wall Integrity, and Normal Host-Fungus Interaction
Eukaryot. Cell, December 1, 2007; 6(12): 2184 - 2193.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
B. G. Oliver, J. L. Song, J. H. Choiniere, and T. C. White
cis-Acting Elements within the Candida albicans ERG11 Promoter Mediate the Azole Response through Transcription Factor Upc2p
Eukaryot. Cell, December 1, 2007; 6(12): 2231 - 2239.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
Y. Li, C. Su, X. Mao, F. Cao, and J. Chen
Roles of Candida albicans Sfl1 in Hyphal Development
Eukaryot. Cell, November 1, 2007; 6(11): 2112 - 2121.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
T. T. Liu, S. Znaidi, K. S. Barker, L. Xu, R. Homayouni, S. Saidane, J. Morschhauser, A. Nantel, M. Raymond, and P. D. Rogers
Genome-Wide Expression and Location Analyses of the Candida albicans Tac1p Regulon
Eukaryot. Cell, November 1, 2007; 6(11): 2122 - 2138.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Gacser, F. Stehr, C. Kroger, L. Kredics, W. Schafer, and J. D. Nosanchuk
Lipase 8 Affects the Pathogenesis of Candida albicans
Infect. Immun., October 1, 2007; 75(10): 4710 - 4718.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
N. Chauhan, M. Kruppa, and R. Calderone
The Ssk1p Response Regulator and Chk1p Histidine Kinase Mutants of Candida albicans Are Hypersensitive to Fluconazole and Voriconazole
Antimicrob. Agents Chemother., October 1, 2007; 51(10): 3747 - 3751.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Coste, A. Selmecki, A. Forche, D. Diogo, M.-E. Bougnoux, C. d'Enfert, J. Berman, and D. Sanglard
Genotypic Evolution of Azole Resistance Mechanisms in Sequential Candida albicans Isolates
Eukaryot. Cell, October 1, 2007; 6(10): 1889 - 1904.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. Vylkova, W. S. Jang, W. Li, N. Nayyar, and M. Edgerton
Histatin 5 Initiates Osmotic Stress Response in Candida albicans via Activation of the Hog1 Mitogen-Activated Protein Kinase Pathway
Eukaryot. Cell, October 1, 2007; 6(10): 1876 - 1888.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. J. Wolyniak and P. Sundstrom
Role of Actin Cytoskeletal Dynamics in Activation of the Cyclic AMP Pathway and HWP1 Gene Expression in Candida albicans
Eukaryot. Cell, October 1, 2007; 6(10): 1824 - 1840.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. D. Vinces and C. A. Kumamoto
The morphogenetic regulator Czf1p is a DNA-binding protein that regulates white opaque switching in Candida albicans
Microbiology, September 1, 2007; 153(9): 2877 - 2884.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
B. E. Jackson, K. R. Wilhelmus, and B. Hube
The Role of Secreted Aspartyl Proteinases in Candida albicans Keratitis
Invest. Ophthalmol. Vis. Sci., August 1, 2007; 48(8): 3559 - 3565.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
O. Kinclova-Zimmermannova and H. Sychrova
Plasma-membrane Cnh1 Na+/H+ antiporter regulates potassium homeostasis in Candida albicans
Microbiology, August 1, 2007; 153(8): 2603 - 2612.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
C. Ding and G. Butler
Development of a Gene Knockout System in Candida parapsilosis Reveals a Conserved Role for BCR1 in Biofilm Formation
Eukaryot. Cell, August 1, 2007; 6(8): 1310 - 1319.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Thevissen, K. R. Ayscough, A. M. Aerts, W. Du, K. De Brucker, E. M. K. Meert, J. Ausma, M. Borgers, B. P. A. Cammue, and I. E. J. A. Francois
Miconazole Induces Changes in Actin Cytoskeleton prior to Reactive Oxygen Species Induction in Yeast
J. Biol. Chem., July 27, 2007; 282(30): 21592 - 21597.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Hsu, M. J. McEachern, A. T. Dandjinou, Y. Tzfati, E. Orr, E. H. Blackburn, and N. F. Lue
Telomerase core components protect Candida telomeres from aberrant overhang accumulation
PNAS, July 10, 2007; 104(28): 11682 - 11687.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
X. Zhao, S.-H. Oh, and L. L. Hoyer
Unequal contribution of ALS9 alleles to adhesion between Candida albicans and human vascular endothelial cells
Microbiology, July 1, 2007; 153(7): 2342 - 2350.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
S. Jezewski, M. von der Heide, S. Poltermann, A. Hartl, W. Kunkel, P. F. Zipfel, and R. Eck
Role of the Vps34p-interacting protein Ade5,7p in hyphal growth and virulence of Candida albicans
Microbiology, July 1, 2007; 153(7): 2351 - 2362.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. R. Erb-Downward and M. C. Noverr
Characterization of Prostaglandin E2 Production by Candida albicans
Infect. Immun., July 1, 2007; 75(7): 3498 - 3505.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
B. E. Jackson, B. M. Mitchell, and K. R. Wilhelmus
Corneal Virulence of Candida albicans Strains Deficient in Tup1-Regulated Genes
Invest. Ophthalmol. Vis. Sci., June 1, 2007; 48(6): 2535 - 2539.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Bates, J. M. de la Rosa, D. M. MacCallum, A. J. P. Brown, N. A. R. Gow, and F. C. Odds
Candida albicans Iff11, a Secreted Protein Required for Cell Wall Structure and Virulence
Infect. Immun., June 1, 2007; 75(6): 2922 - 2928.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
K. Nielsen, A. L. De Obaldia, and J. Heitman
Cryptococcus neoformans Mates on Pigeon Guano: Implications for the Realized Ecological Niche and Globalization
Eukaryot. Cell, June 1, 2007; 6(6): 949 - 959.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
Y. Pedreno, P. Gonzalez-Parraga, M. Martinez-Esparza, R. Sentandreu, E. Valentin, and J.-C. Arguelles
Disruption of the Candida albicans ATC1 gene encoding a cell-linked acid trehalase decreases hypha formation and infectivity without affecting resistance to oxidative stress
Microbiology, May 1, 2007; 153(5): 1372 - 1381.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. Enjalbert, D. M. MacCallum, F. C. Odds, and A. J. P. Brown
Niche-Specific Activation of the Oxidative Stress Response by the Pathogenic Fungus Candida albicans
Infect. Immun., May 1, 2007; 75(5): 2143 - 2151.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
N. Dabas and J. Morschhauser
Control of Ammonium Permease Expression and Filamentous Growth by the GATA Transcription Factors GLN3 and GAT1 in Candida albicans
Eukaryot. Cell, May 1, 2007; 6(5): 875 - 888.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
L. Edwards-Ingram, P. Gitsham, N. Burton, G. Warhurst, I. Clarke, D. Hoyle, S. G. Oliver, and L. Stateva
Genotypic and Physiological Characterization of Saccharomyces boulardii, the Probiotic Strain of Saccharomyces cerevisiae
Appl. Envir. Microbiol., April 15, 2007; 73(8): 2458 - 2467.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
D. Kunze, D. MacCallum, F. C. Odds, and B. Hube
Multiple functions of DOA1 in Candida albicans
Microbiology, April 1, 2007; 153(4): 1026 - 1041.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. Argimon, J. A. Wishart, R. Leng, S. Macaskill, A. Mavor, T. Alexandris, S. Nicholls, A. W. Knight, B. Enjalbert, R. Walmsley, et al.
Developmental Regulation of an Adhesin Gene during Cellular Morphogenesis in the Fungal Pathogen Candida albicans
Eukaryot. Cell, April 1, 2007; 6(4): 682 - 692.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. Kim, M. J. Wolyniak, J. F. Staab, and P. Sundstrom
A 368-Base-Pair cis-Acting HWP1 Promoter Region, HCR, of Candida albicans Confers Hypha-Specific Gene Regulation and Binds Architectural Transcription Factors Nhp6 and Gcf1p
Eukaryot. Cell, April 1, 2007; 6(4): 693 - 709.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Levitin, A. Marcil, G. Tettweiler, M. J. Laforest, U. Oberholzer, A. M. Alarco, D. Y. Thomas, P. Lasko, and M. Whiteway
Drosophila melanogaster Thor and Response to Candida albicans Infection
Eukaryot. Cell, April 1, 2007; 6(4): 658 - 663.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
I. Bruzual, P. Riggle, S. Hadley, and C. A. Kumamoto
Biofilm formation by fluconazole-resistant Candida albicans strains is inhibited by fluconazole
J. Antimicrob. Chemother., March 1, 2007; 59(3): 441 - 450.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. A. Ramirez and M. C. Lorenz
Mutations in Alternative Carbon Utilization Pathways in Candida albicans Attenuate Virulence and Confer Pleiotropic Phenotypes
Eukaryot. Cell, February 1, 2007; 6(2): 280 - 290.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. Martchenko, A. Levitin, and M. Whiteway
Transcriptional Activation Domains of the Candida albicans Gcn4p and Gal4p Homologs
Eukaryot. Cell, February 1, 2007; 6(2): 291 - 301.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Wang, S. Lane, Z. Tian, A. Sharon, I. Hazan, and H. Liu
Temporal and Spatial Control of HGC1 Expression Results in Hgc1 Localization to the Apical Cells of Hyphae in Candida albicans
Eukaryot. Cell, February 1, 2007; 6(2): 253 - 261.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Pardini, P. W. J. De Groot, A. T. Coste, M. Karababa, F. M. Klis, C. G. de Koster, and D. Sanglard
The CRH Family Coding for Cell Wall Glycosylphosphatidylinositol Proteins with a Predicted Transglycosidase Domain Affects Cell Wall Organization and Virulence of Candida albicans
J. Biol. Chem., December 29, 2006; 281(52): 40399 - 40411.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
X. Chen and C. A. Kumamoto
A conserved G protein (Drg1p) plays a role in regulation of invasive filamentation in Candida albicans
Microbiology, December 1, 2006; 152(12): 3691 - 3700.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
B. Rognon, Z. Kozovska, A. T. Coste, G. Pardini, and D. Sanglard
Identification of promoter elements responsible for the regulation of MDR1 from Candida albicans, a major facilitator transporter involved in azole resistance
Microbiology, December 1, 2006; 152(12): 3701 - 3722.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
Y. Porat, K. Marynka, A. Tam, D. Steinberg, and A. Mor
Acyl-Substituted Dermaseptin S4 Derivatives with Improved Bactericidal Properties, Including on Oral Microflora
Antimicrob. Agents Chemother., December 1, 2006; 50(12): 4153 - 4160.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. M. Mulhern, M. E. Logue, and G. Butler
Candida albicans Transcription Factor Ace2 Regulates Metabolism and Is Required for Filamentation in Hypoxic Conditions
Eukaryot. Cell, December 1, 2006; 5(12): 2001 - 2013.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
P. J. Riggle and C. A. Kumamoto
Transcriptional Regulation of MDR1, Encoding a Drug Efflux Determinant, in Fluconazole-Resistant Candida albicans Strains through an Mcm1p Binding Site
Eukaryot. Cell, December 1, 2006; 5(12): 1957 - 1968.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
T. Prasad, A. Chandra, C. K. Mukhopadhyay, and R. Prasad
Unexpected Link between Iron and Drug Resistance of Candida spp.: Iron Depletion Enhances Membrane Fluidity and Drug Diffusion, Leading to Drug-Susceptible Cells
Antimicrob. Agents Chemother., November 1, 2006; 50(11): 3597 - 3606.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Kaneko, T. Umeyama, Y. Utena-Abe, S. Yamagoe, M. Niimi, and Y. Uehara
Tcc1p, a Novel Protein Containing the Tetratricopeptide Repeat Motif, Interacts with Tup1p To Regulate Morphological Transition and Virulence in Candida albicans
Eukaryot. Cell, November 1, 2006; 5(11): 1894 - 1905.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
K. Franke, M. Nguyen, A. Hartl, H.-M. Dahse, G. Vogl, R. Wurzner, P. F. Zipfel, W. Kunkel, and R. Eck
The vesicle transport protein Vac1p is required for virulence of Candida albicans.
Microbiology, October 1, 2006; 152(Pt 10): 3111 - 3121.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
V. Brown, J. A. Sexton, and M. Johnston
A Glucose Sensor in Candida albicans.
Eukaryot. Cell, October 1, 2006; 5(10): 1726 - 1737.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
T. Srikantha, A. R. Borneman, K. J. Daniels, C. Pujol, W. Wu, M. R. Seringhaus, M. Gerstein, S. Yi, M. Snyder, and D. R. Soll
TOS9 Regulates White-Opaque Switching in Candida albicans
Eukaryot. Cell, October 1, 2006; 5(10): 1674 - 1687.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
P. C.G. Rida, A. Nishikawa, G. Y. Won, and N. Dean
Yeast-to-Hyphal Transition Triggers Formin-dependent Golgi Localization to the Growing Tip in Candida albicans
Mol. Biol. Cell, October 1, 2006; 17(10): 4364 - 4378.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. E. Zordan, D. J. Galgoczy, and A. D. Johnson
From the Cover: Epigenetic properties of white-opaque switching in Candida albicans are based on a self-sustaining transcriptional feedback loop
PNAS, August 22, 2006; 103(34): 12807 - 12812.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. S. Li, J. N. Sun, K. Okamoto-Shibayama, and M. Edgerton
Candida albicans Cell Wall Ssa Proteins Bind and Facilitate Import of Salivary Histatin 5 Required for Toxicity
J. Biol. Chem., August 11, 2006; 281(32): 22453 - 22463.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
X. Zhao, K. J. Daniels, S.-H. Oh, C. B. Green, K. M. Yeater, D. R. Soll, and L. L. Hoyer
Candida albicans Als3p is required for wild-type biofilm formation on silicone elastomer surfaces.
Microbiology, August 1, 2006; 152(Pt 8): 2287 - 2299.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
U. Oberholzer, A. Nantel, J. Berman, and M. Whiteway
Transcript Profiles of Candida albicans Cortical Actin Patch Mutants Reflect Their Cellular Defects: Contribution of the Hog1p and Mkc1p Signaling Pathways.
Eukaryot. Cell, August 1, 2006; 5(8): 1252 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Rodaki, T. Young, and A. J. P. Brown
Effects of Depleting the Essential Central Metabolic Enzyme Fructose-1,6-Bisphosphate Aldolase on the Growth and Viability of Candida albicans: Implications for Antifungal Drug Target Discovery.
Eukaryot. Cell, August 1, 2006; 5(8): 1371 - 1377.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
D. Hiller, S. Stahl, and J. Morschhauser
Multiple cis-Acting Sequences Mediate Upregulation of the MDR1 Efflux Pump in a Fluconazole-Resistant Clinical Candida albicans Isolate.
Antimicrob. Agents Chemother., July 1, 2006; 50(7): 2300 - 2308.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. D. Vinces, C. Haas, and C. A. Kumamoto
Expression of the Candida albicans Morphogenesis Regulator Gene CZF1 and Its Regulation by Efg1p and Czf1p
Eukaryot. Cell, May 1, 2006; 5(5): 825 - 835.
[Abstract] [Full Text] [PDF]

Home page
 MycologiaHome page
M. Wellington, M. Anaul, and K. E. Rustchenko
5-fluoro-orotic acid induces chromosome alterations in genetically manipulated strains of Candida albicans.
Mycologia, May 1, 2006; 98(3): 393 - 398.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
D. Hiller, D. Sanglard, and J. Morschhauser
Overexpression of the MDR1 Gene Is Sufficient To Confer Increased Resistance to Toxic Compounds in Candida albicans.
Antimicrob. Agents Chemother., April 1, 2006; 50(4): 1365 - 1371.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. Umeyama, A. Kaneko, H. Watanabe, A. Hirai, Y. Uehara, M. Niimi, and M. Azuma
Deletion of the CaBIG1 Gene Reduces {beta}-1,6-Glucan Synthesis, Filamentation, Adhesion, and Virulence in Candida albicans
Infect. Immun., April 1, 2006; 74(4): 2373 - 2381.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. Liu, M. D. Healy, B. A. Dougherty, K. M. Esposito, T. C. Maurice, C. E. Mazzucco, R. E. Bruccoleri, D. B. Davison, M. Frosco, J. F. Barrett, et al.
Conserved fungal genes as potential targets for broad-spectrum antifungal drug discovery.
Eukaryot. Cell, April 1, 2006; 5(4): 638 - 649.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Coste, V. Turner, F. Ischer, J. Morschhauser, A. Forche, A. Selmecki, J. Berman, J. Bille, and D. Sanglard
A Mutation in Tac1p, a Transcription Factor Regulating CDR1 and CDR2, Is Coupled With Loss of Heterozygosity at Chromosome 5 to Mediate Antifungal Resistance in Candida albicans
Genetics, April 1, 2006; 172(4): 2139 - 2156.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Clemente-Blanco, A. Gonzalez-Novo, F. Machin, D. Caballero-Lima, L. Aragon, M. Sanchez, C. R. V. de Aldana, J. Jimenez, and J. Correa-Bordes
The Cdc14p phosphatase affects late cell-cycle events and morphogenesis in Candida albicans
J. Cell Sci., March 15, 2006; 119(6): 1130 - 1143.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
T. Miyazaki, Y. Miyazaki, K. Izumikawa, H. Kakeya, S. Miyakoshi, J. E. Bennett, and S. Kohno
Fluconazole Treatment Is Effective against a Candida albicans erg3/erg3 Mutant In Vivo Despite In Vitro Resistance
Antimicrob. Agents Chemother., February 1, 2006; 50(2): 580 - 586.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
C. Bai, X.-L. Xu, F.-Y. Chan, R. T. H. Lee, and Y. Wang
MNN5 Encodes an Iron-Regulated {alpha}-1,2-Mannosyltransferase Important for Protein Glycosylation, Cell Wall Integrity, Morphogenesis, and Virulence in Candida albicans
Eukaryot. Cell, February 1, 2006; 5(2): 238 - 247.
[Abstract] [Full Text] [PDF]